Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 482,230 G→A P725L (CCG→CTG)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA482,2300GA98.4% 211.9 / ‑3.8 65P725L (CCG→CTG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (36/27);  minor base C (1/0);  total (37/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

TGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCG  >  NC_000913_3_pae_tpiA/482084‑482370
                                                                                                                                                  |                                                                                                                                            
tgacttAAACTTTCTTCACACGACGGCGGTCTATAAAGTCGTTCACATAGCTGCCGCCCCCTGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTTGTCGATATCAATCTTAAACTGCagg                                                                                                                                            <  1:561630/149‑1 (MQ=255)
  acatAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAAGGTGGTCTTCACATTGGTGTCATAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGt                                                                                                                                          <  1:1122892/149‑1 (MQ=255)
    atAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCCATCTTAGACTGCAGGGTag                                                                                                                                        >  1:335866/1‑148 (MQ=255)
    atAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCACCGCCCAGCACGGTGGTAATGTCGTTGATAGAAACACCCAGCCCCTGCGCTTTTTCCTGGCCGCAACCAATCTCAAACTCCAGGGTac                                                                                                                                        >  1:158567/1‑148 (MQ=255)
        aCTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                    <  1:737377/149‑1 (MQ=255)
        aCTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                    >  2:358967/1‑149 (MQ=255)
         cTTTCCTCACACCACCGCGGTCGATAAAGTCGTCCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCCCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTcc                                                                                                                                   <  1:372763/149‑1 (MQ=255)
          tttCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCa                                                                                                                                  <  1:797661/149‑1 (MQ=255)
                  cacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGttt                                                                                                                          <  1:171396/149‑1 (MQ=255)
                  cacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGttt                                                                                                                          >  2:888338/1‑149 (MQ=255)
                     gACCGCGGGCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGa                                                                                                                       <  1:870705/149‑1 (MQ=255)
                        cgcgGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGCCGt                                                                                                                    >  1:638380/1‑149 (MQ=255)
                               gATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTgg                                                                                                             >  1:1068219/1‑149 (MQ=255)
                                    aGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCCTTTTCCTTGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAAc                                                                                                        <  1:123255/149‑1 (MQ=255)
                                    aGTCGGTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCAAGCGCCTGCGCTTTTTAGTTGTGGATGTCATTCTTTCCCGTGTGGGTATCTTCCAGACCGTTTGGACGGACGCTGGTCAAc                                                                                                        <  2:1096677/149‑1 (MQ=255)
                                            acaTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTCATGTCGTTGATAGAAACACCCAACGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGGCAACATATCAgg                                                                                                >  1:1025300/1‑149 (MQ=255)
                                            acaTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAgg                                                                                                >  1:399016/1‑149 (MQ=255)
                                             caTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAggg                                                                                               <  1:1085579/149‑1 (MQ=255)
                                              aTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGCTATCTTCCGAACCGTTTGTACGTACGCTGGTCACCATATCAGGGt                                                                                              >  2:49366/1‑149 (MQ=255)
                                                        ccccATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGAGGCTGCGCGTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgctgc                                                                                    >  1:1076443/1‑149 (MQ=255)
                                                                 gcgcCCAGAGTGGTTTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaag                                                                           <  2:653710/149‑1 (MQ=255)
                                                                 gcgcCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGCGTGCTTCGCTGCTTCTgcaag                                                                           >  2:39123/1‑149 (MQ=255)
                                                                     ccAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAAc                                                                       >  1:198728/1‑149 (MQ=255)
                                                                              tggtAAGGTAGTTGAGAGGTACACCCAGCGCCTGCGGTTGTTCCTGGTGGATATCACTCTTAAACTGCAGGGTAGCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTAcgc                                                              <  2:21383/146‑1 (MQ=255)
                                                                               gtTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTAcgcg                                                             >  1:394557/1‑149 (MQ=255)
                                                                                ttAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAAATGGTTACgcgc                                                            >  1:18979/1‑149 (MQ=255)
                                                                                  aaTGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCt                                                          >  1:1135213/1‑149 (MQ=255)
                                                                                  aaTGCCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCt                                                          <  2:946431/149‑1 (MQ=255)
                                                                                      tCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGa                                                                                                                       <  1:808812/84‑1 (MQ=255)
                                                                                      tCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGa                                                                                                                       >  2:808812/1‑84 (MQ=255)
                                                                                      tCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGCGTGCTTCGCTCCTTCTGCACGCACCTGGTTACGCGCATgcgc                                                      >  2:737018/1‑146 (MQ=255)
                                                                                        gTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAggg                                                                                               >  1:697773/1‑106 (MQ=255)
                                                                                        gTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAggg                                                                                               <  2:697773/106‑1 (MQ=255)
                                                                                           gATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGtt                                                 >  1:576437/1‑149 (MQ=255)
                                                                                            aTAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGATTCAtttt                                                >  2:570919/1‑149 (MQ=255)
                                                                                               gAAACTCCCAGCGCCTTCTCGTTTTCCTGTTCGATATTAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCGGGTCAACATAGCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                             <  2:768118/149‑1 (MQ=255)
                                                                                               gAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                             <  1:1094320/149‑1 (MQ=255)
                                                                                                aaaCCCCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                            <  1:811668/149‑1 (MQ=255)
                                                                                                aaaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                            >  1:346053/1‑149 (MQ=255)
                                                                                                 aaCAGCCAGCGTGTGCGGTTTTTCCTGGTCGATATCACTCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGGACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                           <  2:605284/149‑1 (MQ=255)
                                                                                                 aaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGGAGGGTATCTTCCAGACCCTTTGGACGTACGCTGGTCACCATATCAGGGTGCTTCCCTGCTTCTGCAAGCATCTGGTTACGCGCTTCAGTCAGTTTTTCGt                                           <  2:1032488/149‑1 (MQ=255)
                                                                                                  acacCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTg                                          >  1:76165/1‑149 (MQ=255)
                                                                                                  acacCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTg                                          >  1:96288/1‑149 (MQ=255)
                                                                                                  acacCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCGCAGTCAGTTTTTCGTg                                          >  2:132237/1‑149 (MQ=255)
                                                                                                      ccAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCa                                      >  1:775286/1‑149 (MQ=255)
                                                                                                      ccAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCa                                      >  1:257136/1‑149 (MQ=255)
                                                                                                            gcggcgcTTTTTCCTGGCCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCa                                <  2:381042/146‑1 (MQ=255)
                                                                                                             cTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAg                               <  1:888338/149‑1 (MQ=255)
                                                                                                              tGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAg                               >  2:145845/1‑148 (MQ=255)
                                                                                                              tGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAg                               <  1:145845/148‑1 (MQ=255)
                                                                                                                cgcTGTTTTCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCt                            <  2:950376/149‑1 (MQ=255)
                                                                                                                 gcTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTAAGCTGGTAACCATATCAGGGTGATTCGCTGCTTCTGCAAGCAACTCGTTCGGCGCCTGAGCCAGTTTTTAGTGACCATGGCCAGCCTg                           >  1:234266/1‑149 (MQ=255)
                                                                                                                  cTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTTCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTgg                          >  2:498553/1‑149 (MQ=255)
                                                                                                                         cTGGTCGATATCAATCTTAAACTGACGCGTATCTTCCAGACCGTTTGGAAGTACGCTGGTCAACCTATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCa                   >  1:274980/1‑149 (MQ=255)
                                                                                                                           ggTCGATATCAATCTTAAACTGCAGGGTATCCTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTGTTCGTGACCAAGGCCAGCCTGGTCAATCAGc                 <  1:583955/149‑1 (MQ=255)
                                                                                                                            gTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaagcaa                                                                        <  2:579137/93‑1 (MQ=255)
                                                                                                                            gTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaagcaa                                                                        >  1:579137/1‑93 (MQ=255)
                                                                                                                             tCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtc               >  1:286738/1‑149 (MQ=255)
                                                                                                                                  atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagt          <  1:878104/149‑1 (MQ=255)
                                                                                                                                     aaTCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGAc                                                                                                                      >  2:490285/1‑38 (MQ=255)
                                                                                                                                     aaTCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGAc                                                                                                                      <  1:490285/38‑1 (MQ=255)
                                                                                                                                       tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCACCATGTCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag     <  2:335866/149‑1 (MQ=255)
                                                                                                                                       tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCCGCtcaaagtaaaag     >  1:10106/1‑149 (MQ=255)
                                                                                                                                         ttAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc   >  1:47420/1‑149 (MQ=255)
                                                                                                                                         ttAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc   >  1:940694/1‑149 (MQ=255)
                                                                                                                                          tAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCg  >  1:754792/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
TGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCG  >  NC_000913_3_pae_tpiA/482084‑482370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: