New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1242835	36 (1.070)	6 (0.190)	5/268	3.6	15.0%	coding (246/915 nt)	ldcA	murein tetrapeptide carboxypeptidase; LD‑carboxypeptidase A
	?	NC_000913_3_pae_tpiA	= 1242840	34 (1.070)					coding (241/915 nt)	ldcA	murein tetrapeptide carboxypeptidase; LD‑carboxypeptidase A
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACC  >  NC_000913_3_pae_tpiA/1242695‑1242970                                                                                                                                             |                                                                                                                                        aGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTg                                                                                                                                >  2:321833/1‑150 (MQ=255)  gCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAGTATCTGCCAGTAAACGACTgg                                                                                                                               >  1:89655/1‑150 (MQ=255)    aTGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATGTCTGCCAGTAAACGACTGGCa                                                                                                                             >  1:7230/1‑150 (MQ=255)                   gTGATGACATTGCAATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGACTGCCAGTCACTATATGCCAGTAAACCACGTGCACCGTAACCGCCGCGt                                                                                                              >  2:593417/1‑150 (MQ=255)                      atgaCATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTAca                                                                                                           <  1:149241/150‑1 (MQ=255)                        gaCATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTAcagc                                                                                                         >  1:520036/1‑150 (MQ=255)                          cATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTAcagcca                                                                                                       <  2:520036/150‑1 (MQ=255)                           aTTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTAcagccag                                                                                                      >  1:274948/1‑150 (MQ=255)                                 aTGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGAt                                                                                                >  1:554609/1‑150 (MQ=255)                                       cAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGtt                                                                                          <  2:531577/150‑1 (MQ=255)                                         gaagaGCGCTCTGAATGGCAGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGGGCCTGCAAGTCCATATCTCCTAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTgg                                                                                        <  1:268207/150‑1 (MQ=255)                                            gaCCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTggggg                                                                                     >  1:156324/1‑150 (MQ=255)                                              ccGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTa                                                                                   <  1:233460/150‑1 (MQ=255)                                                  aCTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATCGTTTTggggcaggtca                                                                               >  2:122821/1‑143 (MQ=255)                                                    tGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTggggg                                                                                     >  1:401211/1‑142 (MQ=255)                                                    tGAAGGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTggggg                                                                                     <  2:401211/142‑1 (MQ=255)                                                     aaacggCGGAAAAATCCCCATGTCCGCAAAGGAGCAACGGGTCATGTTGTTTGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTagtcagtc                                                                            <  2:154678/146‑1 (MQ=255)                                                                  aTCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAAtt                                                               >  1:508015/1‑150 (MQ=255)                                                                   tCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAAtt                                                               <  2:436426/149‑1 (MQ=255)                                                                   tCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAAtt                                                               >  1:436426/1‑149 (MQ=255)                                                                    ctcTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTga                                                             <  2:590132/148‑1 (MQ=255)                                                                    cGCAATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTACACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTga                                                             <  1:712/150‑1 (MQ=255)                                                                     gCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAAccgcc                                                                                                                  <  1:426938/96‑1 (MQ=255)                                                                     gCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAAccgcc                                                                                                                  >  2:426938/1‑96 (MQ=255)                                                                      cTATGTCCGCAAAGGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGGGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTgaga                                                           <  2:551603/150‑1 (MQ=255)                                                                            ccGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGAAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAATCTGGCAAGGGAATTGAGATCTTcc                                                     >  2:114601/1‑150 (MQ=255)                                                                                aaaTGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGAc                                                 >  1:416516/1‑150 (MQ=255)                                                                                    gAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGGCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGctc                                             >  2:547496/1‑150 (MQ=255)                                                                                                                  caGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTGCCGTCACCGCAAACAGGTCACAacg               >  1:172955/1‑150 (MQ=255)                                                                                                                    gTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAacgac             <  1:321833/150‑1 (MQ=255)                                                                                                                       cctgccAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACggg          <  1:273341/150‑1 (MQ=255)                                                                                                                       cctgccAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACggg          >  1:46022/1‑150 (MQ=255)                                                                                                                        ctgccAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCCCAAAACGCTCACAACGACGGGc         >  2:526518/1‑150 (MQ=255)                                                                                                                              gtaaacatCTGCCAGTACACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAATACTATTTAGAGCCTCGCGGCTGTCTATTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAAc   <  1:440291/144‑1 (MQ=255)                                                                                                                               tCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAAcc  >  1:337658/1‑150 (MQ=255)                                                                                                                               tCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACAGGCAATAAcc  >  2:490980/1‑150 (MQ=255)                                                                                                                                             |                                                                                                                                        AGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACC  >  NC_000913_3_pae_tpiA/1242695‑1242970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.