New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	2412629 =	37 (0.940)	5 (0.140)	5/260	4.2	12.2%	intergenic (‑35/+48)	yfbU/yfbV	UPF0304 family protein/UPF0208 family inner membrane protein
	?	NC_000913_3_pae_tpiA	2412656 =	38 (1.040)					intergenic (‑62/+21)	yfbU/yfbV	UPF0304 family protein/UPF0208 family inner membrane protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAA  >  NC_000913_3_pae_tpiA/2412487‑2412775                                                                                                                                               |                                                                                                                                                   aataatTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAaagaa                                                                                                                                              >  1:673456/1‑149 (MQ=255) aataagCGTTTTCAGGCGACCGTAACCTTAAGCATTTGCCGGATAGAGCATAGTCATGATATTGTACTGATAAACCACAACAAGACGTTGCGAGTCGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAaagaa                                                                                                                                              <  2:686386/149‑1 (MQ=255)             cagtagACGATAACGTTCATGATTTGCCGGTACAAGCATAGTCATCTTTTTGTATTGATTAGACAAAATAAGACGTTGCGCGTTGGTCTTTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGc                                                                                                                                  <  2:373725/144‑1 (MQ=255)                   aCGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGTTCAGTCTGCcttctt                                                                                                                            >  2:71266/1‑149 (MQ=255)                   aCGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGCATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCcttctt                                                                                                                            <  1:336972/149‑1 (MQ=255)                      gTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACGGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCCCTCTACTACGGTGAAAAAAAAGAAACCTGAGTATGACTTCTTTta                                                                                                                         >  1:1641/1‑149 (MQ=255)                                 gtagtGCCGGATCGATCATAGTTATCATCTTGTACTTATTAGACAAAATAAGACGACGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATc                                                                                                              <  1:127482/145‑1 (MQ=255)                                                gCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTATTCAGGGGTCAATTACaa                                                                                               >  1:244170/1‑149 (MQ=255)                                                      tcatcaTCTTGTACTGAATAAACAAAAAAAGGCGTTGCGCGATGAGCAGAACCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAatcatc                                                                                         <  2:323172/149‑1 (MQ=255)                                                                 tACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTGTTGTATATGCGCAACAAGGGGTCAATTACAAATCATCAAGGAAAGttt                                                                              >  1:617800/1‑149 (MQ=255)                                                                     gagtAGACAAAATAAGACGTTGCGGGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATc                                                                          <  2:261443/146‑1 (MQ=255)                                                                           aCAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCTgttg                                                                    >  2:504522/1‑149 (MQ=255)                                                                              aaaTAAGTCGTTTGGCGTTGGTGATTTTCGTTGTTGACTGCTGTAGCACTTTACTCCGGTGTAAAAATAGAAGGCTTCGTATGCCTTCTTTTATATGCGTGCTCAGTGGTTAATTACAGCTCCTCTAGGAAAGTTTTATCCAGTTGttt                                                                 <  2:509875/149‑1 (MQ=255)                                                                                aTAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGATTTATCCAATTTTTTGa                                                               >  1:179485/1‑149 (MQ=255)                                                                                 tAAGAGGTTGCGCGTTGGTTATTTCCATGGTTGACTTCTGTATCCATCAACTACGGTGAAAAAAAAGAAGGCTGAGTATGCATTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGaa                                                              <  2:25615/149‑1 (MQ=255)                                                                                  aaGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                             <  1:95046/149‑1 (MQ=255)                                                                                        ttGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGgcgcgc                                                       >  1:343519/1‑149 (MQ=255)                                                                                                   tCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc                                            >  1:435220/1‑149 (MQ=255)                                                                                                     aTTTCCATTGTTGACTCCTGTATCCCTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTAGATGCGTAATCAGGGGTCAATTATAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAg                                          <  1:644112/149‑1 (MQ=255)                                                                                                         ccATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTaa                                      <  2:385990/149‑1 (MQ=255)                                                                                                          cATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAAt                                     >  2:505244/1‑149 (MQ=255)                                                                                                               ttgACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTg                                >  2:686562/1‑149 (MQ=255)                                                                                                                    tCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAAt                           >  1:83312/1‑149 (MQ=255)                                                                                                                     ccTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCaa                                                                                       <  2:553173/88‑1 (MQ=255)                                                                                                                     ccTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCaa                                                                                       >  1:553173/1‑88 (MQ=255)                                                                                                                       tGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                             <  2:33805/112‑1 (MQ=255)                                                                                                                       tGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                             >  1:33805/1‑112 (MQ=255)                                                                                                                           acACGCTCCTACGATGAAAAAAAAGAAGGCTAAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTTTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCtt                    <  1:78571/148‑1 (MQ=255)                                                                                                                             aCTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGGCTGCTAATCAGGCTTGc                  >  1:679628/1‑149 (MQ=255)                                                                                                                             aCTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGACTGCTAATCAGGCTTGc                  >  1:679787/1‑149 (MQ=255)                                                                                                                                 tactacGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTc              <  1:570048/149‑1 (MQ=255)                                                                                                                                    tacGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAAc           >  2:31293/1‑149 (MQ=255)                                                                                                                                     aaggTGAAAAAAAAGAAGGCTGAGGATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACg          <  2:682776/147‑1 (MQ=255)                                                                                                                                       ggTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAg                                                                                                            <  1:575773/49‑1 (MQ=255)                                                                                                                                       ggTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAg                                                                                                            >  2:575773/1‑49 (MQ=255)                                                                                                                                             aaaaaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCaa  >  1:606525/1‑149 (MQ=255)                                                                                                                                             aaaaaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCaa  <  2:83312/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                   AATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAA  >  NC_000913_3_pae_tpiA/2412487‑2412775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.