| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 4,296,296:1 | +CG | 100% | intergenic (+586/+56) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 4,296,296 | 1 | . | C | 90.0% | 37.3 / 0.8 | 20 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (5/13); total (6/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_000913_3_pae_tpiA | 4,296,296 | 2 | . | G | 90.0% | 38.3 / 0.6 | 20 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (5/13); total (6/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑‑A‑‑C‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGC > NC_000913_3_pae_tpiA/4296212‑4296430 || gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑‑ACGC‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCGGGCCTATCTTAACCg > 1:273059/1‑149 (MQ=255) cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑‑ACGC‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAt < 1:734247/149‑1 (MQ=255) tCTTATCAGGGCGACGCCAGACAGCGCAATAGCCTGACTTAGCGTGATTTTGTAGGGCGGATAAGGCGTT‑T‑‑ACGC‑CCCCTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttttt < 2:558729/149‑1 (MQ=14) aTCAGGCCTACGCCAGAGAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑‑ACGC‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa < 1:162004/149‑1 (MQ=255) gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTAGGGTGCTTTGTT‑T‑‑ACGC‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt > 1:695713/1‑149 (MQ=17) cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑‑ACGC‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCacac < 1:670923/149‑1 (MQ=255) ggaaTTGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑‑ACGC‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa < 2:71602/147‑1 (MQ=18) gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑‑ACGC‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa < 1:467432/149‑1 (MQ=255) gcAATACCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGCT‑T‑‑ACGC‑CGCATCCCCCACCAATGCCTGATGCGACGCTTAACTCCAGTTGGCAGGTCTATCTAAGCCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa < 1:287392/149‑1 (MQ=12) aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑‑ACGC‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGATGGTTAATTCTTCAAACTCTTCACACCCGGTATCAAAcc > 1:569338/1‑149 (MQ=18) aaTAGCCTGATTTAGCGTGATTAAATAGGGCGGGTGGGGTGTTGT‑‑G‑‑CTTGCTGCCCGCAGAAATGCATGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc < 1:164550/149‑1 (MQ=19) tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑‑ACGC‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCt < 1:214239/149‑1 (MQ=255) gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑‑ACGC‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc < 1:75549/149‑1 (MQ=255) cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑‑ACGC‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACGCCCGGTATCAAACCCTTCCa > 1:144092/1‑149 (MQ=255) tAGAGTGAGTTTGTAGGGTGGATAAGGCGTG‑T‑‑ACGC‑CGCATCCGAGATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTCTCAAACCCTTCCATACAGc < 2:622809/149‑1 (MQ=21) cGTGATTTTGTAGGTCGGATAAGGCGTT‑TGCG‑‑C‑CGCATCCGACATCAATGCCTGCTGAGACACTTGCCGCTTCTTATCAGGCCTATCTTAACCGTTGGTTAATGTTTCAAACTCTTCACACCCGGGCTCAAACCCTTCCATACAACTCa > 1:620633/1‑149 (MQ=21) gTGATTTTGTAGGGGGGATAAGGCGTT‑T‑‑ACGC‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAg < 2:532385/149‑1 (MQ=25) gTGATTTTGTAAGTCGGATAAGGCGTT‑T‑‑ACGC‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAg < 1:383625/149‑1 (MQ=255) tGATTTTGTAGGTCGGATAAGGCGTT‑T‑‑ACGC‑CGCATCCGACCTACTTTTGAGAAGCAAAGGTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAgg > 1:541651/1‑149 (MQ=25) gATAAGGCGTT‑T‑‑ACGC‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgc < 1:329418/149‑1 (MQ=255) || GACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑‑A‑‑C‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGC > NC_000913_3_pae_tpiA/4296212‑4296430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |