Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 257908 258683 776 48 [0] [0] 48 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 2579070 (0.000)40 (0.760) 35/268 0.6 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913_3_pae_tpiA 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

CGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAAT  >  NC_000913_3_pae_tpiA/257760‑258054
                                                                                                                                                   |                                                                                                                                                   
cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAa                                                                                                                                                    <  2:69622‑M1/149‑2 (MQ=255)
cGCTTGTGTTAAAAATTTGCTAAACTTTGCCAATTTGGTAAAACAGTTGAATCTAGTTTGAATAGATCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAa                                                                                                                                                    <  1:965316‑M1/149‑2 (MQ=255)
   ttGTGTTAAAAATGTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtg                                                                                                                                                 <  1:4963‑M1/149‑5 (MQ=255)
   ttGTGGTACAAATTTGCGACATTTTGCCAATTTGGTAAAACGGTTGCCTCACAACAGGAGATAGCAATGACGTTACCGAGCGGACACCCGAAGAGCAGATGGATCAAAAAATTCACCGCACTAGGCCCGTATATTCGTGAAGGTAagtg                                                                                                                                                 <  1:604655‑M1/149‑5 (MQ=255)
      tgtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaa                                                                                                                                              >  1:802776‑M1/1‑142 (MQ=255)
         tAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATGGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaaga                                                                                                                                           <  1:28270‑M1/149‑11 (MQ=255)
              aTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatc                                                                                                                                      <  1:74924‑M1/149‑16 (MQ=255)
                    tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattct                                                                                                                                >  1:298003‑M1/1‑128 (MQ=255)
                                 tttGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtct                                                                                                                   >  1:935166‑M1/1‑115 (MQ=255)
                                 tttGGTAAAACAGTTGCATAACAACAGGAAATAGCAATGACGTTACCGTGTGGACACCCGAAGAGCAGATTGACCAAAAAATTGACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaatgataatcgattctttttcgattgtct                                                                                                                   <  2:476597‑M1/149‑35 (MQ=255)
                                   tGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg                                                                                                                 <  2:649501‑M1/149‑37 (MQ=255)
                                       aaaaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgt                                                                                                             >  1:845867‑M1/1‑109 (MQ=255)
                                       aaaaCAGTTGCATAACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgt                                                                                                             <  2:882680‑M1/149‑41 (MQ=255)
                                         aaCAGTTGGATCACAACAGGAAATAGCAATGACGTTACCGAGGGGACACCCGAAGAGCATATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtat                                                                                                           <  2:387911‑M1/149‑43 (MQ=255)
                                         aaCAGTTGAATCACAACAGGAGATAGCCATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATGCGTGAAGGTAtgtgtaaagataatcgattctttttcgattgtctggctgtat                                                                                                           <  1:730562‑M1/149‑43 (MQ=255)
                                                  aTCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgatagtctggctgtatgcgtcaagg                                                                                                  >  2:649642‑M1/1‑98 (MQ=255)
                                                   tCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgt                                                                                                 <  2:546711‑M1/149‑53 (MQ=255)
                                                     acaacaGGAGATAGCAATGACATCACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtga                                                                                               <  1:748358‑M1/149‑55 (MQ=255)
                                                       aacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgcttctttttcgattgtctggcggtatgcgtcaacgtgaag                                                                                             >  1:101044‑M1/1‑93 (MQ=255)
                                                        acaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgatcgtctggctgtatgcgtcaacgtgaact                                                                                            >  2:820288‑M1/1‑92 (MQ=255)
                                                                  gCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccgga                                                                                  <  2:935166‑M1/149‑68 (MQ=255)
                                                                   gaaggacgtTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaa                                                                                 <  2:570558‑M1/145‑69 (MQ=255)
                                                                      tGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTAGTGAAGGTAagtgcaaagataatcgattctttttgcattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtg                                                                              >  1:304462‑M1/1‑78 (MQ=255)
                                                                              ccGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAattgtaaagataatcgattctttttcgattgtctggatgtatgcgtcaacgtgaaaaccgctccagaagtgcgtgaatt                                                                      >  2:223242‑M1/1‑70 (MQ=255)
                                                                                 aGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg                                                                   <  2:298003‑M1/149‑83 (MQ=255)
                                                                                 aGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATACTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg                                                                   <  1:151422‑M1/149‑83 (MQ=255)
                                                                                        acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgcctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctgg                                                            <  1:643586‑M1/149‑90 (MQ=255)
                                                                                         gccgTAGAGGAGATTGAGCAAAAAAGTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgactgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggt                                                           <  2:982878‑M1/148‑91 (MQ=255)
                                                                                               gagCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccgggagtgcgtgaattctggggctggtggatgg                                                     >  1:160915‑M1/1‑53 (MQ=255)
                                                                                                     atggatcAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgg                                               <  2:198661‑M1/146‑103 (MQ=255)
                                                                                                         aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgtttgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgaggctggtggatgcagcgtgaagc                                           >  2:809950‑M1/1‑43 (MQ=255)
                                                                                                          tCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcg                                          >  1:535970‑M1/1‑42 (MQ=255)
                                                                                                             aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcaattctttttcgattgtttgtctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggccggtgggttgagctggaagggcag                                       >  2:163539‑M1/1‑39 (MQ=255)
                                                                                                                    tACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatccc                                <  1:331419‑M1/149‑118 (MQ=255)
                                                                                                                                 ccGTATATTCGTGAAGGTAagtgcaaagataatcgcttctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgtgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacag                   >  1:381813‑M1/1‑19 (MQ=255)
                                                                                                                                 ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacag                   >  2:336808‑M1/1‑19 (MQ=255)
                                                                                                                                  cGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgatttacctacagt                  >  2:383751‑M1/1‑18 (MQ=255)
                                                                                                                                    tataTTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgga                                                        <  1:710216‑M1/109‑94 (MQ=255)
                                                                                                                                    tataTTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgga                                                        >  2:710216‑M1/1‑16 (MQ=255)
                                                                                                                                         tCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagt           <  1:241965‑M1/149‑139 (MQ=255)
                                                                                                                                            tGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttg        <  1:163539‑M1/149‑142 (MQ=255)
                                                                                                                                            tGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccgg                                                                                         <  1:80431‑M1/68‑61 (MQ=255)
                                                                                                                                            tGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccgg                                                                                         >  2:80431‑M1/1‑8 (MQ=255)
                                                                                                                                               aGGTAagtgcaaagatagtcgattctttttcgattgtctggctgtatgcgtcaacgtgcaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtc     <  2:364417‑M1/149‑145 (MQ=255)
                                                                                                                                                ggTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtct    <  1:336808‑M1/149‑146 (MQ=255)
                                                                                                                                                  tAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgt  >  2:181926‑M1/1‑2 (MQ=255)
                                                                                                                                                  tAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaa                                                                        >  2:645478‑M1/1‑2 (MQ=255)
                                                                                                                                                  tAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaa                                                                        <  1:645478‑M1/79‑78 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAAT  >  NC_000913_3_pae_tpiA/257760‑258054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.