Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,978,503 Δ776 bp insB1insA insB1, insA

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1978503–1979270 1979278 9–776 35 [0] [0] 35 insB1–insA insB1,insA

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 19785020 (0.000)32 (0.730) 28/266 0.7 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913_3_pae_tpiA 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein

ATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAAT  >  NC_000913_3_pae_tpiA/1979135‑1979425
                                                                                                                                                |                                                                                                                                                  
atgggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATaa                                                                                                                                                <  2:291156‑M2/5‑1 (MQ=255)
    gtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagaacacacaaaacactcaattacttaacataaatgTATAagtaa                                                                                                                                            >  2:480206‑M2/141‑147 (MQ=255)
       tttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATAc                                                                                                                                         >  1:818195‑M2/138‑149 (MQ=255)
              ttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaccataaatgTATAAGTCATActtttgtg                                                                                                                                  >  2:381100‑M2/131‑148 (MQ=255)
              ttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgtt                                                                                                                                  >  2:611731‑M2/131‑149 (MQ=255)
                  ctaatatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                              <  1:630180‑M2/23‑1 (MQ=255)
                  ctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacacttcatgatgtaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                              <  2:346543‑M2/23‑1 (MQ=255)
                   taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacataaaacggaggggggctgcgattttcaataatgcgtgatgcagaccacacaaaaaaagaaattacttaacataaatgGATAAGTCATACATTTGTTGTggg                                                                                                                             >  1:558090‑M2/126‑149 (MQ=255)
                       atttttcttaaattgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgctgcagatcacacaaaacactcaataacttaacataaatgTATAAGCTACACTCTTGTTTTGTGTGTa                                                                                                                         >  2:547656‑M2/122‑149 (MQ=255)
                           ttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTc                                                                                                                     >  1:412069‑M2/118‑149 (MQ=255)
                                  atcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGt                                                                                                              <  2:730439‑M2/39‑1 (MQ=255)
                                   tcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgctgatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGtt                                                                                                             <  1:795659‑M2/40‑1 (MQ=255)
                                        gcaactgtactcgtcactacccgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTaaaaa                                                                                                        <  1:790717‑M2/45‑1 (MQ=255)
                                             tgtactcgtcactacacgcacatacaaaggtgtagacctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGtttt                                                                                                   >  1:608416‑M2/100‑149 (MQ=255)
                                               tactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTc                                                                                                 >  2:534275‑M2/98‑149 (MQ=255)
                                               tactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTc                                                                                                 <  2:279716‑M2/52‑1 (MQ=255)
                                                 ctcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTAAAAAAGTTTTTcgc                                                                                               >  1:695988‑M2/96‑149 (MQ=255)
                                                                   tacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaaa                                                                             <  1:131503‑M2/72‑1 (MQ=255)
                                                                           aggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAg                                                                     <  1:534275‑M2/80‑1 (MQ=255)
                                                                                   tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATc                                                             <  2:120602‑M2/88‑1 (MQ=255)
                                                                                          ttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATgg                                                      <  1:611731‑M2/95‑1 (MQ=255)
                                                                                             aataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGAt                                                   <  1:687701‑M2/98‑1 (MQ=255)
                                                                                             aaaaatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGATACGCTAGCAAGCAAAAATGAAACAGAAGTAATCGAAATGGGAt                                                   >  1:241835‑M2/52‑149 (MQ=255)
                                                                                                 atgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTg                                               <  1:4434‑M2/102‑1 (MQ=255)
                                                                                                  tgcgttatgcagataacacaaaactataaattccttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCCGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAGTTAGatgtatc                                              <  2:347131‑M2/103‑4 (MQ=255)
                                                                                                     gtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAACCAAAAATCAAACAGGAATAATCGAAATAGAATGTTGcaca                                           >  2:453410‑M2/44‑149 (MQ=255)
                                                                                                       gatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGcaca                                         <  1:509680‑M2/108‑1 (MQ=255)
                                                                                                           cagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCa                                     >  1:32471‑M2/38‑149 (MQ=255)
                                                                                                              atcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCaaaa                                  <  2:759058‑M2/115‑1 (MQ=255)
                                                                                                               tcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTTCCCACAGtcaaatt                                 >  2:389087‑M2/34‑147 (MQ=255)
                                                                                                                            tcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTaaa                    <  2:695988‑M2/129‑1 (MQ=255)
                                                                                                                                   cttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGGTGTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAatcat             >  1:27876‑M2/14‑149 (MQ=255)
                                                                                                                                         ataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCta       >  2:87021‑M2/8‑149 (MQ=255)
                                                                                                                                            aatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAACTTAATAGCGACGTTGGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCCTCTGCTataa    >  1:568860‑M2/5‑149 (MQ=255)
                                                                                                                                              tgTATAAGTCATACTTTTGTTTTGGATGTATTTAAATCTGTTAAAAATTTTTTCGCTACGCTAACAAGCAAAAATGAAACAGAAATAATCTAAATGGGATGTTGCTCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTataaat  <  2:558090‑M2/147‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
ATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAAT  >  NC_000913_3_pae_tpiA/1979135‑1979425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.