Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 3,598,744 G→T A305A (GCC→GCA)  livJ ← branched‑chain amino acid ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA3,598,7440GT97.5% 238.2 / NA 81A305A (GCC→GCA) livJbranched‑chain amino acid ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (40/38);  total (40/41)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAG  >  NC_000913_3_pae_tpiA/3598601‑3598889
                                                                                                                                               |                                                                                                                                                 
ccGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTa                                                                                                                                              >  1:294402/1‑149 (MQ=255)
  gAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCAAGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCATGCAAAAATTGCAGCGCTGCGTaag                                                                                                                                            >  1:968677/1‑147 (MQ=255)
     cTCAAAGCCGTGCAGATCGCCTTTCGCATCCGAGGTCAGCGGTCCCATTGCGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCTGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAggtgg                                                                                                                                         <  2:443409/149‑1 (MQ=255)
        aaaGCCTTTCAGATCGCCTTTCTCATCCCAGGGCAGCGGTCCCATTACGGTATCCACGGAGCGCCCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTcc                                                                                                                                      >  2:744306/1‑149 (MQ=255)
           gCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCaaa                                                                                                                                   <  1:304139/149‑1 (MQ=255)
           gCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCaaa                                                                                                                                   <  2:866117/149‑1 (MQ=255)
           gCCTGTCGGTTCGCCTTTCTCGTCCCAGGTCGGCGGTCCGATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCaaa                                                                                                                                   <  2:396999/149‑1 (MQ=255)
              tttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGa                                                                                                                                >  2:36710/1‑149 (MQ=255)
              tttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTACGCCGTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGCCTGATTTTCGCCCTTCTGGTAAGATTTCAGCGCTGCGTGGGTTGGCCAAACGa                                                                                                                                >  1:85866/1‑149 (MQ=255)
                    aTCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGCCTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATgcgc                                                                                                                          >  1:669489/1‑149 (MQ=255)
                    aTCGCCTTTCTCATCACAGGTCAGCGGTCCCATTCCGGTAACCACGGAATTCGCCTTAAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATACAGGCCCGCGTTCAAAGATTGCATCAATGCGTAAAAGGTCCAAACAGATgcgc                                                                                                                          >  2:341425/1‑149 (MQ=255)
                        cctgccccATCCCGGGTTAGCGGTCCCATTACGGCATCCACCGTGCTCGCTTTCAGGCATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACt                                                                                                                      <  1:81365/142‑1 (MQ=255)
                        ccTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACt                                                                                                                      >  1:492732/1‑149 (MQ=255)
                          tttCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTTGCTATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTg                                                                                                                    >  2:411828/1‑149 (MQ=255)
                             ctcATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGt                                                                                                                 >  1:351974/1‑149 (MQ=255)
                                aTCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGCTCGTCAGACTGATTCAGGCCCGCCTGCAACGATTGCAGCGCTGCGTAGGTGGTCCACACGAATGCGCACCTTGGGTctt                                                                                                              >  2:244210/1‑147 (MQ=255)
                                aTCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGTATCGCTTCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCt                                                                                                              >  1:677541/1‑149 (MQ=255)
                                 tCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTg                                                                                                             >  1:94974/1‑149 (MQ=255)
                                     aGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGtttt                                                                                                         >  1:526655/1‑149 (MQ=255)
                                            cGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTAAGACTGATTCAGGCGCGCATGCAAAGATTCCAGCGCTGCGTAGGTGGTACAAACGAATCGCCCACTTGGCTCCTGttttttacgct                                                                                                  >  2:13713/1‑144 (MQ=255)
                                             ggccccATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCttt                                                                                                 <  2:965736/146‑1 (MQ=255)
                                             ggTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTc                                                                                                                                       <  1:443224/111‑1 (MQ=255)
                                             ggTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTc                                                                                                                                       >  2:443224/1‑111 (MQ=255)
                                                 ccATTACGGTATCCACGGAGTTCGCTTTCACGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCCGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATc                                                                                             <  2:821832/149‑1 (MQ=255)
                                                 ccATTACGGTATCCACGGAGTTCGCTGTCCGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATc                                                                                             <  1:796785/149‑1 (MQ=255)
                                                  cATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATcg                                                                                            >  1:113854/1‑149 (MQ=255)
                                                   aTTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATcgc                                                                                           <  1:214870/149‑1 (MQ=255)
                                                   aTTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATcgc                                                                                           <  2:113854/149‑1 (MQ=255)
                                                   aTTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATcgc                                                                                           <  1:214955/149‑1 (MQ=255)
                                                        ggTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTcaa                                                                                      <  1:7302/149‑1 (MQ=255)
                                                              cACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATgg                                                                                >  2:188018/1‑149 (MQ=255)
                                                               aCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCCAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACCTGGGTCCTGTTTTTTCGCTTTGCTCGCGTCAACAATggg                                                                               >  2:52387/1‑149 (MQ=255)
                                                               aCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCACCCATggg                                                                               >  2:155667/1‑149 (MQ=255)
                                                               aCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGCTTTTGCGTTTTGATCGCGTCAACAATggg                                                                               >  2:157309/1‑149 (MQ=255)
                                                                    gTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCACCAATGGGTTTGt                                                                          >  2:642181/1‑149 (MQ=255)
                                                                     ttCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGtt                                                                         >  1:388718/1‑149 (MQ=255)
                                                                      tCGCTTTGATGTATGTGGCGATTGCAGCCGTATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCTACCGCGTGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTc                                                                        <  1:191300/149‑1 (MQ=255)
                                                                        gCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGc                                                                      <  2:492732/149‑1 (MQ=255)
                                                                         ctgtCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGGCCGCATGCAAAGATTGCAGCGCTGCGTAGGTTGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTTATCGCGTCAACAATGGGTTTGTTCGcc                                                                     <  2:588672/146‑1 (MQ=255)
                                                                          tttCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCg                                                                    >  1:33721/1‑149 (MQ=255)
                                                                                gTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCCGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCt                                                              >  1:32325/1‑149 (MQ=255)
                                                                                 tATTTGGCGATTTCAGCCGGATGGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCTTGTTTTTTCGCTTTGATGGCGTCAACAATGGGTTTGTTCGCCGGAACCTg                                                             <  2:91578/149‑1 (MQ=255)
                                                                                  aTTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGa                                                            <  2:388718/149‑1 (MQ=255)
                                                                                  aTTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGa                                                            <  1:116993/149‑1 (MQ=255)
                                                                                  aTTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGa                                                            <  1:274029/149‑1 (MQ=255)
                                                                                   tttGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGAt                                                           <  1:287898/149‑1 (MQ=255)
                                                                                       gccaTTTCAGGCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTa                                                       <  2:937587/146‑1 (MQ=255)
                                                                                       gCGATTTCAGCCGGATCGTCAGACTGATTTAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTa                                                       <  1:845640/149‑1 (MQ=255)
                                                                                        cGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAg                                                      <  2:94974/149‑1 (MQ=255)
                                                                                        agATTTCAGCCGGATCGTCAGACGTATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAg                                                      <  2:294402/148‑1 (MQ=255)
                                                                                          acttCAACCGGATCGTCAGACAGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGtt                                                    <  2:145548/147‑1 (MQ=255)
                                                                                           tttCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTACTAGCGCTGTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGttg                                                   >  1:722339/1‑148 (MQ=255)
                                                                                           gttCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGttc                                                   <  2:824120/148‑1 (MQ=255)
                                                                                              cAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGttcttc                                                <  2:822011/149‑1 (MQ=255)
                                                                                              cAGCCGGATCGACAGACCGAGTCACGCCCGCCAGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATTGGTTTGTTCGCTGGAAACTGATCGTAGttcttc                                                <  2:49992/149‑1 (MQ=255)
                                                                                               aGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCg                                               <  2:932832/149‑1 (MQ=255)
                                                                                                 ccGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAGCAATGGGTTTGTTCGCCGGAACCTGATCCTCCTTCTTCGGc                                             >  2:622850/1‑149 (MQ=255)
                                                                                                 ccGGATCGGCAGACTCATTCCGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTGTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGc                                             <  1:390017/149‑1 (MQ=255)
                                                                                                   ggATCGTCAGACTTATTCAAGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCCCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGCTCGTAGGTCTTCGGCtt                                           >  2:485923/1‑149 (MQ=255)
                                                                                                   ggATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACATGAGTCCTGTTTTTTCGCTTTGATCGCGTAAACAATGGGTTTGTTCGCCCGAACCTGCTACGAGTTCTTCAGCtt                                           >  2:121054/1‑149 (MQ=255)
                                                                                                    gATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTg                                          <  1:940877/149‑1 (MQ=255)
                                                                                                         tccgACTGCGTCAGGCCCGCCTGCAAAGATTGCAGCGCTGTGTGGGTTGTCCAAACGAATGCGCCAGTTGGGCCCTGGTTTTTCGCTTTGATCGCGTCAACAAGGGGTTTGTTCGGCGGAACCTGATCGTAGTTCTTCGGCTTGGTCAc                                     <  2:52072/146‑1 (MQ=255)
                                                                                                         tCAGACTGATTCCGGCCCGACTGCAAAGATTGCACCGCTGCGTAGGTGGTCCAAACGAATGCGACACCTGGGTCCTGTTCTTTCGCTTTGATCCCGTAAAAAATGGATCTGTTCGCCGGCACCTAATCCTTCTTCTTCGCCTTGTTCAc                                     >  2:192591/1‑149 (MQ=255)
                                                                                                             aCTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGAGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACcagc                                 >  1:634454/1‑149 (MQ=255)
                                                                                                              cTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCAGCAAAGGAGTGCCAAACCGATTCGGCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACcagca                                <  2:351974/149‑1 (MQ=255)
                                                                                                               tGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACcagcag                               <  1:249869/149‑1 (MQ=255)
                                                                                                               tGATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACcagcag                               >  1:711335/1‑149 (MQ=255)
                                                                                                                gATTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGGAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCagcagc                              <  1:411828/149‑1 (MQ=255)
                                                                                                                 aTTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGcc                             >  2:925404/1‑149 (MQ=255)
                                                                                                                 aTTCAGGCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGGTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTGACCGGAAGcc                             >  2:730375/1‑149 (MQ=255)
                                                                                                                      ggCCCGCCTGCAAAGATGCTAGGGCTGCTTAGATGGTTCAAACGAATCGGCCTCTTGGGTCCTGTTGTTTCGCTGTGATCGCGTCAACAATGGGTTTGTTTGCTGGAACCTGATCGTAGTTCTGCGGCTTGGTCTCCAGCAGCCTTTcc                        <  2:511595/149‑1 (MQ=255)
                                                                                                                       gcccgcccGCAAAGATTGCAGCGCTGCGTCGGTGTTCCAAACGAATGCGCCAGTTGCGTCCAGAGCTTTCGCTTTGATCGCGTCAACCACGGGTTTGTTCGCCGGCACCTGATCGTAGTTCTTCGGCTTTGTCACCCGCAGACCTTCCg                       >  2:663351/1‑149 (MQ=255)
                                                                                                                       gCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCg                       >  1:262114/1‑149 (MQ=255)
                                                                                                                       gCCCGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCATCCg                       >  1:200583/1‑149 (MQ=255)
                                                                                                                          cGCCTGCAAAGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTg                    >  1:378368/1‑149 (MQ=255)
                                                                                                                            cctggcaaGATTGCAGCGCGGCGTAGGGGGTCCATACGGATGGGCCCCTTGGGTCCTGTTTTTTCGCTTTGATCGCGTTAACAATGGTTTTGTTCGCCGGATCCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGAt                  <  2:111253/143‑1 (MQ=255)
                                                                                                                                 aaaGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCCGCCATTCCGCTGATTcccc             >  2:520615/1‑149 (MQ=255)
                                                                                                                                 aaaGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGcc             >  2:130233/1‑149 (MQ=255)
                                                                                                                                  aaGATTGCAGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGGCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGccc            <  2:33721/149‑1 (MQ=255)
                                                                                                                                          aGCGCTGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTGGGTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGtt    <  2:526655/149‑1 (MQ=255)
                                                                                                                                           gcgcTGCGGGGGTGGTCCCAACGAATGCGCTACTTGGGTCCTGTTTTTTCTCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGGCCTTCCGCTGATTCGCCCGCAATGTTa   <  1:950081/149‑1 (MQ=255)
                                                                                                                                            cgctGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTTTTTTTTTTTTCATCAACAATCGGCTTGTTCGCCGCAACCGCATCTTAGTTCTTCGGCCTGGTCCCCTGCAGCCCTTCCCCTGATTCGCCCGCAATGtgac  >  2:234782/1‑146 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCTGATCGTAGTTCTTCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAG  >  NC_000913_3_pae_tpiA/3598601‑3598889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑