Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	275,065	G→A	T18I (ACC→ATC)	insH1 ←	IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	275,065	0	G	A	100.0%	38.9 / NA	17	T18I (ACC→ATC)	insH1	IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/8);  total (9/8)

CAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAA  >  NC_000913_3_pae_tpiA/275007‑275210                                                           |                                                                                                                                                  cAAGAAAATCTCTTTTTTGGTCTGGCGTGGCTGATTGGTGAATTCAGTGTCGGCGGAGATAAGTTGATGATTCATGATGAACCCTGTTCTATTGCTCCAGTTGACAAACATGTTCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGc                                                         <  2:31911/149‑1 (MQ=11) aaaGAAAATCTCTTTGCTGGTCTGAAGGCGCTTACTGCTGAATTCACCTTCGGAGAAGATAAGTTGATGACGCATGATGAGCGCCGTTCGATGGGTCCAGATGAGAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGc                                                         <  2:147482/148‑1 (MQ=11)  aaGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCg                                                        >  2:421098/1‑149 (MQ=25)    gAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGACGAAGATAAGTTGATGGCTCATGATGAACCCTGTTATATGGCCCCAGATGACAAACATGATCTGCTATCAGGGAATTGTTAGCAACTTGAGGAGGCgat                                                      >  1:129210/1‑147 (MQ=11)      aaaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTAt                                                    <  2:316125/149‑1 (MQ=255)        tcctcgTTTGTGGTTGGGCGGCGCTTTCTGCTGAATGTGCTTTCGGCGAAGATAATTTGATGACTCATTATGAAACCTGTTCTATGTCTTCTGATGACAAACATGATCTCATATTAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGa                                                  <  2:488557/147‑1 (MQ=14)          ctctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGCCTTGTTCGCACCTTCCGGCGGAGTTATgcgc                                                >  2:48853/1‑149 (MQ=11)                 cgggTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTGCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGTGGCGTTATGAGGTggcggc                                         >  2:524488/3‑149 (MQ=14)                 cTGGTCTGACGGCGCCTACTGCTCAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTAAGAGCTggcggc                                         <  1:381467/149‑1 (MQ=255)                  tGGTCTGACGGCGCTTACTGGTGAATGCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGcc                                        <  2:440027/149‑1 (MQ=255)                         aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTg                                 >  1:37053/1‑149 (MQ=255)                         aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTg                                 >  1:615195/1‑149 (MQ=255)                                     gctgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGattatt                     >  1:197845/1‑149 (MQ=255)                                      ctgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTACTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTAGCGGCCCTTTTTGTATCTGAttatta                    >  1:81902/1‑149 (MQ=255)                                           ttCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATccc               <  1:421098/149‑1 (MQ=255)                                                     gCGATGATAAGTTTATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTAt     >  2:263074/1‑149 (MQ=255)                                                        aaGATAATTTTATGACTCATGATGAACCCTGTTTTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGTACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTaa  <  2:79619/149‑1 (MQ=25)                                                           |                                                                                                                                                  CAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAA  >  NC_000913_3_pae_tpiA/275007‑275210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑