Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 257908 258683 776 60 [0] [0] 60 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 2579070 (0.000)56 (0.920) 44/266 0.4 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913_3_pae_tpiA 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

GATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGT  >  NC_000913_3_pae_tpiA/258539‑258826
                                                                                                                                                 |                                                                                                                                              
ttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTg                                                                                                                                             <  2:231458‑M2/4‑1 (MQ=255)
     ttaaagatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatcgcaacgaagctaccttatggacacccgaagagcagattgatcaaaaaatttaccgcactagacccgtatattcgtgaagggaAGTgaaaac                                                                                                                                        >  1:503175‑M2/141‑148 (MQ=255)
      taaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGa                                                                                                                                       <  1:979665‑M2/10‑1 (MQ=255)
              tgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAt                                                                                                                               <  1:978599‑M2/18‑1 (MQ=255)
                ctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTc                                                                                                                             <  1:929610‑M2/20‑1 (MQ=255)
                            aatttggtaaatcaatcgcatcacaacagaagatagcaatgacgtaaccgagtggacacccggagagcagattgatcaaaaaattcaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGt                                                                                                                 >  2:1032238‑M2/118‑149 (MQ=255)
                            aatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttagcgcactaggcccgtatattcgtgaatgtaAGTGCAAAGATAATCGATTCTTTTTCgcttat                                                                                                                 <  2:996615‑M2/32‑3 (MQ=255)
                               gtggtaaaacagttgcatcacgacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcacttggcccgtatattcgggaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTg                                                                                                              <  2:351225‑M2/35‑1 (MQ=255)
                                tggtaaaacggttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                             <  1:165117‑M2/36‑1 (MQ=255)
                                  gcaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATTATCGATTCTTTTTCGATTGTCTGGCt                                                                                                           <  1:438216‑M2/38‑1 (MQ=255)
                                                tcacaacaggagatagcaatgacgttatcgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                                                             <  1:87428‑M2/52‑1 (MQ=255)
                                                    aacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttccggcagtcggactgattacgcgtgagggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGGCAACGTcaac                                                                                         >  2:70‑M2/94‑148 (MQ=255)
                                                            atagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCTATTTTCTGGCTGTATAGATCAACGTGAAACCGGCAcc                                                                                 >  2:570800‑M2/86‑149 (MQ=255)
                                                                 aatgacgttaccgagtggacacccgcacagccgtctgatcaaaaaagttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                                                            >  2:532604‑M2/81‑149 (MQ=255)
                                                                  atgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                                                           >  2:190926‑M2/80‑149 (MQ=255)
                                                                  atcgcgttaccgaggtgacaccggaagagcagattgatcaaaaaatttacggcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                                                           <  2:1084608‑M2/70‑1 (MQ=255)
                                                                    gacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGc                                                                         <  1:252120‑M2/72‑1 (MQ=255)
                                                                      cgttaccgagtggacacccgaagagaagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                       <  1:138467‑M2/74‑1 (MQ=255)
                                                                      cggtaccgatgggacatccgaatagcatattgatcaaaaaatttaccgcatcaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                       <  2:493807‑M2/74‑1 (MQ=255)
                                                                          accgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAt                                                                   >  2:498598‑M2/72‑149 (MQ=255)
                                                                             gagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAGTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCt                                                                >  1:698135‑M2/69‑149 (MQ=255)
                                                                             gagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgagaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTATATGCGTCAGCGTGATAGCGACACCTGAAGTAATTGAATTCt                                                                >  2:571565‑M2/69‑149 (MQ=255)
                                                                              agtggacacccgaagagtagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                                                               <  1:177516‑M2/82‑1 (MQ=255)
                                                                              agtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                                                               <  1:689565‑M2/82‑1 (MQ=255)
                                                                                tcgacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTggg                                                             <  1:70‑M2/84‑1 (MQ=255)
                                                                                 ggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTTGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                                                            <  2:759518‑M2/85‑1 (MQ=255)
                                                                                  gacagccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGc                                                           >  1:38873‑M2/64‑149 (MQ=255)
                                                                                              gcaaattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAg                                               <  1:100786‑M2/98‑1 (MQ=255)
                                                                                                 gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTg                                            >  1:784046‑M2/49‑149 (MQ=255)
                                                                                                  attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTgg                                           <  1:54083‑M2/102‑1 (MQ=255)
                                                                                                     gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAg                                        <  1:481850‑M2/105‑1 (MQ=255)
                                                                                                       tcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcg                                      >  1:93080‑M2/43‑149 (MQ=255)
                                                                                                       tcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCGGGAAgcg                                      >  1:1101784‑M2/43‑149 (MQ=255)
                                                                                                        caaaaattttaccgacctaggccggtttattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGCAAgcgc                                     <  2:273553‑M2/108‑1 (MQ=255)
                                                                                                        caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcgc                                     <  2:784046‑M2/108‑1 (MQ=255)
                                                                                                         aaacaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                        <  1:792242‑M2/25‑1 (MQ=255)
                                                                                                         aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                        >  2:792242‑M2/41‑65 (MQ=255)
                                                                                                           aaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAgg                                  >  1:363891‑M2/39‑149 (MQ=255)
                                                                                                                ttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATcc                             <  2:308513‑M2/116‑1 (MQ=255)
                                                                                                                 gtccgcattaggcccggatattcgagaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTTGCTGTATGCGTCAACGTGAAATCGGCACCGGAAGTGCGTGAATTCTGTGGCTGGTGGATGGAGCTGGAAGAGCAGGGATccc                            <  2:649590‑M2/117‑1 (MQ=255)
                                                                                                                   ccgcatcaggccggtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGt                          <  2:65908‑M2/119‑1 (MQ=255)
                                                                                                                    cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGGATCCCGtt                         <  1:532604‑M2/120‑1 (MQ=255)
                                                                                                                      cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                        <  1:82496‑M2/25‑1 (MQ=255)
                                                                                                                      cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                        >  2:82496‑M2/28‑52 (MQ=255)
                                                                                                                      cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                        >  2:84008‑M2/28‑52 (MQ=255)
                                                                                                                      cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                        <  1:84008‑M2/25‑1 (MQ=255)
                                                                                                                        ctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                     <  1:116650‑M2/124‑1 (MQ=255)
                                                                                                                             cacgtctattcctgaagggaAGTCAAAAGATAATCGATTCTTTTTCGATGGTCTGGCTGTATGCGTCAACGTGAACACGGCACCGGCAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa                <  1:539002‑M2/129‑1 (MQ=255)
                                                                                                                                 tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggt                                                       >  1:736152‑M2/17‑106 (MQ=255)
                                                                                                                                 tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggt                                                       <  2:736152‑M2/90‑1 (MQ=255)
                                                                                                                                 tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTa            >  1:574653‑M2/17‑149 (MQ=255)
                                                                                                                                   tattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATGCCGTTTTACCTACAGTTAcc          >  1:189705‑M2/15‑149 (MQ=255)
                                                                                                                                    attcgtgaaggtaAGTGCAATGATAATCGATTCTTTTTCGATTGTCTGGc                                                                                                            <  2:1034209‑M2/37‑1 (MQ=255)
                                                                                                                                    attcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                                                            >  1:1034209‑M2/14‑50 (MQ=255)
                                                                                                                                     ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggt                                                       <  1:759633‑M2/90‑1 (MQ=255)
                                                                                                                                     ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggt                                                       >  2:759633‑M2/13‑102 (MQ=255)
                                                                                                                                        gggaatgtaAGTGAAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGttt     <  2:853424‑M2/140‑1 (MQ=255)
                                                                                                                                         ttaaggtaAGTTCAAAGATAATTGATTATTTTTGGAGTGTCTGGCTGTATGGGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg    <  1:317795‑M2/141‑1 (MQ=255)
                                                                                                                                         tgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg    <  1:570800‑M2/141‑1 (MQ=255)
                                                                                                                                           aaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGt  <  2:1009551‑M2/143‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
GATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGT  >  NC_000913_3_pae_tpiA/258539‑258826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.