New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	644022 =	33 (1.030)	6 (0.200)	5/268	3.6	17.6%	intergenic (‑55/+175)	rnk/rna	regulator of nucleoside diphosphate kinase/ribonuclease I
	?	NC_000913_3_pae_tpiA	644051 =	25 (0.830)					intergenic (‑84/+146)	rnk/rna	regulator of nucleoside diphosphate kinase/ribonuclease I
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCGTGTTTTGTAAATCAGAT  >  NC_000913_3_pae_tpiA/643907‑644194                                                                                                                                                 |                                                                                                                                                ccAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTc                                                                                                                                             <  1:384200/149‑1 (MQ=255)            gcggggCGTGCGGTATCCAGGTCGTTAGTGATGATAGTTGGTCTGGACAGTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGTTa                                                                                                                                  <  2:221405/144‑1 (MQ=255)                    ttCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGt                                                                                                                          >  1:438783/1‑149 (MQ=255)                       tgCACCCATGTCGTTAATGAGGGTTGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGc                                                                                                                       <  2:266521/148‑1 (MQ=255)                         cATCCAGGGCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTc                                                                                                                     <  2:371941/149‑1 (MQ=255)                           tCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAACCCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTccc                                                                                                                   >  1:345333/1‑147 (MQ=255)                             cAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGACAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTTACTGTTGCTCActc                                                                                                                 >  2:506282/1‑149 (MQ=255)                             cAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCActc                                                                                                                 >  2:199900/1‑149 (MQ=255)                                gTCGTTAATGATGATAGTTGGTCTGGACATTTTTCGTCCATGTCTTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCttt                                                                                                              <  1:531757/149‑1 (MQ=255)                                   gTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGaa                                                                                                           <  2:349939/149‑1 (MQ=255)                                       atgatgATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGa                                                                                                       >  2:536011/1‑149 (MQ=255)                                         gatgatAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGAtt                                                                                                     >  1:232402/1‑149 (MQ=255)                                          atgatAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGATTAACTCACATGATGATACTGACTGTTCCTCACTCTTTGAAGTGAttt                                                                                                    >  2:46135/1‑149 (MQ=255)                                                gTTGGTCTGGACATTTTTACTCCATGTCTTCTGTGCTGCGAGTGCCGCAGATAAACATACCCAAAAGAAACCCCTCACCGTCAGGCGGCGAGGGTTTAACTCCCATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTca                                                                                              <  1:118876/149‑1 (MQ=255)                                                        ggACATTTTTACTCCAGGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAgg                                                                                      <  1:69638/149‑1 (MQ=255)                                                                    tCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTGAGGGAAGTCCTCAAt                                                                          >  1:229362/1‑149 (MQ=255)                                                                             tcgGTGCTGCGAGTGTCGCAGATAAACATACCCCAAAGAAATCCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCAt                                                                 <  2:304671/149‑1 (MQ=255)                                                                                gtgtTGCGAGTGGCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTGTACCTCACATGATGATACTGACTGTTGCTCACTCTCTGACGTGATTTGCGTCACAGTCAGGGAATTCCCCGATTCAGCGCATTAt                                                              <  2:514890/149‑1 (MQ=255)                                                                                              gCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAAGTCCTCAATGCAGCGCATTGTGTATAAATCTTAAt                                                >  1:216272/1‑149 (MQ=255)                                                                                                  aTAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGcc                                            >  1:565107/1‑149 (MQ=255)                                                                                                               aaagcaaagcCTCACCGTTAGGCGGCGAGGGTTTAACTCACATGATGATCCTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTTCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGaa                               <  1:264294/149‑1 (MQ=255)                                                                                                                      aCCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAAt                        <  1:322250/149‑1 (MQ=255)                                                                                                                       cccTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAAtt                       <  1:199900/149‑1 (MQ=255)                                                                                                                                     cggcgAGGGTTTAACTCACATGAGGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCGTGTTTTGTaa         <  1:519723/149‑1 (MQ=255)                                                                                                                                       gcgAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCGTGTTTTGTAAAt       <  1:231238/149‑1 (MQ=255)                                                                                                                                            ggTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCGTGTTTTGTAAATCAgat  >  2:226438/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                CCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCGTGTTTTGTAAATCAGAT  >  NC_000913_3_pae_tpiA/643907‑644194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.