Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,111,596 G→A L179L (CTG→TTG)  yiiQ ← DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,111,5960GA95.7% 121.7 / NA 47L179L (CTG→TTG) yiiQDUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (26/18);  minor base C (0/2);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGT  >  NC_000913_3_pae_tpiA/4111459‑4111732
                                                                                                                                         |                                                                                                                                        
aTGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAATCGATTCAGAAAGCGCCAGCTTAAACGGTTCAACAGCGAAGGTCAATCCCTTTTCGc                                                                                                                               >  1:257335/1‑149 (MQ=255)
 tGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGcc                                                                                                                              >  2:77785/1‑149 (MQ=255)
   tccgccCCGCAGGGCAGCAGGTCTGTGAAACAGTATATTGATTCGTCGCCACAAAGGCTTTGCTCTTTGTCATTTATTTAAACCTTCAAGCGATTCAGATTGCGCCAGCTTAATCGGTTCACAAGCGAAGATCAACCCCTTTTAGCCGt                                                                                                                            >  2:141027/2‑149 (MQ=255)
    gcgccCCTCGGGGGAGCAGGTCTTGGAAACAGTATTGAGATTTGTCGGCACAAAGGCTTTGCGTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGAGAGCGCCAGCTTATTCGGTGCAACAGCGAAGGTCAACCCCTTTTCGCCGtt                                                                                                                           <  2:290031/148‑1 (MQ=255)
        cccGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACACAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGCTAGCGCCAGCTTAATCGGTTCAACCGCGAAGGTCAACCCCTTTTCGCCGTTGTcc                                                                                                                       >  1:193212/1‑149 (MQ=255)
          cGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCcgc                                                                                                                     >  1:346531/1‑149 (MQ=255)
          cGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACACCTTTTCGCCGTTGTCcgc                                                                                                                     >  2:47925/1‑149 (MQ=255)
               ggCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGacaa                                                                                                                >  2:37028/1‑149 (MQ=255)
                 cagcagGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCATTGTCCGCGacaaca                                                                                                              >  1:201943/1‑149 (MQ=255)
                          tgtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGt                                                                                                     <  2:346531/149‑1 (MQ=255)
                          tgtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTCCGCCGTTGGCCGCGACAACATAACGCAGt                                                                                                     <  2:72022/149‑1 (MQ=255)
                           gtgAAACAGTATAGAGATTCATCGACACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAAAATAACGCAGTg                                                                                                    >  1:320933/1‑149 (MQ=255)
                            tgAAATAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGAGAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGc                                                                                                   <  2:249386/149‑1 (MQ=255)
                               aaCAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTgaagc                                                                                                >  1:182456/1‑145 (MQ=255)
                                   gTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCt                                                                                            <  1:37028/149‑1 (MQ=255)
                                           aTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGgt                                                                                    <  1:112894/149‑1 (MQ=255)
                                            ttCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGgtg                                                                                   >  1:325672/1‑149 (MQ=255)
                                               aTCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGtaa                                                                                <  1:213042/149‑1 (MQ=255)
                                                tCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGtaat                                                                               >  1:563789/1‑149 (MQ=255)
                                                   gCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAAc                                                                            <  1:45320/149‑1 (MQ=255)
                                                        aaGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTTAACAGCGACGGTCAACCCCTTTTCGCCGTTGTCCGCGACGACATAAAGCCGTGCACCCTCTGTCTCGGTGGAATAACgtttg                                                                       >  1:284193/1‑149 (MQ=255)
                                                            ctttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCCGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATCACGTTTGtttt                                                                   >  1:28730/1‑149 (MQ=255)
                                                                     ttGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCccgccg                                                          >  1:562633/1‑149 (MQ=255)
                                                                       gTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGtt                                                        >  2:404135/1‑149 (MQ=255)
                                                                        tCATTTATTGGAACCTTCAAGCGTTTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTAGCCGTTGTCCGCTGCAACATCACGCTGTGCACCTTCTGTCTCGGTATAATATCGTTTGCTTTTCCCCCTCGttg                                                       >  2:216318/1‑148 (MQ=255)
                                                                        tCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGt                                                                                                     <  1:411553/103‑1 (MQ=255)
                                                                        tCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGt                                                                                                     >  2:411553/1‑103 (MQ=255)
                                                                               ttCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTAAAACCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGa                                                >  1:431310/1‑149 (MQ=255)
                                                                                  aaaCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCAGTAGAATAACGTTTGCTTTTCACAGCCGTTTGTGCCGACta                                             >  2:119827/1‑148 (MQ=255)
                                                                                       ttaaaGCGATGCAGATAGCGCCACCTAAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtt                                        <  2:562633/146‑1 (MQ=255)
                                                                                            gCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtttttt                                    <  2:413249/148‑1 (MQ=255)
                                                                                            gCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtttttt                                    >  1:413249/1‑148 (MQ=255)
                                                                                              gATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTg                                 >  2:71421/1‑149 (MQ=255)
                                                                                                   gtagaGCGGCAGGTTGATCCGTTCAACAGCGAGTGTCACCCTCTTTTCGCCGTTGTACCCAACAACATAACGCGGTGCACCTTCTGTCTCCGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCttt                            <  2:280636/145‑1 (MQ=255)
                                                                                                   aGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCa                                           <  1:342082/134‑1 (MQ=255)
                                                                                                   aGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCa                                           >  2:342082/1‑134 (MQ=255)
                                                                                                        gcgcCAGCTTAATGGGTTCCACAGGCAAGATCACTCCCTTTTCGGTGTTGTCTGCGACAATATAACGCAGTGCGCCTGCTGTCTCGGTGTAATTACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTGTTTCTGGCTTTGTGtt                       <  2:43817/149‑1 (MQ=255)
                                                                                                         cgcCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGttt                      <  1:379641/149‑1 (MQ=255)
                                                                                                               cTTAATCGGTTCAACAGCGCAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAAAGTTTGTTGTTCCCCGCCGTTAGTGGCGATTGCGGTTTTTTCTGGCTTTGTGTTCTGGTcc                >  2:205773/1‑148 (MQ=255)
                                                                                                                  aaTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTa             >  1:184685/1‑149 (MQ=255)
                                                                                                                       gTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggg        <  1:77785/149‑1 (MQ=255)
                                                                                                                          cAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTCTTGGTCATTAATGGGGTg     >  2:609049/1‑149 (MQ=255)
                                                                                                                          cAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGGGTTTTTGTCATTAATtggggg     >  1:71426/1‑147 (MQ=255)
                                                                                                                           aaCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGa    <  1:19722/149‑1 (MQ=255)
                                                                                                                             caccGAACGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAgt  <  1:311847/146‑1 (MQ=255)
                                                                                                                             cAGCGAAGGTCAACCCCTTTTCGCTGTTGGCGGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAgt  <  2:563789/149‑1 (MQ=255)
                                                                                                                             cAGCAAAGGTCAACCCGTTTCCGGCGTTTACGGCTACAACATAACGCAGTTCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGAGTGCgt  <  2:320933/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
ATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGT  >  NC_000913_3_pae_tpiA/4111459‑4111732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑