| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 275,065 | G→A | T18I (ACC→ATC) | insH1 ← | IS5 transposase and trans‑activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 275,065 | 0 | G | A | 96.0% | 57.6 / ‑5.5 | 25 | T18I (ACC→ATC) | insH1 | IS5 transposase and trans‑activator |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (13/11); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
CGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTAT > NC_000913_3_pae_tpiA/275002‑275207 | cGGGACAAGAAAATATCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACAGTCGGCGGAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGCTCTCATAACACGGACTTGTGACCACCTTCCg > 1:21318/1‑149 (MQ=11) ggACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGa < 1:633747/149‑1 (MQ=17) gACAAGAAAAGGTCCGTTCGGCGCTGACGGCGGTTACTTGTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAGCCCTGTTCTATGGTGCCAGATGACAAACAGGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAg < 2:879243/149‑1 (MQ=11) aaGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGGGGCg > 1:374743/1‑149 (MQ=17) aTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCGTATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGa > 1:920317/1‑149 (MQ=255) ctctTTTCTGGTCTGACGGCGCTCACTGCTGAATTAACTTGGGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGAGGTTATGacc > 2:168273/1‑147 (MQ=11) tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCtttt > 2:96142/1‑149 (MQ=255) ttACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGCTGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGa > 2:286532/1‑149 (MQ=255) ttACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATCAACCTTTTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTgattctta > 1:686988/1‑142 (MQ=17) tACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGat < 2:270275/149‑1 (MQ=255) aCTGCTGATTTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGatt < 2:766991/149‑1 (MQ=255) tgctgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAAGCCTGTTCTATGGCTCCAGAGTACAAACATGATCTCATGTCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCAGGCGCCCCTTTTTGTATCTGattat > 1:888396/1‑149 (MQ=18) tgctgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGattat > 1:520951/1‑149 (MQ=255) tgctgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGatt > 1:715199/1‑147 (MQ=255) tgctgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGatt < 2:715199/147‑1 (MQ=255) gctgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCAACAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCAGCTTGCGGAGGCGTTATGAGCTGGCGGCCCTGTTTGTATCTGgttatt > 1:85697/1‑149 (MQ=18) ttaaaTTCACTCTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATTACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCTGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGAttatta < 2:459198/146‑1 (MQ=18) ctgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATTATGAACCGTGTTGGACGGCTCCAGATAACAAACATTACATCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGAttatta < 2:743295/149‑1 (MQ=18) tgAATTCACTGGCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTaa < 2:582481/149‑1 (MQ=255) gAATTCGCTGTGGGACCAGATAAGTTGATGACTCATGATGACCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGTACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGTCGGCCCTTTTTGTATCTGATTATTAAt < 2:936760/149‑1 (MQ=18) gAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGCAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAAt > 2:842351/1‑149 (MQ=255) gAATGCTCTGTCGGCGAATATATGTTAGTGACCCATGATAAACCCGCTTCTATGGCCCCAGAGGACAAACATGATCTCATATCAGGGACTTTTTCGCACCTTCCTGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAAt < 2:937546/149‑1 (MQ=18) aagtCACTGTGGGCGAAGATAAGTTGATGACTCATGATGAAACCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGTACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTAACAATc < 2:561042/146‑1 (MQ=18) tGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCAccc > 1:415421/1‑149 (MQ=255) gCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTAt > 1:885798/1‑149 (MQ=255) | CGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTAT > NC_000913_3_pae_tpiA/275002‑275207 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |