Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	264,098	0	G	T	100.0%	109.2 / NA	36	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/17);  total (19/17)

TCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGC‑CCTGCGTTTACTGATAATTT  >  NC_000913_3_pae_tpiA/263957‑264243                                                                                                                                              |                                                                                                                                                   tCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTc                                                                                                                                             >  1:617141/1‑149 (MQ=255)   ccAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCg                                                                                                                                           >  1:298394/1‑149 (MQ=255)     aGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGc                                                                                                                                         >  1:522547/1‑149 (MQ=255)                aaTCTGCCGCTGCAGGCTGCTGGTAGGGTTGCTCATTACAGTACTCCACTATTGGCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGaa                                                                                                                              <  2:65927/149‑1 (MQ=255)                  tctcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACg                                                                                                                            >  2:530571/1‑149 (MQ=255)                   ctcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt                                                                                                                           >  2:361808/1‑149 (MQ=255)                    tcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTc                                                                                                                          >  1:478502/1‑149 (MQ=255)                      ccGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGTCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTccc                                                                                                                        >  1:142144/1‑149 (MQ=255)                            cgtggcccTGGTAGGGTTGCTCATTACAGTTCGCGACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGCCTCCGGCGATATCCGGAACGTCCCTGCCgg                                                                                                                  <  2:527876/144‑1 (MQ=37)                              ggCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                                                                                                                <  1:167405/149‑1 (MQ=255)                               gCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtgt                                                                                                               >  1:29741/1‑149 (MQ=255)                               gCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtgt                                                                                                               >  1:5394/1‑149 (MQ=255)                                     ggtggtgTTGCTCATTACAGTTCTCCACTTGTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGTGCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGAc                                                                                                         <  2:409858/143‑1 (MQ=37)                                     ggTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCCGTGTCATGAc                                                                                                         >  2:282710/1‑149 (MQ=255)                                           gTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGCATAGGGATCTCTGCGTTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCGGGTGTAATGACAGACGc                                                                                                   >  1:118451/1‑149 (MQ=255)                                                 taatACAGTTCCCCACTATTGTCAGTTAAGGGTGATGGTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGt                                                                                             <  2:5394/146‑1 (MQ=25)                                                              cACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGCAATGACAGACGCTGCCGTGCCCCTGGTAttt                                                                                >  2:289736/1‑149 (MQ=255)                                                                cTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGc                                                                              >  1:557557/1‑149 (MQ=255)                                                                                  gATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTACACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGa                                                            <  2:298394/149‑1 (MQ=255)                                                                                       tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCgg                                                       <  2:29741/149‑1 (MQ=255)                                                                                       tcatcaCGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAACGACAGACGCTACCGTGCCACTGGTATTTGCCGGTACAGTAGCGAAAGATTCgg                                                       <  1:408426/149‑1 (MQ=255)                                                                                        catcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGCCGTTCGGCGTATACCTCTAGGACTCCGGCGATATCCGGAACGTGCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGAGCAGTGACGAAAGATGCggg                                                      >  2:582136/1‑149 (MQ=21)                                                                                                     taGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTg                                         <  2:617141/149‑1 (MQ=255)                                                                                                     taGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTg                                         <  2:142144/149‑1 (MQ=255)                                                                                                     caGGGCCCATCGCGGTCCTGGCGGCGGTCGGCGGATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTTTAATGACATACTGTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTg                                         <  1:196975/148‑1 (MQ=18)                                                                                                        ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCg                                      <  1:136781/149‑1 (MQ=255)                                                                                                          cccATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGt                                    >  1:37669/1‑149 (MQ=255)                                                                                                            cATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGtat                                  >  1:7920/1‑149 (MQ=255)                                                                                                             aTTGCGGCCCCGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGtata                                 <  1:479716/149‑1 (MQ=255)                                                                                                                        ggcggcGGTCGGCGTATACCGCTAGGCCTCCGGCGATATCGGGAACGGCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGTCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGc‑c                     <  1:282710/149‑1 (MQ=17)                                                                                                                               ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGc                                                                              >  1:606058/1‑86 (MQ=255)                                                                                                                               ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGc                                                                              <  2:606058/86‑1 (MQ=255)                                                                                                                               ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGa                                                     <  2:204564/111‑1 (MQ=255)                                                                                                                               ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGa                                                     >  1:204564/1‑111 (MQ=255)                                                                                                                                  ggCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGC‑CCTGCGTTTAc           <  2:478502/149‑1 (MQ=255)                                                                                                                                      tataCCGCTCGGACTCCGGCGATATCCGGAAAGTCCCTGCCAGTGTAATGACGGACGCTGCCGTGCCCATGGGAATTGCGGGTGCAGGAGGGAAAGATACAGCACTAAGGATGCTGGCGGTAGAACGTCATTGCACGTGCGttatttcg        >  2:42705/1‑143 (MQ=255)                                                                                                                                           cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGC‑CCTGCGTTTACTGATAAttt  >  2:60563/1‑149 (MQ=255)                                                                                                                                           cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGC‑CCTGCGTTTACTGATAAttt  >  1:260611/1‑149 (MQ=255)                                                                                                                                           cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAACTTCGGGACTCAAGATACTGGCGGTATCTCGGCATTGC‑CCTACGTATCCTGATaaata  >  2:605553/1‑146 (MQ=14)                                                                                                                                              |                                                                                                                                                   TCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGC‑CCTGCGTTTACTGATAATTT  >  NC_000913_3_pae_tpiA/263957‑264243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑