Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 1,197,502 C→T 100% G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA1,197,5020CT100.0% 112.7 / ‑6.7 40G11R (GGA→AGA) ymfDe14 prophage; putative SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (27/12);  total (28/12)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCAC  >  NC_000913_3_pae_tpiA/1197356‑1197643
                                                                                                                                                  |                                                                                                                                             
acttaatAAGTCAGCAGAATATCTAAGTTTTCCGCATCCAAAATCAAGGGCGTGGCCATTTTTCTCAATTCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATCAGGTTTTTCTGCATTCTCTGAGCGAATATTAACTCTGt                                                                                                                                             <  2:123144/143‑1 (MQ=255)
  tGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGc                                                                                                                                           >  1:495387/1‑149 (MQ=255)
    aTTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATGGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGctt                                                                                                                                         >  1:118329/1‑147 (MQ=255)
    aTTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGACATCTTATGAGGTTTTGCTGCATTCTCTGAGCGACTATTAATTCTGTGcct                                                                                                                                         >  2:548234/1‑147 (MQ=255)
        aTTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTATGTGCAtatta                                                                                                                                     >  1:322708/1‑149 (MQ=255)
           catcaGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAg                                                                                                                                  >  2:439379/1‑149 (MQ=255)
            atcaGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATATTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTATGTGCATATTATAGt                                                                                                                                 >  2:602727/1‑149 (MQ=255)
             tcaGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACACGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAAAATTAACTCTGTGCATATTATAGtt                                                                                                                                >  1:158636/1‑149 (MQ=255)
                gAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCTTTTTTCTCAATTCTTCTAATATACTCGCAAAGAGATCTAGAGGGCATCGTGTGAGGTTTTGCTGCATTATGTGAGCGAATATTAAATTCGTGAATATTGTAGtttga                                                                                                                             >  2:164075/1‑146 (MQ=255)
                 aaaatCTAATTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCaaa                                                                                                                            <  1:711929/146‑1 (MQ=255)
                                cGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATCCTCGCAAAGCTATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGATCAAAGCAAGTACCATTcga                                                                                                             >  1:646828/1‑147 (MQ=255)
                                cGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTAAAAACAATTACCATTCaa                                                                                                             >  1:646879/1‑149 (MQ=255)
                                             aaGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAtttt                                                                                                >  1:40707/1‑149 (MQ=255)
                                                       ccATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGaaaaa                                                                                      >  1:106400/1‑149 (MQ=255)
                                                          tttttCTCAATGCTTCTAATATATTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGAGAATATTAACTATGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTc                                                                                   <  1:1263/149‑1 (MQ=255)
                                                               cgcaagtcTTCTAGCATACTCGCAAAGATGACTAAAGGGCATCGTATGAGGTTTTGCTGCATTCTCGGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCAGTCAATTAATGTTATTTTTAGTGAAAAATTCtttta                                                                              <  2:626949/142‑1 (MQ=255)
                                                                  aaTGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCGTTCTCTGAACGAATATGAACTCTGTGATTTTTATAGTTAGAACAAAGTAGCATTCAATTAATGTTCTGTCTAGTGAAATATGCtttttttt                                                                           >  2:311796/1‑149 (MQ=255)
                                                                   aTGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAAGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAAATTATAGTTCAAAGCAATTACTATTCAATTAATTTTATTTTTATTAAAAAATTCttttatttt                                                                          >  2:65285/1‑149 (MQ=255)
                                                                             tataCTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCggg                                                                >  1:407622/1‑149 (MQ=255)
                                                                                acccccAAATATAAATAGAGAGCAACATATTAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGAtt                                                             <  2:181619/144‑1 (MQ=255)
                                                                                 cTCGCAAAGATAACTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGAttt                                                            <  2:392540/149‑1 (MQ=255)
                                                                                    gCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTGTTATTAGTAAAAAATTCTTTTATTTTATCGTCCGGGATTTTTc                                                         >  1:503550/1‑149 (MQ=255)
                                                                                    gCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATGTTATCGTCCGGGATTTTTc                                                         >  1:699339/1‑149 (MQ=255)
                                                                                       aaGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCga                                                      >  1:528450/1‑149 (MQ=255)
                                                                                                  agGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCa                                           <  1:108499/149‑1 (MQ=255)
                                                                                                   gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGAATTGAATTTCAg                                          >  1:326838/1‑149 (MQ=255)
                                                                                                     gtaTCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTGTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTGATCGTCCGGGATTTTTCCGACTTGACTTTCAGtt                                        <  2:581546/147‑1 (MQ=255)
                                                                                                      cATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATGTTATCGTCCGGGATTTTTCCTACTTGACTTTCAGTTc                                       >  1:48499/1‑149 (MQ=255)
                                                                                                            aTGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCt                                 >  1:65250/1‑149 (MQ=255)
                                                                                                             tGAGGTTTTGCTGCATTCTCTGAGCGAATATTAAATCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCtt                                >  1:583239/1‑149 (MQ=255)
                                                                                                                 gTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACt                            <  1:440315/149‑1 (MQ=255)
                                                                                                                         gcATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTGACTGTcaaagc                    >  1:514153/1‑149 (MQ=255)
                                                                                                                           aTTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTcaaagcaa                  >  2:357862/1‑149 (MQ=255)
                                                                                                                            ttCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTTCGACTTGACTTTCTGTTCTGTGATTAACTGTcaaaattac                 >  2:438871/1‑144 (MQ=255)
                                                                                                                             tctcTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTcaaagcaaag                <  2:659604/149‑1 (MQ=255)
                                                                                                                                     cGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATAcc        <  1:118644/149‑1 (MQ=255)
                                                                                                                                      gAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACca       <  1:293010/149‑1 (MQ=255)
                                                                                                                                      gAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGCCAAAGCAAAGATAATACca       >  1:51441/1‑149 (MQ=255)
                                                                                                                                           ttAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACcactcac  >  1:661975/1‑149 (MQ=255)
                                                                                                                                           ttAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCAACTGGACTTTCAGTTTTGGGGTTAACTTTCAAAGCAAAGATAATACcactcac  >  2:38743/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
ACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCAC  >  NC_000913_3_pae_tpiA/1197356‑1197643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑