New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 454260616 (0.440)4 (0.120) 4/262 4.5 20.0% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913_3_pae_tpiA = 4542902 17 (0.500)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
Rejected: Coverage evenness skew score above cutoff.

TCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAA  >  NC_000913_3_pae_tpiA/4542774‑4543037
                                                                                                                                |                                                                                                                                       
tCCTCATTCTTCTCTCCAAACACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATACGCTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAATTGtcttgtat                                                                                                                     <  1:219333/149‑8 (MQ=255)
     aTTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCGACAAATGTTTCATCTTTTgg                                                                                                                >  1:209143/1‑149 (MQ=255)
                 aaaaaCCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGACTATTAAGCCAACAAATACACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAACATGCCACCAAATACTTCATCTTTTGGGGGAAAACtgtg                                                                                                    >  2:438792/1‑149 (MQ=255)
                                                         tAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCtttt                                                                                                                  <  1:570766/95‑1 (MQ=255)
                                                         tAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCtttt                                                                                                                  >  2:570766/1‑95 (MQ=255)
                                                             aaTAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAGATGGCCCCAAATGTTTCATCTTTTGGGGGAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAAc                                                       >  1:331289/1‑149 (MQ=255)
                                                                 gAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGAc                                                    >  1:19634/1‑149 (MQ=255)
                                                                          gCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTCCAGAACGACTGCCCATGt                                           >  2:39334/1‑149 (MQ=255)
                                                                                aaaTAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGCCAAAATGGCCCCAAATGTTTCATCTTTTGGAGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACTAAACAAAGTGTACGTGACGACTACCCATGTCGAtgt                                     >  1:124323/1‑147 (MQ=255)
                                                                                 aaTAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTa                                    <  1:482301/149‑1 (MQ=255)
                                                                                            aaaGTTTGCTGGCGATGTTTTCCTCTATGAGTCAAAATGGCCCCATATGTTTTATCTTTTGGGGGAAAACTGTGGAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGtttt                         <  2:308871/149‑1 (MQ=255)
                                                                                                      cgcgATGCTCTCCGTGATGAGTAAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGGCGATTTAGAAATAGTTTTTTgaaaggaa               <  2:54966/149‑1 (MQ=255)
                                                                                                           tGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACtgtg                                                                                                    >  1:369047/1‑59 (MQ=255)
                                                                                                           tGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACtgtg                                                                                                    <  2:369047/59‑1 (MQ=255)
                                                                                                                   tctATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGaaaa  >  1:49829/1‑149 (MQ=255)
                                                                                                                   tctATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGaaaa  <  2:365347/149‑1 (MQ=255)
                                                                                                                    ctATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTgaaaggaaa              <  2:133868/136‑1 (MQ=255)
                                                                                                                    ctATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTgaaaggaaa              >  1:133868/1‑136 (MQ=255)
                                                                                                                                |                                                                                                                                       
TCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAA  >  NC_000913_3_pae_tpiA/4542774‑4543037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.