Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 482,230 G→A P725L (CCG→CTG)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA482,2300GA98.0% 163.3 / NA 51P725L (CCG→CTG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (33/17);  minor base C (1/0);  total (34/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCC  >  NC_000913_3_pae_tpiA/482084‑482369
                                                                                                                                                  |                                                                                                                                           
tgacatAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCAGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCagg                                                                                                                                           <  1:233877/149‑1 (MQ=255)
    atAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTAt                                                                                                                                       >  2:627495/1‑149 (MQ=255)
        aCTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                   <  1:326644/149‑1 (MQ=255)
          tttCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCa                                                                                                                                 >  1:69026/1‑149 (MQ=255)
              ttcACACGACCGCGGTCGATAAAGTCGTTCACCTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGAcc                                                                                                                             >  2:295136/1‑149 (MQ=255)
                     gACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCCCATTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGa                                                                                                                      <  1:550679/149‑1 (MQ=255)
                      agcgcgGTGGATAAGGTCGTTCACATAGCTGGCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAGACACCCAGCGGCTGCGCTTTTTCCTGGTGAATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGAc                                                                                                                     <  2:640977/147‑1 (MQ=255)
                       ccGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTTGTCGATATCAATCTTTAACTGCACGCTATCTTCCAGACCGCTTGGACg                                                                                                                    >  2:332206/1‑149 (MQ=255)
                       ccGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACg                                                                                                                    >  2:650215/1‑149 (MQ=255)
                        cgcgGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGt                                                                                                                   <  1:539266/149‑1 (MQ=255)
                               gATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTgg                                                                                                            >  2:61660/1‑149 (MQ=255)
                                     gTCGTTCACATAGCTGCCGCCCCATGCAGCGACCAGAGTGGTGATAATGACGTTGATAGAAAGACCCAGCGCCTGCGCTTTTTCGCGAAAGCAGAAGATCGTAACCTGCAGGGTATCTGCCAGACCATTTGGACGTACGAGGGacaaca                                                                                                      >  1:162267/1‑149 (MQ=255)
                                        gTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGAc                                                                                                                     <  1:187157/131‑1 (MQ=255)
                                        gTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGAc                                                                                                                     >  2:187157/1‑131 (MQ=255)
                                                     ccgccCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgc                                                                                      <  1:209471/149‑1 (MQ=255)
                                                           cATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTa                                                                                                                  <  2:213660/115‑1 (MQ=255)
                                                           cATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTa                                                                                                                  >  1:213660/1‑115 (MQ=255)
                                                                   gcCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTACTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaagca                                                                        >  1:460668/1‑149 (MQ=255)
                                                                        gagTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTgg                                                                   <  1:61660/149‑1 (MQ=255)
                                                                               gtTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTAcgcg                                                            >  2:492127/1‑149 (MQ=255)
                                                                                        tttGATAGACACACCCCGCGACTGGCCTCTTTCCGTGTCGATATCAATCTTAAACTGCAGGTTATCTTCCAGACCGTTTCGACGTCCGCTGGTCAACATATCAGGGTGCTTAGCTGCTTCTGCAAGCAACTGATAACGCGCCTGATTcc                                                   <  2:448248/148‑2 (MQ=255)
                                                                                          tGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTTGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGt                                                 <  2:85352/149‑1 (MQ=255)
                                                                                           gATAGAAACACTCATCGCCTGCGCTTTTTGCTGGTCGATATCAATGTTACACTGCAGGGTATGTTCCAGACCGTTTGGACGTGCGATGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCCGtt                                                >  2:632114/1‑149 (MQ=255)
                                                                                            aTAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCCGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTCCTTCTGCAAGCCACTGGTTACCCACCTGCGTcccttt                                               >  2:568024/1‑144 (MQ=255)
                                                                                              aGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttttt                                             >  1:257416/1‑149 (MQ=255)
                                                                                              aGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttttt                                             >  2:248829/1‑149 (MQ=255)
                                                                                               gAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTGTTTc                                            >  1:592538/1‑149 (MQ=255)
                                                                                                 aaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                          <  2:479981/149‑1 (MQ=255)
                                                                                                    acCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGCCGTACGCTGGTCAACATATCAGGGTGCTTCACTGCTTCTGCAAGCAACTCGTTACGCGCCTGAGTCAGCTTTTCGTCAc                                       >  2:426270/1‑149 (MQ=255)
                                                                                                    acCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGCGTCAGTTTTTCGTGAc                                       >  2:426265/1‑149 (MQ=255)
                                                                                                    acCCAGCGCCTGCGCTTTTTCATGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTGGGACGTACGCTGTTCAACATATCAGGGTGCTTCGCTGCTTCTGCAACCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAc                                       >  2:553818/1‑149 (MQ=255)
                                                                                                       caccgcCTGCGCCTTCGCCTCTTCGATATCAATTTTAATCTGCAGGGTATCTTCCAGACCGTTTGGACCTACGCTGGTCAACATATCAGGGTGCTTCGCTGATTCTGCAAGCAACTGGTTACGAGCCTGAGTCAGTTTTTCGTGACCaa                                    <  2:312749/146‑1 (MQ=255)
                                                                                                         gcgcCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAgg                                  <  1:650215/149‑1 (MQ=255)
                                                                                                              tGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTAAGTTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTGTTCGTGACCAAGGCCAGc                             >  1:373185/1‑149 (MQ=255)
                                                                                                                  cTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTgg                         <  2:257416/149‑1 (MQ=255)
                                                                                                                   tttttCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGt                        >  2:444290/1‑149 (MQ=255)
                                                                                                                   tttttCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGAAAGCAACTGGTTACGCGACTGAGTCCATTTTTCGTGACCCCGGCCAGcccaac                        >  2:246565/1‑145 (MQ=255)
                                                                                                                     tttCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCa                      >  1:300728/1‑149 (MQ=255)
                                                                                                                        ccTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTAGTTTGTTCAACAAAACAGCGCGAGTGGCTGCTCCTGCAAGCAAGTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATc                   <  2:528260/149‑1 (MQ=255)
                                                                                                                          tGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCCGGGTGCTTCGCTTCTTCTGCAAGCAACTGGTTACACGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg                 >  2:343194/1‑149 (MQ=255)
                                                                                                                          tGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg                 >  1:666312/1‑149 (MQ=255)
                                                                                                                           ggTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCGGCCTGGTCAATCAGc                >  1:566035/1‑149 (MQ=255)
                                                                                                                           ggTCGATATCAATCCTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGc                <  1:441821/149‑1 (MQ=255)
                                                                                                                            gTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGCGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt               >  2:247679/1‑149 (MQ=255)
                                                                                                                            gTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAGCCAGGCCAGCCTGGTCACTTAGCt               >  1:331150/1‑149 (MQ=255)
                                                                                                                                  atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc                                 <  2:581188/125‑1 (MQ=255)
                                                                                                                                  atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc                                 >  1:581188/1‑125 (MQ=255)
                                                                                                                                  atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagt         >  1:320225/1‑149 (MQ=255)
                                                                                                                                  atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagt         >  1:20336/1‑149 (MQ=255)
                                                                                                                                   tCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACCTATCAGGGTGCTTCGCTGCTTCTGCAAGCACCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtc        >  1:255527/1‑149 (MQ=255)
                                                                                                                                       tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag    >  1:352056/1‑149 (MQ=255)
                                                                                                                                         ttAAACTGCAGGGTATCTTCCAGACCGTTTGCAATTACTCAGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTCACCAAGGCCCGCCTAGTCAATCAGCtcaaactcacaacc  >  2:328350/1‑144 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
TGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCC  >  NC_000913_3_pae_tpiA/482084‑482369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑