New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 281896 =39 (1.160)5 (0.160) 5/266 3.6 11.7% coding (88/1155 nt) yagA CP4‑6 prophage; putative DNA‑binding transcriptional regulator
?NC_000913_3_pae_tpiA 281923 = 39 (1.230)coding (61/1155 nt) yagA CP4‑6 prophage; putative DNA‑binding transcriptional regulator
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCGCGGGCGGTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGT  >  NC_000913_3_pae_tpiA/281747‑282037
                                                                                                                                                     |                                                                                                                                             
ggCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCGCGGGCGGTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGAt                                                                                                                                               >  2:200791/1‑150 (MQ=255)
 gCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCGCGGGCGGTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATg                                                                                                                                              <  2:363711/150‑1 (MQ=255)
      gATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCGCGGGCGGTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGc                                                                                                                                         >  2:439197/1‑150 (MQ=255)
             tcAGATGAGCGGTTCGGGGAATGGTGCGGAATGCGCGGGCGGTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTAGCCCCATcc                                                                                                                                  >  1:460435/1‑150 (MQ=255)
                gATGAGCGGTTCGGGGAATGGTGCGGAATGCGCGGGCGGTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCtcc                                                                                                                               >  1:95111/1‑148 (MQ=255)
                   gAGCGGTTCGGGGAATGGTGCGGAATGCGCGGGCGGTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAg                                                                                                                            >  1:473312/1‑150 (MQ=255)
                     gCGGTTCGGGGAATGGTGCGGAATGCGCGGGCGGTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGc                                                                                                                          <  1:483058/150‑1 (MQ=255)
                             gggAATGGTGCGGAATGCGCGGGCGGTCCTGGAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGaacaaaa                                                                                                                  <  2:480838/150‑1 (MQ=255)
                                    gTGCGGAATGCGCGGGCGGTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTc                                                                                                           >  2:221794/1‑150 (MQ=255)
                                     tGCGGAATGCGCGGGCGGTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTg                                                                                                                                <  1:19621/128‑1 (MQ=255)
                                     tGCGGAATGCGCGGGCGGTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTg                                                                                                                                >  2:19621/1‑128 (MQ=255)
                                             gcgcGGGCGGTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTaa                                                                                                  <  2:485766/150‑1 (MQ=255)
                                              ggcgggcggGGCTGAAGAGCGGCGGCACCTTCCTGAGCCGAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAAt                                                                                                 <  2:511740/149‑1 (MQ=255)
                                                    cggTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGGTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATg                                                                                           <  2:279792/150‑1 (MQ=255)
                                                         cTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTGGGTTGCGGTCGGCGAGGCCACCAAAGCAAACTCAGTAGGTAATGACATGGTAtc                                                                                      >  1:137051/1‑150 (MQ=255)
                                                          tGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATACCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTAtct                                                                                     >  1:512872/1‑150 (MQ=255)
                                                             aGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTAtctctc                                                                                  <  2:434447/150‑1 (MQ=255)
                                                                 cggcggCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCAt                                                                              >  2:146096/1‑150 (MQ=255)
                                                                       cACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAgg                                                                        <  1:439197/150‑1 (MQ=255)
                                                                          cTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTaa                                                                                                  >  1:480655/1‑121 (MQ=255)
                                                                          cTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTaa                                                                                                  <  2:480655/121‑1 (MQ=255)
                                                                           ttCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCAt                                                                    <  1:221794/150‑1 (MQ=255)
                                                                                   cccAGCGATGGAGCCACTTGTAGCCGGTGGCAGGCGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATACCAGGGCCTAGGCGACt                                                            >  2:473109/1‑150 (MQ=255)
                                                                                           tGGAGCCACTTGTAGCCGGTCGGAGGTGAAGTGGCGAAGGGACGGAATAGGGAACGGATGTTCGCTCCGTCCTGCGATGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCCCTCGCATCCCAGGGCATAAGCGACTCCATAAAc                                                    <  2:172184/150‑1 (MQ=255)
                                                                                           gggAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAAc                                                    <  2:404842/149‑1 (MQ=255)
                                                                                                   cTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCACAAGCGACTCCTGAAACGGGTTCtt                                            <  2:460435/150‑1 (MQ=255)
                                                                                                     tGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGCATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTAt                                          <  1:71404/150‑1 (MQ=255)
                                                                                                                  aGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTaa                             >  2:454263/1‑150 (MQ=255)
                                                                                                                      gAAATGCCGAAGCGACGGCAGAGGGAACGGATGTCCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAgtgt                         <  2:97766/150‑1 (MQ=255)
                                                                                                                          tGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTAc                     <  1:146096/150‑1 (MQ=255)
                                                                                                                              gAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGCCATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATg                 >  2:308162/1‑150 (MQ=255)
                                                                                                                                  cGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTccc             >  1:233286/1‑150 (MQ=255)
                                                                                                                                      ggCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGaa         <  2:166071/150‑1 (MQ=255)
                                                                                                                                          gagGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAg     >  1:396683/1‑150 (MQ=255)
                                                                                                                                          gagGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAg     >  2:9446/1‑150 (MQ=255)
                                                                                                                                            gggAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAgtg   <  1:454263/150‑1 (MQ=255)
                                                                                                                                             ggAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAgtgt  >  1:204713/1‑150 (MQ=255)
                                                                                                                                                  ggATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAAc                                                    <  2:390634/95‑1 (MQ=255)
                                                                                                                                                  ggATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAAc                                                    >  1:390634/1‑95 (MQ=255)
                                                                                                                                                     |                                                                                                                                             
GGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGAATGCGCGGGCGGTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGT  >  NC_000913_3_pae_tpiA/281747‑282037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.