Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,110,747 G→C intergenic (+62/+784) cdh → / ← yiiQ CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,110,7470GC96.2% 438.9 / 8.5 132intergenic (+62/+784)cdh/yiiQCDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base C (68/58);  total (72/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.17e-01

Only 100 of 136 total aligned reads displayed.

ACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTT  >  NC_000913_3_pae_tpiA/4110608‑4110893
                                                                                                                                           |                                                                                                                                                  
acacAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCcgccgc                                                                                                                                          >  2:706531/1‑150 (MQ=255)
  acaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGa                                                                                                                                        <  1:114538/150‑1 (MQ=255)
  acaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGa                                                                                                                                        <  3:468234/150‑1 (MQ=255)
  acaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAAGAAATTCAGTATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGa                                                                                                                                        <  1:114411/150‑1 (MQ=255)
    aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGATATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGc                                                                                                                                      >  1:584451/1‑150 (MQ=255)
    aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGc                                                                                                                                      <  3:111764/150‑1 (MQ=255)
    aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCACGCCGCGAGc                                                                                                                                      >  4:377675/1‑150 (MQ=255)
    aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGACCCGCCGCGAGc                                                                                                                                      >  1:405429/1‑150 (MQ=255)
        aaaTCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGtt                                                                                                                                  <  1:112317/150‑1 (MQ=255)
        aaaTCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGtt                                                                                                                                  <  1:176758/150‑1 (MQ=255)
           tCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCg                                                                                                                               >  2:695619/1‑150 (MQ=255)
                tGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAAt                                                                                                                          >  4:206254/1‑150 (MQ=255)
                 gACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAAtt                                                                                                                         >  4:449506/1‑150 (MQ=255)
                  aCGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTc                                                                                                                        >  4:34814/1‑150 (MQ=255)
                  aCGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTc                                                                                                                        >  2:4511/1‑150 (MQ=255)
                     cTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGa                                                                                                                                        <  2:722945/131‑1 (MQ=255)
                     cTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTaa                                                                                                                     <  3:413452/150‑1 (MQ=255)
                     cTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTaa                                                                                                                     <  4:352280/150‑1 (MQ=255)
                        aaCCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGAt                                                                                                                  <  3:66733/150‑1 (MQ=255)
                          ccGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCt                                                                                                                >  2:449466/1‑150 (MQ=255)
                           cGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCtt                                                                                                               >  3:405037/1‑150 (MQ=255)
                            gTGCTTCAGCCGAGGAGATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTa                                                                                                              >  3:405908/1‑150 (MQ=255)
                             tGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTaa                                                                                                             <  3:150754/150‑1 (MQ=255)
                             tGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTaa                                                                                                             <  2:26637/150‑1 (MQ=255)
                                    gCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATgg                                                                                                      <  2:584451/150‑1 (MQ=255)
                                     ccGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATggg                                                                                                     >  1:543977/1‑150 (MQ=255)
                                      cGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGTGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGt                                                                                                    <  4:405908/150‑1 (MQ=255)
                                       gAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATTCTGCACCTGGCAGACTTAAGTCCGCTCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTc                                                                                                   >  1:780865/1‑150 (MQ=255)
                                          gAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCttt                                                                                                >  1:755688/1‑150 (MQ=255)
                                          gAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCttt                                                                                                >  2:119330/1‑150 (MQ=255)
                                          gAAATTCAGGATCATCAGTGTGAGACTTCGCGTTAAGGCGAAGAGATAAGCAAAGTAAGTGCCGCATATGACATCCGGCACCCGTCAGACTTAAGGCAGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGCAATTAAGACATGGGTCttt                                                                                                >  2:75775/1‑150 (MQ=255)
                                           aaaTTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCtttt                                                                                               >  1:63081/1‑150 (MQ=255)
                                            aaTTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTg                                                                                              >  2:209302/1‑150 (MQ=255)
                                                 aGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAAtt                                                                                                                         >  4:494626/1‑118 (MQ=255)
                                                 aGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAAtt                                                                                                                         <  3:494626/118‑1 (MQ=255)
                                                   gATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCAc                                                                                       >  1:331652/1‑150 (MQ=255)
                                                           gtgtGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTgcgc                                                                               <  2:390974/150‑1 (MQ=255)
                                                              tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTg                                                                            >  4:474885/1‑150 (MQ=255)
                                                               gagaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGc                                                                           >  1:721718/1‑150 (MQ=255)
                                                               gagaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGc                                                                           <  1:686205/150‑1 (MQ=255)
                                                                            aaGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTccc                                                              <  2:158163/150‑1 (MQ=255)
                                                                             aGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTcccc                                                             >  2:1803/1‑150 (MQ=255)
                                                                               gCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCt                                                                              >  1:324269/1‑131 (MQ=255)
                                                                               gCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCt                                                                              <  2:324269/131‑1 (MQ=255)
                                                                               gCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTc                                                           >  3:513669/1‑150 (MQ=255)
                                                                               gCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAAGTCTAAGATCTGAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTc                                                           >  2:250458/1‑150 (MQ=255)
                                                                                cGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCg                                                          >  4:293516/1‑150 (MQ=255)
                                                                                 gAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGt                                                         >  3:318857/1‑150 (MQ=255)
                                                                                         aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTATGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGtt                                                 <  1:478648/150‑1 (MQ=255)
                                                                                           ggAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGt                                                         >  1:18489/1‑140 (MQ=255)
                                                                                           ggAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGt                                                         <  2:18489/140‑1 (MQ=255)
                                                                                            gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGATTTAAGGCCGCCCCGCCTCTAGCGGTTTCGCCAATGCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGGTTaa                                              >  2:731787/1‑150 (MQ=255)
                                                                                            gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTaa                                              >  1:203187/1‑150 (MQ=255)
                                                                                            gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTaa                                              >  1:712789/1‑150 (MQ=255)
                                                                                            gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCACGCATGACTCCCGTCGTGCCGAGTTTaa                                              >  1:238649/1‑150 (MQ=255)
                                                                                             aaaGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAg                                             >  4:141727/1‑150 (MQ=255)
                                                                                                gtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAgcgc                                          <  1:135978/150‑1 (MQ=255)
                                                                                                 taagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGcgcg                                         >  2:553739/1‑150 (MQ=255)
                                                                                                  aggtGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTgcg                                                                                >  4:213912/3‑110 (MQ=255)
                                                                                                  aggtGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTgcg                                                                                <  3:213912/108‑1 (MQ=255)
                                                                                                  aagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGccgccccgcc                                                                                                                                            <  4:431914/50‑1 (MQ=255)
                                                                                                  aagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGccgccccgcc                                                                                                                                            >  3:431914/1‑50 (MQ=255)
                                                                                                  aagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCgg                                        <  3:34814/150‑1 (MQ=255)
                                                                                                  aagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTCGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCgg                                        >  2:515792/1‑150 (MQ=255)
                                                                                                    gtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGAGATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTACGCGCGGCt                                      <  2:253503/150‑1 (MQ=255)
                                                                                                     tGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCtt                                     >  3:135209/1‑150 (MQ=255)
                                                                                                      gCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTc                                    >  1:82262/1‑150 (MQ=255)
                                                                                                      gCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTc                                    >  2:601527/1‑150 (MQ=255)
                                                                                                      gCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTc                                    <  2:497161/150‑1 (MQ=255)
                                                                                                       ccGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCa                                   >  1:68635/1‑150 (MQ=255)
                                                                                                       ccGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCa                                   <  2:454080/150‑1 (MQ=255)
                                                                                                         ggATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                                 <  2:68635/150‑1 (MQ=255)
                                                                                                               gAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATgg                                                                                                      <  2:122312/75‑1 (MQ=255)
                                                                                                               gAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctc                           >  1:397746/1‑150 (MQ=255)
                                                                                                                aaaTCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctct                          >  2:467201/1‑150 (MQ=255)
                                                                                                                     cGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGct                     >  2:765014/1‑150 (MQ=255)
                                                                                                                      ggCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctc                    <  1:553739/150‑1 (MQ=255)
                                                                                                                      ggCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctc                    >  2:499498/1‑150 (MQ=255)
                                                                                                                      ggCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctc                    <  2:589081/150‑1 (MQ=255)
                                                                                                                      ggCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctc                    >  4:139239/1‑150 (MQ=255)
                                                                                                                       gCACCTTTCAGACTCAAGGCCGCCCCGTCGTGAGCTGTGTCACCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctct                   <  1:75775/150‑1 (MQ=255)
                                                                                                                       gCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctct                   <  3:453332/150‑1 (MQ=255)
                                                                                                                       ccACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGGTATGGGTCTTTTGCCTGCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCTGcgct                   <  2:43737/149‑1 (MQ=255)
                                                                                                                        cACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCtctc                  <  4:187928/150‑1 (MQ=255)
                                                                                                                         aCCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGc                                                                                             >  1:74998/1‑74 (MQ=255)
                                                                                                                         aCCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGc                                                                                             <  2:74998/74‑1 (MQ=255)
                                                                                                                          ccTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCGATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCaa                <  4:368558/150‑1 (MQ=255)
                                                                                                                            tGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGa                                                    >  1:451317/1‑112 (MQ=255)
                                                                                                                              tCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAcc            >  4:315829/1‑150 (MQ=255)
                                                                                                                              tCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGAGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAcc            >  1:652364/1‑150 (MQ=255)
                                                                                                                                 gACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGc         <  1:4511/150‑1 (MQ=255)
                                                                                                                                    ttAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTg                                                        >  2:643117/1‑100 (MQ=255)
                                                                                                                                    ttAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTg                                                        <  1:643117/100‑1 (MQ=255)
                                                                                                                                    ttAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGcccc      >  1:243472/1‑150 (MQ=255)
                                                                                                                                    ttAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGcccc      >  1:719836/1‑150 (MQ=255)
                                                                                                                                      aaGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAccc           <  2:79473/143‑1 (MQ=255)
                                                                                                                                      aaGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAccc           >  1:79473/1‑143 (MQ=255)
                                                                                                                                       aGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTAAACGTCCTCTCCGCTCTCGATGCCCGCCGCAGt   <  2:63081/150‑1 (MQ=255)
                                                                                                                                        ggccgcCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGtt  >  1:40476/1‑150 (MQ=255)
                                                                                                                                        ggccgcCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGtt  >  3:245545/1‑150 (MQ=255)
                                                                                                                                           |                                                                                                                                                  
ACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTT  >  NC_000913_3_pae_tpiA/4110608‑4110893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: