Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA3,440,9820GA27.9% 114.2 / 54.5 79I16I (ATC→ATT) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (29/28);  new base A (9/13);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCT  >  NC_000913_3_pae_tpiA/3440835‑3441128
                                                                                                                                                   |                                                                                                                                                   
tagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGa                                                                                                                                                    <  1:247955/149‑1 (MQ=255)
 aggacacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGat                                                                                                                                                   <  2:571272/146‑1 (MQ=255)
  gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGata                                                                                                                                                  >  2:29457/1‑149 (MQ=255)
      acCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCaa                                                                                                                                              >  1:787317/1‑149 (MQ=255)
      acCATCAATCTCAACCTCGGTCACCACGCAACCCGGCATCGATCAGAGCAGAATACGGCGCAGTGCGTTGCCCAGAGTATGGTCAAAGCCACGCTCTAAAGCCTCAAGGATCACCTTGGCGTGCGTCGAATGCACTTGCTCAGTATCaa                                                                                                                                              >  1:750817/1‑149 (MQ=255)
       ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAc                                                                                                                                             <  2:232589/149‑1 (MQ=255)
       ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAAc                                                                                                                                             <  1:691578/149‑1 (MQ=255)
        cATCAGTCTCGACCTCGGTTACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAcc                                                                                                                                            <  1:117179/149‑1 (MQ=255)
        cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAcc                                                                                                                                            >  1:665319/1‑149 (MQ=255)
         aTCATTCTGAACGTCGGTCACCGCGCAGCGCGGCATCGATGAGAGGAGAATACGGCGCAGGGCGTTGCCCAGAGTATGGTCAAGGCCACGCGCGAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                                                                                           <  2:21706/149‑1 (MQ=255)
         aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCa                                                                                                                                           >  1:458136/1‑149 (MQ=255)
          tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAg                                                                                                                                          >  1:927686/1‑149 (MQ=255)
             gtctcAACCTCTGTCACCGCGCAACCCGGCATGGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATAGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTCGCTCGATACTAACGAGgcg                                                                                                                                       >  2:132503/2‑149 (MQ=255)
                 cAACCTCGTTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCGCCGCCTCAAACGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGt                                                                                                                                  <  1:925259/149‑1 (MQ=255)
                  aaCCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGt                                                                                                                                  >  1:616459/1‑148 (MQ=255)
                  aaCCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGt                                                                                                                                  <  2:616459/148‑1 (MQ=255)
                      tCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGACCTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAg                                                                                                                             <  2:861955/149‑1 (MQ=255)
                       cGGTCACCGCGCTGCCCGGTATGGATGAGAGCACGATTCGGCGCAGTGCGTTACCCAGAGTATGGTGCAAGCCACGCTCTAAAGGCTGAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGa                                                                                                                            <  2:787358/149‑1 (MQ=255)
                       cGGTCACCGCGCAACCCGGCAGCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGa                                                                                                                            <  2:787317/149‑1 (MQ=255)
                        ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGaa                                                                                                                           >  2:9454/1‑149 (MQ=255)
                         gTCACCGGGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGaaa                                                                                                                          <  2:8106/149‑1 (MQ=255)
                         gTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGaaa                                                                                                                          <  1:9454/149‑1 (MQ=255)
                               gcgcAACCCGGCATCTATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCGCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCAGTTGCTCGATATCAACCAGACG‑CGGTTTTAGAAACTCTGt                                                                                                                    >  2:809403/1‑149 (MQ=255)
                                agcAGCCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCAGCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACCCTGTc                                                                                                                   >  2:748705/2‑149 (MQ=255)
                                 gcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTAACTTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTca                                                                                                                  <  1:280736/149‑1 (MQ=255)
                                          cATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCt                                                                                                         <  1:534413/149‑1 (MQ=255)
                                          cATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGACCTCACGC‑GGCGTTTTTTTTAGAAACTCTGTCACAGAACCCt                                                                                                         <  1:974666/149‑1 (MQ=255)
                                               aTGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCAtt                                                                                                    <  1:29457/149‑1 (MQ=255)
                                                 gagaGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATtgt                                                                                                  <  2:783248/149‑1 (MQ=255)
                                                    agCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                                                                                           <  2:625670/106‑1 (MQ=255)
                                                    agCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                                                                                           >  1:625670/1‑106 (MQ=255)
                                                    agCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                                                                                               <  1:651205/149‑1 (MQ=255)
                                                     gCAGAACACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCGCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTcc                                                                                              <  1:91263/149‑1 (MQ=255)
                                                        gAATACGGCGCAGTGCGTCACCCAGAGTATGGTCAAAGCGACGCTCTCAAGGCTCAAGGGTCACCTTGGCGTGCGTCGACCTCACTTGCTCGATATCAACCAGGCA‑CGGTTTTAGAAACTCTGACACAGAACCCTGCATTGTGTCctct                                                                                           <  1:748705/149‑1 (MQ=255)
                                                         aaTACGGCGCAGTGCGCTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctctc                                                                                          <  1:922667/149‑1 (MQ=255)
                                                           tACGGCGCAGTGCGTTACCCAGAGGATGGTCAAAGCCACGCTCTAAAGGCGCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                                                                        <  2:589786/149‑1 (MQ=255)
                                                              ggCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACTTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTGCTCTCTTTgg                                                                                     <  2:856907/149‑1 (MQ=255)
                                                                ccaaGTGCATAACCCAGAGTACGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTa                                                                                   <  2:738271/146‑1 (MQ=255)
                                                                       cGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGGGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCt                                                                            <  1:185401/149‑1 (MQ=255)
                                                                           aCCCAGAGTTTGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGTCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTAc                                                                        <  1:519784/149‑1 (MQ=255)
                                                                             ccAGAGTATGGTCAAAGCCACGCTCTAAAGGCTGAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtt                                                                      >  1:829604/1‑149 (MQ=255)
                                                                             ccAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtt                                                                      >  2:724028/1‑149 (MQ=255)
                                                                             ccAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtt                                                                      <  1:81043/149‑1 (MQ=255)
                                                                              cAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTg                                                                     >  2:972353/1‑149 (MQ=255)
                                                                              cAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CTGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTg                                                                     <  1:925680/149‑1 (MQ=255)
                                                                                   tATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTa                                                                >  1:868000/1‑149 (MQ=255)
                                                                                      ggtcAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTGAAg                                                             >  1:95588/4‑149 (MQ=255)
                                                                                      ggtcAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAg                                                             >  1:790304/4‑149 (MQ=255)
                                                                                       gtcAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTaaa                                                              <  2:735946/145‑1 (MQ=255)
                                                                                       gtcAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTaaa                                                              >  1:735946/3‑147 (MQ=255)
                                                                                          aaaGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCCCTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAACCTTTACTTGGAGTaacggtca                                                         >  2:364206/1‑143 (MQ=255)
                                                                                          aaaGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                                                                                               >  1:950255/1‑111 (MQ=255)
                                                                                          aaaGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                                                                                               <  2:950255/111‑1 (MQ=255)
                                                                                           aaGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTcga                                                        <  1:614578/149‑1 (MQ=255)
                                                                                                aCGCTCTAGAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGATAGTCTGTCACAGAACACTGCATTGTGTCCTCTCTTTGGTACTAAGCTGTGCTTGAAGTAAAGCTCGACGATc                                                   >  2:833469/1‑149 (MQ=255)
                                                                                                aCGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGATGATc                                                   <  1:972353/149‑1 (MQ=255)
                                                                                                  gCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAg                                                 >  2:741154/1‑149 (MQ=255)
                                                                                                    gctAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTCCTTGTCGTAAAGCTCGACGAtagggt                                               >  2:754065/2‑144 (MQ=255)
                                                                                                      tAAAGGCTCAAGGGTCACCTTGGCTTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCttt                                                                                       >  1:581450/1‑107 (MQ=255)
                                                                                                      tAAAGGCTCAAGGGTCACCTTGGCTTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCttt                                                                                       <  2:581450/107‑1 (MQ=255)
                                                                                                      tAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGgtgt                                             >  1:983689/1‑149 (MQ=255)
                                                                                                      tAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGgtgt                                             >  1:363944/1‑149 (MQ=255)
                                                                                                        aaGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGGACTAAGCTTTACTTGGAGTAAAGCTCGACGCTCAGGTGTTc                                           >  2:55340/1‑149 (MQ=255)
                                                                                                         aGGCTCAAGGGTCACCTTGGCGTGCGGCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCg                                          <  1:51561/149‑1 (MQ=255)
                                                                                                                        cTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCGCTTTGGTACTAAGCTTTACTTGGAGGAAAGCTCGACGATCCTGTGTTCGTTAGTCTCCGcagac                           >  2:130296/1‑149 (MQ=255)
                                                                                                                        cTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGAt                                                    <  1:15785/124‑1 (MQ=255)
                                                                                                                        cTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGAt                                                    >  2:15785/1‑124 (MQ=255)
                                                                                                                              gtgcgtCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCa                     >  1:729158/1‑149 (MQ=255)
                                                                                                                                 cgtCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGaa                  <  1:414136/149‑1 (MQ=255)
                                                                                                                                 agtCGAACTTCCTTGCTAAATATCAACTAGGCC‑CGGTTTTTGAAACTCTGTCACAGAAGCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACCTGGAGTAAAGCGTTACGATCAGGTGTGCGTTAATGTCCGCAGTGATATCAGaa                  <  2:18174/148‑1 (MQ=255)
                                                                                                                                   tCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGGGTTCGTTAATGGCCGCAGACAGATCAGAACg                >  1:216757/1‑149 (MQ=255)
                                                                                                                                        cTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCg           >  1:426386/1‑149 (MQ=255)
                                                                                                                                         tCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCgg          >  1:593704/1‑149 (MQ=255)
                                                                                                                                          cACGTGGTCGATATTAACCAGGCC‑GGGTTTTAGCAACGCTGTCACAGAACCCGGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAGGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGc         <  1:407496/149‑1 (MQ=255)
                                                                                                                                             ttGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGAt                       >  1:926881/1‑132 (MQ=255)
                                                                                                                                             ttGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGAt                       <  2:926881/132‑1 (MQ=255)
                                                                                                                                             ttGCTCGATATCAACCAGGCG‑CGGTCTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGAAGTAAAGCTCGACGCTCAGGTGTTCGTTAATATCCGTAGACAGATCAAAACGCTCCGGCTTa      >  2:764285/1‑149 (MQ=255)
                                                                                                                                               gCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGATTTACTTGTAGTAAAGACCTTGGATGGGGGGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACg    >  1:187165/1‑149 (MQ=255)
                                                                                                                                                 tCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGCTCAGAACGCTCCGGCTTACGCt  >  1:697376/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCT  >  NC_000913_3_pae_tpiA/3440835‑3441128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑