Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	275,065	G→A	T18I (ACC→ATC)	insH1 ←	IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	275,065	0	G	A	100.0%	34.4 / NA	14	T18I (ACC→ATC)	insH1	IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/5);  total (9/5)

TTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAA  >  NC_000913_3_pae_tpiA/275019‑275210                                               |                                                                                                                                                  ttttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGAATCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGGCTTGTTCGCACATTCCGGAGGCGTTATGAGCTgg                                             >  2:178668/1‑149 (MQ=255)   ttCTGGTCTGCCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTggcg                                           <  1:17938/149‑1 (MQ=255)        gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCt                                      >  2:461905/1‑149 (MQ=255)          cTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCttt                                    >  2:406793/1‑149 (MQ=255)           tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAg                                                           >  1:126675/1‑125 (MQ=18)           tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAg                                                           <  2:126675/125‑1 (MQ=18)              cgtcgcTCACCGCTGAATTCCCTGGCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGAGGACAAACATGATCTCATATCATGGACTTGTCCGTACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGt                                <  1:250382/146‑1 (MQ=17)              cGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGt                                <  1:492387/149‑1 (MQ=255)                          ctgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGAGCTATTGCTCCAGATGACAAACATGTTCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTCTTTGTCTCTGAttatta                    >  2:467753/1‑149 (MQ=18)                               ttCACTGTCGGCGAAGATAAATAAATAGCGTACTACGTACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTAGTTATTCATctc               >  2:503300/1‑147 (MQ=18)                                    tGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCAccc          >  2:224538/1‑149 (MQ=255)                                    tGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCAccc          >  2:455385/1‑149 (MQ=255)                                         gCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATTACAAACATGACCTCATATCAGGGACTTATTCGCACCTTCCGGAGGCGTTATTAGCTGGCGGCCCCTTTTGTATCTGATTATTCATCTCCACCCGCTAt     >  2:609753/1‑149 (MQ=21)                                            aaGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATGAATCCCCACCCGCTATTaa  <  2:589842/149‑1 (MQ=255)                                               |                                                                                                                                                  TTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAA  >  NC_000913_3_pae_tpiA/275019‑275210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑