| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 1,197,502 | C→T | G11R (GGA→AGA) | ymfD ← | e14 prophage; putative SAM‑dependent methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 1,197,502 | 0 | C | T | 96.0% | 68.2 / NA | 25 | G11R (GGA→AGA) | ymfD | e14 prophage; putative SAM‑dependent methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (11/13); minor base A (1/0); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATAC > NC_000913_3_pae_tpiA/1197357‑1197636 | cagATTAATTCATCAGAAGATCTAAGTTGTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATAGCCTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCtgtg < 2:33132/147‑1 (MQ=255) tGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCATTGCTTCTAATATACTCGCAAAGATATCTAAAAGCCATCGTATGAGATTTTTCTGCATTCTCTCAGCACATATTAACTCTGTGc > 2:400011/1‑149 (MQ=255) ttCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATAATCGCAAAGATATCTAGAGGGAATCGTATGAGCTTTTGCTGCATTCTATGAGCTACTTTTAACTCTGTGCAttttat > 2:501271/1‑149 (MQ=255) gAATAACTAAGTTTTCCGGAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCaa < 2:421469/149‑1 (MQ=255) aaTATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCaaa < 2:120985/149‑1 (MQ=255) tAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGt > 1:270589/1‑149 (MQ=255) aaGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGAAATCTAGAGGGCATCGTAAGAGGTTTTGCTGCATTATCTAAGCGAATATTAACACAGTGAATAATCTAATTCACAGCAAGTa > 2:305615/1‑149 (MQ=255) ttCCGCAGCCAAAAGCAAAGGCGTGTCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGCATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCAtt < 1:12248/149‑1 (MQ=255) cGCAGCCAAAATCAAGGGCGTGGCCATTGTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCaa < 2:379330/149‑1 (MQ=255) cGCAGCCAAAATCAAGGGCGTGGCCATTGTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCaa < 1:287298/149‑1 (MQ=255) cATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAAt > 1:455001/1‑149 (MQ=255) ttCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCttt > 1:458932/1‑149 (MQ=255) cgtCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATc < 2:337822/147‑1 (MQ=255) tAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTcc > 1:134481/1‑149 (MQ=255) cggaacgATATCTAGAGGGCATTGTATGAGGTTTTGCTGCATTGTCTGAGCGAATATTAACTCTGTGCAAATTATAGTTCAAAGCAAGTACCATTCTATTAATGTTATTTTTTGTTAAAAATTCTTTTATTTTATCGTCCGGGAttttt < 2:234476/143‑1 (MQ=255) cttATGAGTTTTTGCTGCATTGCCTGAGCGAATATTAACTCTGTGCATATCATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTCAGTGAAAAATTCTTTTACTGTATCGGCCCGGAGTTTTCCGACTTGACTTTCAGTTCTgg < 2:590850/147‑1 (MQ=255) cGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTgg < 1:270390/149‑1 (MQ=255) cGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCACATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATGTTTATTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTgg < 1:374632/149‑1 (MQ=255) tGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCTTACGGGATTTTTCTGACTTGACTTTCAGTTCTGGGCtt > 1:15656/1‑149 (MQ=255) gTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCTTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACt > 1:381670/1‑149 (MQ=255) ctgcATTCTCTGAGCGGATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTGCCGACGTGACTTTCAGTTCTGGGCTTAACTGTcaaa < 2:613710/149‑1 (MQ=255) tgcATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGCTATTTTTAGTGAAAAAGTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTCTCAGTTCTGGGCTTAACTGTcaaag > 1:573929/1‑149 (MQ=255) tctGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTc < 2:194357/138‑1 (MQ=255) tctGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTc > 1:194357/1‑138 (MQ=255) tctGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGat > 2:115497/1‑149 (MQ=255) gCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATAc < 1:115497/149‑1 (MQ=255) | CTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATAC > NC_000913_3_pae_tpiA/1197357‑1197636 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |