New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 4322620 =32 (0.890)5 (0.140) 5/276 4.3 12.8% pseudogene (111/372 nt) phnE defective phosphonate ABC transporter permease;transport; Transport of small molecules: Anions; membrane channel protein component of Pn transporter; membrane component of ABC superfamily
?NC_000913_3_pae_tpiA 4322653 = 37 (1.050)pseudogene (78/372 nt) phnE defective phosphonate ABC transporter permease;transport; Transport of small molecules: Anions; membrane channel protein component of Pn transporter; membrane component of ABC superfamily
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGC  >  NC_000913_3_pae_tpiA/4322523‑4322801
                                                                                                                                  |                                                                                                                                                    
ccGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCAcc                                                                                                                                    >  2:366368/1‑149 (MQ=255)
          gTTGGATTGGATGCGATAGAGGGGTTGGCGGATCCGCAGTGCCATCGCCTGTGGCGGTTCGCCGTAGACGATCTCTCCGAGCTTGTCGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAAGCGCTTCCACCGCTTCGGaaa                                                                                                                          <  2:324971/149‑1 (MQ=255)
                   gAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTgg                                                                                                                 >  1:289742/1‑149 (MQ=255)
                    aaGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCGCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGga                                                                                                                >  1:341060/1‑149 (MQ=255)
                            ggtggAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGc                                                                                                        <  2:307447/146‑1 (MQ=255)
                                     ggAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGgtgt                                                                                               <  1:366368/149‑1 (MQ=255)
                                     ggAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTAGACGCCACGCCGgtcgtgc                                                                                               >  2:565191/1‑148 (MQ=255)
                                        gaTCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAACAGCTGGGCGACCACGCCGGTGGTGTGGa                                                                                            >  2:373247/1‑149 (MQ=255)
                                         aTCAGCAGTGCCACCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGAt                                                                                           >  2:77151/1‑149 (MQ=255)
                                             gcagGGGCATCACCTGTGGCAGCAGGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAAc                                                                                       <  2:605056/149‑1 (MQ=255)
                                              cagTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACa                                                                                      <  1:361234/149‑1 (MQ=255)
                                                  ggCATCACCTGGGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAgcgc                                                                                  <  2:467047/149‑1 (MQ=255)
                                                      tCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGcgccagc                                                                              >  1:340498/1‑149 (MQ=255)
                                                        ccctgcagcagcaCGCCGTAGAGGATCTCTCCGAGCTTGTTGGTACCGGTGGCGAGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCGTCGGAAAGTAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGcgccagcac                                                                            <  1:416039/142‑1 (MQ=255)
                                                        cccTGTGGCAGCACGCCGGAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGcgccagcac                                                                            <  2:341060/148‑1 (MQ=255)
                                                          cTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCCCTTCGGCAAGCAGCTTGGGGAGCACGCCGGTGGTGCGGATCAACAGccccagcacgc                                                                          >  2:248428/1‑149 (MQ=255)
                                                          cTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTCCCATCTGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGGGCACGCCGGGGGTGTGGATAAACAGcgccagcacgc                                                                          >  1:91047/1‑149 (MQ=255)
                                                             tgGCAGCACGCCGTAGAGGATCTCTGCGAGCGTGGTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGcgccagcacgccag                                                                       <  2:145192/149‑1 (MQ=255)
                                                                    ccGCCGTAGAGGACCTCTTCGAGCTTGTTGGCACCGGGGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAgcacgccagcacgcca                                                                <  1:136887/148‑1 (MQ=255)
                                                                            gagGATCCTTGCGAGCGTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGCAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACgg                                                        <  2:265886/149‑1 (MQ=255)
                                                                                   tctTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGggccgagg                                                 >  1:81647/1‑149 (MQ=255)
                                                                                     ttCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGACCGggccgaggcc                                               >  2:374888/1‑149 (MQ=255)
                                                                                     ttCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAACCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGggccgaggcc                                               >  1:419659/1‑149 (MQ=255)
                                                                                        gAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAc                                            >  1:412832/1‑149 (MQ=255)
                                                                                           cTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCAACGCTTAGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCGGCACGCCAGCACGCCAGCGAAGGGGCCGAGGCCGACGGc                                         >  1:374804/1‑149 (MQ=255)
                                                                                              gttgGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGAc                                      <  2:412832/149‑1 (MQ=255)
                                                                                               ttgGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTAAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGAcc                                     <  1:176204/149‑1 (MQ=255)
                                                                                                      cGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACa                              >  1:92895/1‑149 (MQ=255)
                                                                                                         tGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCa                           <  2:340498/149‑1 (MQ=255)
                                                                                                            cgcgAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATgg                        <  1:522317/149‑1 (MQ=255)
                                                                                                              gaaaTGCTTGCCACCGGGCCGGGCTCAATCGCTTCCACCGGTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCg                      <  2:124380/147‑1 (MQ=255)
                                                                                                                 aggCCTTCACCCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAg                   <  2:89553/147‑1 (MQ=255)
                                                                                                                     cTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGGGACGGCCa               >  2:197435/1‑149 (MQ=255)
                                                                                                                      ttCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGa                                                                                            <  2:566578/71‑1 (MQ=255)
                                                                                                                      ttCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGa                                                                                            <  2:566587/71‑1 (MQ=255)
                                                                                                                      ttCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGa                                                                                            >  1:566587/1‑71 (MQ=255)
                                                                                                                      ttCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGa                                                                                            >  1:566578/1‑71 (MQ=255)
                                                                                                                               gccgggcTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAAt     <  2:417999/149‑1 (MQ=255)
                                                                                                                                 cgggcTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATgg   >  1:409553/1‑149 (MQ=255)
                                                                                                                                  gggcTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACCGCATGGGGAAGACCCGTTCGTTAATGgc  >  2:334976/1‑149 (MQ=255)
                                                                                                                                  |                                                                                                                                                    
CCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGC  >  NC_000913_3_pae_tpiA/4322523‑4322801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.