New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 451142541 (1.120)5 (0.140) 4/270 4.6 11.3% coding (906/957 nt) fecD ferric citrate ABC transporter permease
?NC_000913_3_pae_tpiA = 4511432 39 (1.110)coding (899/957 nt) fecD ferric citrate ABC transporter permease
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCG  >  NC_000913_3_pae_tpiA/4511284‑4511570
                                                                                                                                                    |                                                                                                                                          
gATCGTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTTTCCCTGAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCa                                                                                                                                            <  2:95910/149‑1 (MQ=255)
           ttGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCggg                                                                                                                                 >  2:471396/1‑149 (MQ=255)
               cAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCt                                                                                                                             >  1:279078/1‑149 (MQ=255)
                  tGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAGGCAACCCGACAAACCCCGGCGCCCCGATAATGGCGGTCAGCACGCCAACCGGGAGCTccg                                                                                                                          >  1:250326/1‑148 (MQ=255)
                        tGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTgggg                                                                                                                    <  1:232633/149‑1 (MQ=255)
                           aaCGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGAt                                                                                                                 <  2:636662/149‑1 (MQ=255)
                                   tAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAAtt                                                                                                         <  2:279078/149‑1 (MQ=255)
                                   tAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAAtt                                                                                                         <  1:370992/149‑1 (MQ=255)
                                      gTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCAGTCTCACAAGCAACCAGACACACCACGGCGCACCGATAATGGCGGTCGGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATtctc                                                                                                      >  1:275720/1‑149 (MQ=255)
                                                    ccGTAACTGACCGTCAGATTTACAGTTCGTAAAGGAATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCCCGCCCACCGGGAGCCACAGTGGGGGATGACTAATTCTCGCCGGCAGATCGGc                                                                                        >  2:99798/1‑149 (MQ=255)
                                                       tAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAac                                                                                     <  1:211123/149‑1 (MQ=255)
                                                           tGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATtctc                                                                                                      >  2:475334/1‑128 (MQ=255)
                                                           tGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATtctc                                                                                                      <  1:475334/128‑1 (MQ=255)
                                                           tGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCCGACCGCCAgccccc                                                                                 >  2:57963/1‑149 (MQ=255)
                                                                 tCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCaaaaac                                                                           >  1:124513/1‑145 (MQ=255)
                                                                          cAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCt                                                                  <  1:334529/149‑1 (MQ=255)
                                                                            gTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGGGGGGGATGAATAACACTCGCCAGCAGATCGGGCACCACCAACAGCAACGCAcccac                                                                >  2:90839/1‑146 (MQ=255)
                                                                             ttCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCCCCAACAGCAACGCACCTGTc                                                               >  2:593929/1‑149 (MQ=255)
                                                                             ttCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTc                                                               >  1:159290/1‑149 (MQ=255)
                                                                             ttCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACAGGGAGCTCCAGCGGGGGATGAACAAGTCTCGCCAGCAGCTCCGCCACCACCACCACGAACGAACCtctc                                                               >  2:97336/1‑149 (MQ=255)
                                                                                 tAAAGCCATTTATCGCATTCTCACAAGCAACCAGACAACACACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAggg                                                           <  2:474119/149‑1 (MQ=255)
                                                                                      tCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGCCAGGGCCGac                                                      >  2:596125/1‑148 (MQ=255)
                                                                                                cATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGgcagca                                            >  2:184950/1‑149 (MQ=255)
                                                                                                  cgacctcaAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCCCCAGTGGGGCATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGt                                          <  1:296486/143‑1 (MQ=255)
                                                                                                     tcACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTg                                       >  1:586113/1‑149 (MQ=255)
                                                                                                       acaAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCg                                     <  2:159290/149‑1 (MQ=255)
                                                                                                       acaAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCACAAGCTCCAGGGGGGGATGAATAATTCTCGCCAGCAGATCGGCCACCACCAACAGCAACGCACCTGCCAGCGCCCAAACAGGccgcagcccccg                                     >  2:42878/1‑143 (MQ=255)
                                                                                                           gCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCTGAGGTGTAGTAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGa                                 <  1:471396/149‑1 (MQ=255)
                                                                                                              aCCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGt                              >  2:53086/1‑149 (MQ=255)
                                                                                                                cAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAacca                                                                                   <  2:278845/94‑1 (MQ=255)
                                                                                                                cAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAacca                                                                                   >  1:278845/1‑94 (MQ=255)
                                                                                                                     aaaCCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCgg                       <  1:53086/149‑1 (MQ=255)
                                                                                                                              cgcACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTAc              <  1:611635/149‑1 (MQ=255)
                                                                                                                              cgcACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTAc              <  1:323427/149‑1 (MQ=255)
                                                                                                                              cgcACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTAc              <  1:497081/149‑1 (MQ=255)
                                                                                                                                  ccGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCCGGCTGCGGTGACGTCCACCGGTGATGGTACGcat          >  1:94215/1‑149 (MQ=255)
                                                                                                                                   cGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGcctc         >  1:298354/1‑149 (MQ=255)
                                                                                                                                   cGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCCGTGACGACCACCGGCGATGCAACCcatc         >  2:36203/1‑149 (MQ=255)
                                                                                                                                        aTGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATg    <  1:518718/149‑1 (MQ=255)
                                                                                                                                          ggCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCg  <  1:231630/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                          
GATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCG  >  NC_000913_3_pae_tpiA/4511284‑4511570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.