Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	3,440,923	C→T	G36D (GGC→GAC)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	3,440,923	0	C	T	100.0%	251.1 / NA	81	G36D (GGC→GAC)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/47);  total (34/47)

GTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTA  >  NC_000913_3_pae_tpiA/3440778‑3441066                                                                                                                                                   |                                                                                                                                                 gTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCaa                                                                                                                                               >  1:202893/1‑149 (MQ=255)      gCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCa                                                                                                                                          >  1:783248/1‑149 (MQ=255)       cAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCAc                                                                                                                                         >  1:318032/1‑149 (MQ=255)       cAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGGATGGTCAAAGCCAc                                                                                                                                         >  1:166898/1‑149 (MQ=255)              tCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGACGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTaa                                                                                                                                  >  1:590327/1‑149 (MQ=255)               ccAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATGAATCTCAACCTCGGCCACCGCGCAACCCGGCATCCATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTaat                                                                                                                                 <  2:745812/149‑2 (MQ=255)               ccAGCATATCTTCCCGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTaaa                                                                                                                                 <  2:73258/149‑1 (MQ=255)                  ggATATTGTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGc                                                                                                                              <  2:509299/149‑1 (MQ=255)                  ggATATCTTCCTGAAGGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCCCGGCGCAAACCGGCAACGATGAGAGCAGAATACGGCGCA‑GAGCGTTACCCAGATTATGGTCAAACCATCGCTCCAATggg                                                                                                                              >  2:46607/1‑148 (MQ=255)                     tatCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCa                                                                                                                           <  2:643904/149‑1 (MQ=255)                       tCTTCCTGAACGCCTTCTTGGGTGCTGTACTCATGTAGTACACCATCAATCTCACCCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAg                                                                                                                         <  2:660540/149‑1 (MQ=255)                        cTTCCTTAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                        <  1:641533/149‑1 (MQ=255)                           ccTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTc                                                                                                                     <  2:556631/149‑1 (MQ=255)                           ccTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTc                                                                                                                     <  1:827442/149‑1 (MQ=255)                                 cGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCCACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTg                                                                                                               <  1:173059/149‑1 (MQ=255)                                     ttcttTGGTGCTGTACTCATGTAGCACACCATCAATCTCAACCTCGGTCACCGCGTCCGATATCGTTGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgt                                                                                                           <  1:723173/149‑1 (MQ=255)                                      tcttTGGTGCTGTACTCCTGTAGTACACCATCAATCTCCCCCTCGGTCACCGCGCATCCCGGCACCGATGTATGCTTAAT‑CGCATTATTTTCTTTTCCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtg                                                                                                          <  1:481131/149‑1 (MQ=255)                                             tGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGaa                                                                                                   <  1:817792/149‑1 (MQ=255)                                                tGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTc                                                                                                >  1:208313/1‑149 (MQ=255)                                                 gTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCa                                                                                               >  2:974666/1‑149 (MQ=255)                                                  tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGACATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTCCGTCGGACTCAc                                                                                              >  1:332419/1‑149 (MQ=255)                                                   aCTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGCGGACCTTACGGCGCC‑GTGAGCCACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGGGTCGAACTCACt                                                                                             >  1:232589/1‑149 (MQ=255)                                                        tGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATGCGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCTTGCGTCAAACTCACTTGCtg                                                                                        >  2:2592/1‑148 (MQ=255)                                                        tGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                        >  2:925680/1‑149 (MQ=255)                                                          tagtaCACCATCAATCTCAACCTCGGTCGCTGCTCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTcaa                                                                                      >  2:708759/1‑147 (MQ=255)                                                          tagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGa                                                                                      <  1:247955/149‑1 (MQ=255)                                                           aggacacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGat                                                                                     <  2:571272/146‑1 (MQ=255)                                                            gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGata                                                                                    >  2:29457/1‑149 (MQ=255)                                                                acCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCaa                                                                                >  1:787317/1‑149 (MQ=255)                                                                acCATCAATCTCAACCTCGGTCACCACGCAACCCGGCATCGATCAGAGCAGAATACGGCGCA‑GTGCGTTGCCCAGAGTATGGTCAAAGCCACGCTCTAAAGCCTCAAGGATCACCTTGGCGTGCGTCGAATGCACTTGCTCAGTATCaa                                                                                >  1:750817/1‑149 (MQ=255)                                                                 ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAc                                                                               <  2:232589/149‑1 (MQ=255)                                                                 ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAAc                                                                               <  1:691578/149‑1 (MQ=255)                                                                  cATCAGTCTCGACCTCGGTTACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAcc                                                                              <  1:117179/149‑1 (MQ=255)                                                                  cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAcc                                                                              >  1:665319/1‑149 (MQ=255)                                                                   aTCATTCTGAACGTCGGTCACCGCGCAGCGCGGCATCGATGAGAGGAGAATACGGCGCA‑GGGCGTTGCCCAGAGTATGGTCAAGGCCACGCGCGAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                             <  2:21706/149‑1 (MQ=255)                                                                   aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGctc                                                                                                                                     >  2:73149/1‑93 (MQ=255)                                                                   aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGctc                                                                                                                                     <  1:73149/93‑1 (MQ=255)                                                                   aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCa                                                                             >  1:458136/1‑149 (MQ=255)                                                                    tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAg                                                                            >  1:927686/1‑149 (MQ=255)                                                                       gtctcAACCTCTGTCACCGCGCAACCCGGCATGGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATAGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTCGCTCGATACTAACGAGgcg                                                                         >  2:132503/2‑149 (MQ=255)                                                                           cAACCTCGTTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCGCCGCCTCAAACGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGt                                                                    <  1:925259/149‑1 (MQ=255)                                                                            aaCCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGt                                                                    <  2:616459/148‑1 (MQ=255)                                                                            aaCCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGt                                                                    >  1:616459/1‑148 (MQ=255)                                                                              ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                        >  1:736854/1‑95 (MQ=255)                                                                              ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                        <  2:736854/95‑1 (MQ=255)                                                                               cTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                        <  2:39665/94‑1 (MQ=255)                                                                               cTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                        >  1:39665/1‑94 (MQ=255)                                                                                tCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGACCTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAg                                                               <  2:861955/149‑1 (MQ=255)                                                                                 cGGTCACCGCGCTGCCCGGTATGGATGAGAGCACGATTCGGCGCA‑GTGCGTTACCCAGAGTATGGTGCAAGCCACGCTCTAAAGGCTGAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGa                                                              <  2:787358/149‑1 (MQ=255)                                                                                 cGGTCACCGCGCAACCCGGCAGCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGa                                                              <  2:787317/149‑1 (MQ=255)                                                                                  ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGaa                                                             >  2:9454/1‑149 (MQ=255)                                                                                   gTCACCGGGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGaaa                                                            <  2:8106/149‑1 (MQ=255)                                                                                   gTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGaaa                                                            <  1:9454/149‑1 (MQ=255)                                                                                      aCCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTc                                                                                                >  2:898004/1‑111 (MQ=255)                                                                                      aCCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTc                                                                                                <  1:898004/111‑1 (MQ=255)                                                                                         gcgcAACCCGGCATCTATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCGCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCAGTTGCTCGATATCAACCAGACG‑CGGTTTTAGAAACTCTGt                                                      >  2:809403/1‑149 (MQ=255)                                                                                          agcAGCCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCAGCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACCCTGTc                                                     >  2:748705/2‑149 (MQ=255)                                                                                           gcAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTAACTTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTca                                                    <  1:280736/149‑1 (MQ=255)                                                                                                    cATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCt                                           <  1:534413/149‑1 (MQ=255)                                                                                                    cATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGACCTCACGC‑GGCGTTTTTTTTAGAAACTCTGTCACAGAACCCt                                           <  1:974666/149‑1 (MQ=255)                                                                                                         aTGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCaaa                                                                                                                                              >  1:396603/1‑46 (MQ=255)                                                                                                         aTGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCaaa                                                                                                                                              <  2:396603/46‑1 (MQ=255)                                                                                                         aTGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCAtt                                      <  1:29457/149‑1 (MQ=255)                                                                                                           gagaGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATtgt                                    <  2:783248/149‑1 (MQ=255)                                                                                                              agCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                             >  1:625670/1‑106 (MQ=255)                                                                                                              agCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                             <  2:625670/106‑1 (MQ=255)                                                                                                              agCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                                 <  1:651205/149‑1 (MQ=255)                                                                                                               gCAGAACACGGCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCGCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTcc                                <  1:91263/149‑1 (MQ=255)                                                                                                                  gAATACGGCGCA‑GTGCGTCACCCAGAGTATGGTCAAAGCGACGCTCTCAAGGCTCAAGGGTCACCTTGGCGTGCGTCGACCTCACTTGCTCGATATCAACCAGGCA‑CGGTTTTAGAAACTCTGACACAGAACCCTGCATTGTGTCctct                             <  1:748705/149‑1 (MQ=255)                                                                                                                   aaTACGGCGCA‑GTGCGCTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctctc                            <  1:922667/149‑1 (MQ=255)                                                                                                                     tACGGCGCA‑GTGCGTTACCCAGAGGATGGTCAAAGCCACGCTCTAAAGGCGCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                          <  2:589786/149‑1 (MQ=255)                                                                                                                        ggCGCA‑GTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACTTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTGCTCTCTTTgg                       <  2:856907/149‑1 (MQ=255)                                                                                                                          ccaa‑GTGCATAACCCAGAGTACGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTa                     <  2:738271/146‑1 (MQ=255)                                                                                                                                  cGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGGGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCt              <  1:185401/149‑1 (MQ=255)                                                                                                                                      aCCCAGAGTTTGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGTCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTAc          <  1:519784/149‑1 (MQ=255)                                                                                                                                        ccAGAGTATGGTCAAAGCCACGCTCTAAAGGCTGAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtt        >  1:829604/1‑149 (MQ=255)                                                                                                                                        ccAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtt        >  2:724028/1‑149 (MQ=255)                                                                                                                                        ccAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtt        <  1:81043/149‑1 (MQ=255)                                                                                                                                         cAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTg       >  2:972353/1‑149 (MQ=255)                                                                                                                                         cAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCAATATCAACCAGGCG‑CTGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTg       <  1:925680/149‑1 (MQ=255)                                                                                                                                              tATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTa  >  1:868000/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                 GTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCA‑GTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCG‑CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTA  >  NC_000913_3_pae_tpiA/3440778‑3441066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑