Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913_3_pae_tpiA 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 257908 258683 776 44 [0] [1] 45 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 2579070 (0.000)39 (1.060) 30/260 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913_3_pae_tpiA 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

GCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913_3_pae_tpiA/257761‑258055
                                                                                                                                                  |                                                                                                                                                    
gCTTGTGTTAAAATTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCCCTAGGCCCGTATATTCGTGAAGGTAag                                                                                                                                                    <  1:473652‑M1/149‑3 (MQ=255)
  ttGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtg                                                                                                                                                  >  1:448710‑M1/1‑145 (MQ=255)
   tgtgtgAAAAGTTTGCTAAATTTTGCCAATTTGGTAAAACCGGTGAATCAACACAGGAGATAGCAATGACGGTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTGTCCGCACTCTGCCCTTATATTCGTGAAGGTAagtgc                                                                                                                                                 <  2:371157‑M1/149‑6 (MQ=255)
            aagttgctAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCACGTATATTCGTGAAGGTAagtgcaaagataat                                                                                                                                        <  2:598314‑M1/146‑15 (MQ=255)
               ttGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcga                                                                                                                                     >  1:412642‑M1/1‑132 (MQ=255)
                          tGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGAAACCCGAAGAGTAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcga                                                                                                                          <  2:446501‑M1/149‑29 (MQ=255)
                          tGCCAATTTGGTAAAACAGTTGCATAACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcga                                                                                                                          <  1:663926‑M1/149‑29 (MQ=255)
                            ccAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgatt                                                                                                                        <  1:479444‑M1/149‑31 (MQ=255)
                              aaTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTTGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgt                                                                                                                      >  1:481474‑M1/1‑117 (MQ=255)
                              aaTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgt                                                                                                                      >  1:644721‑M1/1‑117 (MQ=255)
                                            gTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagattttcgattctttttcgattgtctggctgtatgcgt                                                                                                        <  2:710914‑M1/149‑47 (MQ=255)
                                                    acaacaGGAGATAGCAATGACGTTACCGAGTGGACCCACGCAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtga                                                                                                >  1:658338‑M1/1‑95 (MQ=255)
                                                      aacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaa                                                                                              >  1:432473‑M1/1‑93 (MQ=255)
                                                       acaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcggcaacgtgaaac                                                                                             >  2:558327‑M1/1‑92 (MQ=255)
                                                                  cAATGACGTTACCGAGTGGACACCCGAACACCATACTTCGGAGTAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaa                                                                                  <  1:570160‑M1/149‑69 (MQ=255)
                                                                  cAACGACGTTTCCGAGTGGACACCAGAAGAGCAGATTGATCAAAACATCTACCGAACTAGGCCCGGATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaa                                                                                  <  1:151301‑M1/149‑69 (MQ=255)
                                                                     tGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtg                                                                               >  1:666760‑M1/1‑78 (MQ=255)
                                                                      tacGTTACCGAGTGGAAACCCGAAGAGCAGATTGATCAAAAAATTTACCGCTCTAGGCCCTTATATTCGTGAAGGTAagtgcaaagataaacgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgc                                                                              <  1:458575‑M1/148‑73 (MQ=255)
                                                                              cGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTCCCGCTCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattc                                                                      >  1:728176‑M1/1‑69 (MQ=255)
                                                                                       acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctgg                                                             >  1:607286‑M1/1‑60 (MQ=255)
                                                                                         ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttgtattttctgtctgtatgcgtcaacatgaaaccgacaccggcagtgcgtgaattctggagctggtg                                                           >  2:578336‑M1/1‑58 (MQ=255)
                                                                                         ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattttctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgacttctggggctggtg                                                           >  2:723392‑M1/1‑58 (MQ=255)
                                                                                         ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtg                                                           >  1:732505‑M1/1‑58 (MQ=255)
                                                                                         ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctgggg                                                           >  1:48196‑M1/1‑58 (MQ=255)
                                                                                           gAAGAGCAGATTGATCAAAAAATTTCCCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttttgattgtctggctgtatgcttcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgga                                                         <  2:127910‑M1/149‑94 (MQ=255)
                                                                                            aaGAGCAGATTGATCAAAAAATTTACCGCACTACGCCCGTACATTCGTGAAGGTAagtgcaaagatcatggattcttttttgattttcgtgctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggat                                                        <  1:540753‑M1/149‑95 (MQ=255)
                                                                                             agagCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgcctggctgtattcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatg                                                       <  1:140954‑M1/149‑96 (MQ=255)
                                                                                             agagCAGATTGATCAAAAAATTTACCGCACTAGGCCCGGATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatg                                                       <  1:273570‑M1/149‑96 (MQ=255)
                                                                                             agagCAGAGTGATCAAAAAATTTACCGCACTAGGCCCGTATATGCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatg                                                       <  2:611927‑M1/149‑96 (MQ=255)
                                                                                               agCAGATTTATCAAAAAATTTACCCCACCAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtatggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatgga                                                     <  1:639848‑M1/149‑98 (MQ=255)
                                                                                                gCAGAGTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggag                                                    <  1:91601‑M1/149‑99 (MQ=255)
                                                                                                    aTTGATCAAAAAATTTCCCGCACTTGGCCGGTATGGTCGTGAGTGTAagtgcaaagataatcgattctttttcgattgtttggctgtatgcgtcaacgtgaagccggcaccggtagtgcgtgaattctggggctggtggatggagctgg                                                <  2:164907‑M1/149‑103 (MQ=255)
                                                                                                        aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagc                                            <  1:433887‑M1/149‑107 (MQ=255)
                                                                                                         gcaAAAAATTTACCGCACTGGGCCCGTATATTCGTGAAGGCAagtgcaaagataatcgattctttttcgattgtctggtggtatgcgtcaacgtgagaccggcaccagaattgcgtgaattctggggctggtggatggagctggaagcg                                           >  2:552708‑M1/2‑42 (MQ=255)
                                                                                                           aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         <  1:129802‑M1/149‑110 (MQ=255)
                                                                                                              aaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagcgggaagggcagga                                      >  1:156060‑M1/1‑37 (MQ=255)
                                                                                                                  ctaCCGCGCTAGGCCCGTAGATTCGGGAAGGTAagttcaaagataatcgagtctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcc                                  <  2:412642‑M1/148‑117 (MQ=255)
                                                                                                                     ccGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgt                               <  1:395989‑M1/149‑120 (MQ=255)
                                                                                                                          cTAGGCCCGTATACTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttac                          <  1:715863‑M1/149‑125 (MQ=255)
                                                                                                                              gCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctac                      <  1:218586‑M1/149‑129 (MQ=255)
                                                                                                                                    atatTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgcattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttac                >  1:464906‑M1/1‑15 (MQ=255)
                                                                                                                                    atatTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttac                <  1:185395‑M1/149‑135 (MQ=255)
                                                                                                                                    acattcGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtaagcgtcaacgtgaaaccggcaccggaagtgcgtgacccctgatacggtgggatggagcgggaagcgcaggaataccgttttacctacagttac                >  1:465082‑M1/3‑15 (MQ=255)
                                                                                                                                                  aagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgtt  >  1:401079‑M1/1‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913_3_pae_tpiA/257761‑258055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.