New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 333519956 (0.940)5 (0.090) 5/286 8.1 8.8% intergenic (+12/+36) sfsB/murA malPQ operon transcriptional activator/UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
?NC_000913_3_pae_tpiA = 3335219 49 (0.830)intergenic (+32/+16) sfsB/murA malPQ operon transcriptional activator/UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTC  >  NC_000913_3_pae_tpiA/3335051‑3335345
                                                                                                                                                    |                                                                                                                                                   
aaGGAGAGATGATTATTGCGAAAGCCCTTGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCgg                                                                                                                                                     >  2:242867/1‑149 (MQ=255)
      agaTGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGccc                                                                                                                                               >  2:868245/1‑149 (MQ=255)
       gattatTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGcccc                                                                                                                                             <  1:868245/149‑1 (MQ=255)
        atgatTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGAACCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTAAACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGcccca                                                                                                                                            >  2:547902/1‑148 (MQ=255)
            ttattGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGaa                                                                                                                                        <  1:515191/149‑1 (MQ=255)
              attGCGAAAGCCCTGGGAACTGACCTCTGGTTTATCTGGTCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACg                                                                                                                                      <  1:191763/149‑1 (MQ=255)
              attGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCAGAGAACg                                                                                                                                      >  1:525536/1‑149 (MQ=255)
                tGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCATACCCATGAGCTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACggg                                                                                                                                    <  1:963131/149‑1 (MQ=255)
                tGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACggg                                                                                                                                    >  2:165653/1‑149 (MQ=255)
                   gAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAATGGTCTGGCGGTAGCCCCGCGAACGGGGCt                                                                                                                                 >  2:145353/1‑149 (MQ=255)
                       gCCCTGGGAACTGACCCCTGGGTTATTTGGCTATCACGCTACCTTGATCCGCTGACTCATGAGTTTATCGACAGATCGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCa                                                                                                                             <  1:57902/149‑1 (MQ=255)
                          cTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGCACGGGGCTGCCAGct                                                                                                                          >  2:798625/1‑149 (MQ=255)
                           tGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGctc                                                                                                                         >  1:857469/1‑149 (MQ=255)
                             ggAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGGGGTCTGGCGGTAGACCCGCGAAAGGGGCTGGCAGccgtc                                                                                                                       >  2:1039797/1‑145 (MQ=255)
                                  tGACCCCTGGGTTATCTGGCCATCACGCTACCAGGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAGTGAGTGGTCTGGCGGTAGGCGCGCGAACGGGGCGACGAGCACTCCGACg                                                                                                                  >  1:841136/1‑149 (MQ=255)
                                  tGACCCCTGAGTTATCTGGCCATCACGCTACCATGATCCGCAGAACCATGAGTTTATCGACAGAACGCAGTTCATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGCGGCTGCCAGCTCTCAGAct                                                                                                                  >  2:474897/1‑148 (MQ=255)
                                      cccTGCGTTATCTGGCCATCACGCCACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGATGATGAGTAGCTACACTAAACCGAAAAAATGAGTGGTCTCGCGGTCGCCCCGCGGACGGGGCAGACAGCTCTCAGACGatta                                                                                                              >  2:567014/1‑149 (MQ=255)
                                       ccTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCCCTCAGACGagtat                                                                                                             >  2:703525/1‑149 (MQ=255)
                                         tGGGTTATCTGGCCATCACTCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACTCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTc                                                                                                           <  1:268953/149‑1 (MQ=255)
                                                    gCCATCACGCTACCATGATCCGCAACCCCATGGGATTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGACCGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCacac                                                                                                >  1:250520/1‑149 (MQ=255)
                                                       aTCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAACTATCAGACGATTATTCGCCTTTCACACGCt                                                                                             >  1:936667/1‑149 (MQ=255)
                                                            gCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCActa                                                                                        >  1:779792/1‑149 (MQ=255)
                                                              tACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCATCTCTCAGACGATTATTCGCCTTTCACACGCTCAATAtt                                                                                      >  1:716037/1‑149 (MQ=255)
                                                                 cATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGACCGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCCATATTTGc                                                                                   >  2:328671/1‑149 (MQ=255)
                                                                   tGATCCGCAGACCCATGAGTTTATCGGCAGAACGCAGTTGCTGCGTAGCTGGACTAAACCGAACTACTGAATGGTCTGGCGGAGCCCGCACGAACGGAGCGGCCAACTCTATGACGATTATTCGACTTTCAGATGGTCAGTAtttcggc                                                                                 >  2:523057/1‑145 (MQ=255)
                                                                   tGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCAc                                                                                 <  1:543749/149‑1 (MQ=255)
                                                                   tGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGAATATTCGCCTTTCACACGCTAAATATTTGCAc                                                                                 >  1:16838/1‑149 (MQ=255)
                                                                   tGATCCGCAAACCCATGAGTTTATCGACAAAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGAGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCAc                                                                                 <  1:343194/149‑1 (MQ=255)
                                                                   tGATCACCAGACCAATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGGCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCAc                                                                                 <  1:343415/149‑1 (MQ=255)
                                                                      ccggaaGACCCAGGAGTTTATCAACAGAACGCAGTGAAGGCGTAGCCACACTAAACCGAAAAAATGAGTGGTCTGGCGGAGGCCCCGCGAACGGGGCGGCCAGCTCTCAGACGATTATCCCCCTTTCACACGCTCCATATTTGCACCTa                                                                              <  2:250520/144‑1 (MQ=255)
                                                                         gCAGACCCATTAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAg                                                                           <  1:1039797/149‑1 (MQ=255)
                                                                                 aTGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGt                                                                   <  1:242867/149‑1 (MQ=255)
                                                                                   gAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGATCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCGGttt                                                                 >  2:1019609/1‑149 (MQ=255)
                                                                                    aGTTTACCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCAACCTGGCAATATTTGCACCTAAAGCGCGCAGTTTg                                                                <  2:485841/149‑1 (MQ=255)
                                                                                          tCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTAGTCGCCTTTCTCACGCTCAATATTTGCACCTAAAGCGAGCAGTTAGTAttaa                                                          >  2:570510/1‑147 (MQ=255)
                                                                                            gACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGATCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAAt                                                        >  1:618183/1‑149 (MQ=255)
                                                                                            gACAGAACGATGTTGATGCGTAGCTTCACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCCTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAAt                                                        <  1:595646/149‑1 (MQ=255)
                                                                                             aCAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATg                                                       >  1:129434/1‑149 (MQ=255)
                                                                                              ctaaaCGCAGTTGATGCGTAGCTACAGTAAACCGAAAAAATGAGTTGTCTGGCCGTAGACCCGCGAACGGGGCTGCCCGCTCTCAGACGATTATTCGCCTTTCACACGCGCAATATTTGCACCTCAAGCGCGCAGTTTGTCTTCAATGc                                                      <  1:60567/146‑1 (MQ=255)
                                                                                               aGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGCTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGAAGATTATTCGCCTTTCACACGCTCACTATTTGCACCGACAGCGCGCCGCTCGTCTTCCATGCg                                                     >  2:263523/1‑149 (MQ=255)
                                                                                                gAACGCAGTTGATGCGTAGCTACTCTGAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGt                                                    <  1:758510/149‑1 (MQ=255)
                                                                                                  aCGCAGTTGATGCTTAGCTACACTAAACCTAAAAAATGAGTTGTCTGACGGTACCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTc                                                  <  1:923114/149‑1 (MQ=255)
                                                                                                   cGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTAACAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCg                                                 >  1:66147/1‑149 (MQ=255)
                                                                                                     cAGTTGATGCGTAGCGACACAAAAACGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTa                                               <  2:978260/149‑1 (MQ=255)
                                                                                                           aTGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCAcg                                         >  1:685701/1‑149 (MQ=255)
                                                                                                             gCGTAGCTACACTAAATCAAAAAAATGATTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCAcgat                                       <  1:135966/149‑1 (MQ=255)
                                                                                                                    tACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGAtgtg                                >  1:786070/1‑149 (MQ=255)
                                                                                                                    tACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGCTCGTAGCCACGATCGAtgtg                                >  1:597595/1‑149 (MQ=255)
                                                                                                                      cacTAAACCGAAAAAATGAGTGGTCTGGCGGTACACCAGGGAATGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCACAGTTTGTCTTCAATGCGTTCTTAGCCACGATCGATGTGAt                              >  1:248619/1‑149 (MQ=255)
                                                                                                                         tAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATTTGAaaaa                           >  2:567327/1‑149 (MQ=255)
                                                                                                                            aCCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATAATTCGCCTTTCACACGCTCAATATTTGCCCCTAAAGCGCGCAGTTTGTCTTCAATGCGTTAGTAGCCACGATCGATGTGATAAATAc                        >  1:608900/1‑149 (MQ=255)
                                                                                                                              cGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGa                      >  2:750475/1‑149 (MQ=255)
                                                                                                                              cGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTACTGTTCTGGCACACGCCGCAGGTGTGCACCTAAAGCGCGCAGTGTGTCTTCAATGGGTTCGTAGCCACGATCGATGTGATAAATACGa                      >  1:1029213/1‑149 (MQ=255)
                                                                                                                                aaaaaaTGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCCCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATc                    <  2:896492/149‑1 (MQ=255)
                                                                                                                                              cTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATaaa                           <  2:375983/128‑1 (MQ=255)
                                                                                                                                              cTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATaaa                           >  1:375983/1‑128 (MQ=255)
                                                                                                                                                  cGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCTCCTTTCACACGTTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTc  <  1:512462/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AAGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGCGAACGGGGCTGCCAGCTCTCAGACGATTATTCGCCTTTCACACGCTCAATATTTGCACCTAAAGCGCGCAGTTTGTCTTCAATGCGTTCGTAGCCACGATCGATGTGATAAATACGATCAACCACCGTCGTCCCTTC  >  NC_000913_3_pae_tpiA/3335051‑3335345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.