New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 336528446 (1.440)5 (0.160) 5/272 3.6 10.4% pseudogene (100/367 nt) yhcE pseudogene fragment; interrupted by IS5
?NC_000913_3_pae_tpiA = 3365294 42 (1.370)pseudogene (110/367 nt) yhcE pseudogene fragment; interrupted by IS5
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGATT  >  NC_000913_3_pae_tpiA/3365145‑3365432
                                                                                                                                           |                                                                                                                                                    
cAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAACtg                                                                                                                                             >  2:330455/1‑149 (MQ=255)
cAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGAGGCCTGCTGCTGAACTTCGCCGTGGCCGCTCAGGAGGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGtc                                                                                                                                             >  2:356529/1‑148 (MQ=255)
cAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGAAGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGGGTAATATCTCCAGTCGCATACAAAATATCGACTATGGCAAACGAAGtg                                                                                                                                             >  2:330739/1‑149 (MQ=255)
          gtTCTATGAAACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGAAAACCAAATGCTGACATacg                                                                                                                                   >  2:156624/1‑149 (MQ=255)
           ttCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATgcgc                                                                                                                                  >  2:165099/1‑149 (MQ=255)
                        cAACAGGCGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGCAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCggg                                                                                                                     <  2:66430/149‑1 (MQ=255)
                        cAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGCGATTTGCCATGGCTACTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCggg                                                                                                                     >  2:65119/1‑149 (MQ=255)
                         aaCAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCgggg                                                                                                                    >  1:272588/1‑149 (MQ=255)
                           cAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTAACCGGGGTa                                                                                                                  >  2:3856/1‑149 (MQ=255)
                             ggAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGGTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCACCTATGGCAAACGAAGTGCAGCAATGCGCCAAGTTAACCggcgaaca                                                                                                                >  2:34976/1‑143 (MQ=255)
                                   tcccAATGAAACGGACAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAacaaca                                                                                                          <  1:172569/147‑1 (MQ=255)
                                   tGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAacaaca                                                                                                          <  1:165099/149‑1 (MQ=255)
                                     ccAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGGCTATGGCAAACGAAGTGCTGCCATACGCCAAGTTGACCGGGGTAAAACAAcaca                                                                                                        >  1:489715/1‑149 (MQ=255)
                                      cAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACaa                                                                                                       >  2:327297/1‑149 (MQ=255)
                                       aaTGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAAcacact                                                                                                      >  2:139299/1‑147 (MQ=255)
                                             aCGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCGGCCTTGCGCAAAGCTGACGGGGGTAAAACAACCAAATTAGcag                                                                                                >  2:91131/1‑149 (MQ=255)
                                             aCGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGCGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTCCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTCGcag                                                                                                >  2:424589/1‑149 (MQ=255)
                                              cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGcaga                                                                                               <  1:101193/149‑1 (MQ=255)
                                                   aatacgcggATGCCTGCTGCTGAACCTCGCCATGGCCGCTCCGGCAGCGTGTCCTCTCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGaa                                                                                          <  2:391704/142‑1 (MQ=255)
                                                   aataaCAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGaa                                                                                          <  1:383477/149‑1 (MQ=255)
                                                     taaCAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAAACAGAACa                                                                                        >  2:261741/1‑149 (MQ=255)
                                                        cAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGATAACCGGGGTAAAACAACACAATTAGCGGACAGaaaactc                                                                                     >  2:435239/1‑144 (MQ=255)
                                                         aGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCa                                                                                    >  1:409923/1‑149 (MQ=255)
                                                           gATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACg                                                                                  >  1:174153/1‑149 (MQ=255)
                                                            aTGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGc                                                                                 >  2:143284/1‑149 (MQ=255)
                                                                ctgctgctgAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATAGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCAGGCTGGt                                                                             >  2:340109/1‑149 (MQ=255)
                                                                  gctgctgAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTaa                                                                           <  1:569716/149‑1 (MQ=255)
                                                                    tgctgAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTaatg                                                                         <  1:508976/149‑1 (MQ=255)
                                                                       tgAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTaatgcaa                                                                      >  2:451811/1‑149 (MQ=255)
                                                                        gAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTaatgcaat                                                                     <  1:327297/149‑1 (MQ=255)
                                                                         aaCTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGt                                                                                                                                              >  1:36283/1‑75 (MQ=255)
                                                                         aaCTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGt                                                                                                                                              <  2:36283/75‑1 (MQ=255)
                                                                            ttCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGa                                                                 >  1:195961/1‑149 (MQ=255)
                                                                            ttCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATCCGa                                                                 >  1:122943/1‑149 (MQ=255)
                                                                               gCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCa                                                              <  1:497050/149‑1 (MQ=255)
                                                                                ccATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAg                                                             >  1:285373/1‑149 (MQ=255)
                                                                                           cAGGCAGAGTATAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTACCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCatat                                                  <  1:65119/149‑1 (MQ=255)
                                                                                              gCAGAGTATAATATCTCCAGTAGCATACAAAATATCGACTATCGCAAACGAAGTGCTACCATGCAACAAGTTCACCGGGGGAAAAAAAGATAATTAACAGGCAGAACAATCACGCCGGCAAAGCAATGAGATAAGGACGCTCATATTCg                                               >  2:335677/1‑149 (MQ=255)
                                                                                               cAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCgt                                              <  1:517067/149‑1 (MQ=255)
                                                                                                agacTGTAATATCTCCAGGAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTTATATTCgtg                                             <  1:241349/149‑1 (MQ=255)
                                                                                                        atatCTCCAGTAGCATACAAACTATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACTCGGGTAAGGCAATGCGATCAGGACGCGCATATTCGGGTTCAGTTg                                     >  2:501735/1‑149 (MQ=255)
                                                                                                               cAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCg                              <  1:451811/149‑1 (MQ=255)
                                                                                                                      aTACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAatat                       >  1:2847/1‑149 (MQ=255)
                                                                                                                         cAACATATCGACCATGGCAAACGCAGTGCTACCATGCGCCAAGTTGCACGGGGTAAAACAACACAATTAGCAGACAGAACCATCCCGCTGGTAATGCAATGCTATCAGAACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATATc                    <  2:7509/149‑1 (MQ=255)
                                                                                                                              tatCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCcaaca               >  1:471484/1‑149 (MQ=255)
                                                                                                                                   accaaggCAAACCACGTGCTGCCATGCGCCAAGATGACCGTGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACgga          <  1:242830/144‑1 (MQ=255)
                                                                                                                                           aaaCGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACggatttggatt  >  2:368434/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
CAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGATT  >  NC_000913_3_pae_tpiA/3365145‑3365432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.