New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 2155287 =37 (1.010)4 (0.110) 4/272 4.8 10.8% coding (25/3123 nt) mdtB multidrug efflux system, subunit B
?NC_000913_3_pae_tpiA 2155309 = 31 (0.890)coding (47/3123 nt) mdtB multidrug efflux system, subunit B
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCCCTGCCCGTTTCGGCGCTGCCGGAAGTGGACTATCCGACCATTCAGGTG  >  NC_000913_3_pae_tpiA/2155140‑2155430
                                                                                                                                                   |                                                                                                                                               
gACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGAGCCACTACTACGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGcac                                                                                                                                                >  1:199793/1‑149 (MQ=255)
gACAGACGGCATTGACCGCCTGACCGAAGGGGCCAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGcac                                                                                                                                                <  2:550370/149‑1 (MQ=255)
   aGACGGCATTGATCTCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCAAGAGCGCCACTACTCCGCAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAgg                                                                                                                                             <  1:595039/149‑1 (MQ=255)
    aaCGGCATGCATCGCCTGAACGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCAGCGAAAAAACGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAggc                                                                                                                                            <  2:589881/148‑1 (MQ=255)
     aCGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAggcg                                                                                                                                           >  1:17421/1‑149 (MQ=255)
      cGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGTAACACGCTCCTAATGCAGGTGTTACCCCCGAGCAGCACAggcgg                                                                                                                                          >  1:87004/1‑149 (MQ=255)
               tCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTcgcg                                                                                                                                 >  1:589015/1‑149 (MQ=255)
                        cGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACCACTCCGGAAGAGAAAGCCACCAGCCGCGAAGCCGCGAAAAAAGCAGCACGCTCATAATACGGGTGTTACCCCCGGCCCGCCAAGGCGCCCCGTCGCGCCTGcttgt                                                                                                                        >  2:158208/1‑147 (MQ=255)
                         gAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTttatt                                                                                                                       <  2:259220/149‑1 (MQ=255)
                          aaGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCCCAGGCGGCCCTTCGCGCCCGtatctaa                                                                                                                      >  2:286107/1‑143 (MQ=255)
                                cGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTc                                                                                                                <  2:17421/149‑1 (MQ=255)
                                  aaaGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCGACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCGCGTCGCGCCTGTTTATTATGCGTccg                                                                                                              >  1:326518/1‑148 (MQ=255)
                                              gtggAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGAACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCCGTTGCCACCACg                                                                                                  >  2:119831/1‑149 (MQ=255)
                                                ggAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACgct                                                                                                >  2:536948/1‑149 (MQ=255)
                                                ggAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACgct                                                                                                <  1:536948/149‑1 (MQ=255)
                                                 gAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACgctg                                                                                               >  1:250923/1‑149 (MQ=255)
                                                      ccAGAGCGCCACTACTCCGGAAGATAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCGCGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGAt                                                                                          <  1:610454/149‑1 (MQ=255)
                                                           gcgcCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCGGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGAtggtgg                                                                                     <  1:616721/149‑1 (MQ=255)
                                                              ccACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGa                                                                                  >  2:518392/1‑149 (MQ=255)
                                                               cACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGCGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGAt                                                                                 >  1:551827/1‑149 (MQ=255)
                                                                actactCCGGAAGGGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATc                                                                                <  2:452282/149‑1 (MQ=255)
                                                                   tacccGGAAGAGAAAGCCACCGGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTa                                                                             <  2:551827/146‑1 (MQ=255)
                                                                   actCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTa                                                                             <  1:524780/149‑1 (MQ=255)
                                                                     tCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACt                                                                           >  2:429918/1‑149 (MQ=255)
                                                                        ggAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGc                                                                        >  1:335830/1‑149 (MQ=255)
                                                                            gagaAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCggg                                                                    >  1:108644/1‑149 (MQ=255)
                                                                                    caccaGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCgg                                                            <  1:433050/149‑1 (MQ=255)
                                                                                                 tACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCCCTg                                               <  1:518392/149‑1 (MQ=255)
                                                                                                       aaaaaaGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCACTGCCCGtt                                         >  1:451434/1‑149 (MQ=255)
                                                                                                             ggAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGGCGCGCCTGTGTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCCCTGCCCGTTTCGgcg                                   <  1:66811/149‑1 (MQ=255)
                                                                                                                        cTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGGCCGTCGCTCCGGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCCCTGCCCGTTTCGGCGCTGCCGGAAGt                        <  1:178277/149‑1 (MQ=255)
                                                                                                                          gATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCCCTGCCCGTTTCGGCGCTGCCGGAAGTgg                      >  1:586188/1‑149 (MQ=255)
                                                                                                                           ctccAGGTGTTACCCCCGAGCGCGGCAGGCGGCACGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCACGCCCTGTTTGTTTCGGCGCTGCCGGAAGTGGa                     >  2:457588/4‑149 (MQ=255)
                                                                                                                           aTGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCCCTGCCCGTTTCGGCGCTGCCGGAAGTGGa                     <  1:585835/149‑1 (MQ=255)
                                                                                                                                        ccccGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCCCTGCCCGTTTCGGCGCTGCCGGAAGTGGACTATCCGACCAtt        >  1:450539/1‑149 (MQ=255)
                                                                                                                                             agcagcACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCCCTGCCCGTTTCGGCGCTGCCGGAAGTGGACTATCCGACCATTCCggt   >  1:117533/1‑149 (MQ=255)
                                                                                                                                              gcagcaCAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCCCTGCCCGTTTCGGCGCTGCCGGAAGTGGACTATCCGACCCTTCAggtg  >  1:467939/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCCCTGCCCGTTTCGGCGCTGCCGGAAGTGGACTATCCGACCATTCAGGTG  >  NC_000913_3_pae_tpiA/2155140‑2155430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.