New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 867463	37 (1.010)	4 (0.110)	4/268	4.6	10.3%	coding (896/966 nt)	iaaA	Isoaspartyl peptidase
	?	NC_000913_3_pae_tpiA	= 867471	34 (0.970)					coding (904/966 nt)	iaaA	Isoaspartyl peptidase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGACCAGCAGAAAA  >  NC_000913_3_pae_tpiA/867318‑867604                                                                                                                                                  |                                                                                                                                              tACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTgggg                                                                                                                                            <  1:421608/149‑1 (MQ=255)      cggATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACg                                                                                                                                       >  1:426558/1‑149 (MQ=255)       ggATTAAGGCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCTCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGc                                                                                                                                      <  2:623283/149‑1 (MQ=255)          ttAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATTGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAgg                                                                                                                                   <  1:555426/149‑1 (MQ=255)                ctcGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATAc                                                                                                                             <  1:60956/149‑1 (MQ=255)                 tcGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAAACGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCTTGGGGCTACCCAGGCGATAcc                                                                                                                            >  2:157796/1‑148 (MQ=255)                         gCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAaccac                                                                                                                    >  1:68906/1‑149 (MQ=255)                           cTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCg                                                                                                                  >  1:230064/1‑149 (MQ=255)                                   gggTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTAc                                                                                                          <  1:157779/149‑1 (MQ=255)                                           aTGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGaaaa                                                                                                  <  2:403598/149‑1 (MQ=255)                                              gAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAgg                                                                                               >  2:260006/1‑149 (MQ=255)                                                         tGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCC‑ACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGc                                                                                   >  2:579042/1‑149 (MQ=255)                                                           cgcTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCCCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGcc                                                                                  >  2:228512/1‑149 (MQ=255)                                                              ttGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCcaca                                                                               <  2:225452/149‑1 (MQ=255)                                                                       gCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGtgatga                                                                      >  2:333743/1‑149 (MQ=255)                                                                             gCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGa                                                                <  1:333743/149‑1 (MQ=255)                                                                              cTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACAGCGAAGGAATGTATCGCGCCGGGGGCGACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGAt                                                               >  2:381952/1‑149 (MQ=255)                                                                                  aTCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCg                                                           >  2:240899/1‑149 (MQ=255)                                                                                      cTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGGGCCTTGGGCTGCGCAGGCGATCCGCCAACCACCGGTATCTACCGTGAAAAATGGGACACCGTTTCCACACAGTGCTTAACTTGCTGCCGCTaa                                                       >  2:2164/1‑149 (MQ=255)                                                                                       tATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACCCCGTTGGCACACCGTGATGAACTTGCTGCCGCTAAt                                                      >  1:35825/1‑149 (MQ=255)                                                                                         tCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATg                                                              <  1:600994/139‑1 (MQ=255)                                                                                         tCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATg                                                              >  2:600994/1‑139 (MQ=255)                                                                                                           tCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCt                                  <  2:341021/149‑1 (MQ=255)                                                                                                           tCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCAACGGTATGTCCGGTGAAGAAGGGGACACCGTTGCCACACAGAGATGAACTGAACGCCGGTAATGTGCTGGCGGTTgcgaacag                                  >  2:384608/1‑142 (MQ=255)                                                                                                           gtgcgcTACCGTTTAACACCGAAGGAATGTACCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCt                                  <  2:206691/147‑1 (MQ=255)                                                                                                                     gTTTAACCCCGAAGGAATGAATCGCGCCGGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGcc                        <  2:483410/149‑1 (MQ=255)                                                                                                                      tttAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACGGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATCCCGGTAATGTGCTGGCGGTTGAAAATCGGAATATTGCCt                       >  2:313954/1‑149 (MQ=255)                                                                                                                      tttAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATGTGAATATTGCCt                       >  2:493597/1‑149 (MQ=255)                                                                                                                         aaCACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATAc                                                                                                                             <  1:62257/44‑1 (MQ=255)                                                                                                                         aaCACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATAc                                                                                                                             >  2:62257/1‑44 (MQ=255)                                                                                                                            acCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAGATCTGAATATTGCCTTTATGc                 >  1:235382/1‑149 (MQ=255)                                                                                                                              cGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAGAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAg               <  1:197228/149‑1 (MQ=255)                                                                                                                                 aGGAATGTACCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGAc            <  2:68906/149‑1 (MQ=255)                                                                                                                                  tgAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGAcc           <  2:609609/148‑1 (MQ=255)                                                                                                                                           cgcgCCTGGGGCAACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGACCAGCAGaaaa  <  1:228512/149‑1 (MQ=255)                                                                                                                                                cTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGACCAGCAGaaaa  <  2:25647/144‑1 (MQ=255)                                                                                                                                                cTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGACCAGCAGaaaa  >  1:25647/1‑144 (MQ=255)                                                                                                                                                  |                                                                                                                                              TACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGACCAGCAGAAAA  >  NC_000913_3_pae_tpiA/867318‑867604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.