New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	3525427 =	50 (0.840)	7 (0.130)	5/272	7.8	13.0%	intergenic (+4/+162)	mrcA/nudE	penicillin‑binding protein 1a, murein transglycosylase and transpeptidase/adenosine nucleotide hydrolase; Ap3A/Ap2A/ADP‑ribose/NADH hydrolase
	?	NC_000913_3_pae_tpiA	3525449 =	NA (NA)					intergenic (+26/+140)	mrcA/nudE	penicillin‑binding protein 1a, murein transglycosylase and transpeptidase/adenosine nucleotide hydrolase; Ap3A/Ap2A/ADP‑ribose/NADH hydrolase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAG  >  NC_000913_3_pae_tpiA/3525299‑3525573                                                                                                                                 |                                                                                                                                                   aGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGGGGCACAGGAATTGTTCTAATTAAAAATGCGCTTAGgggctctt                                                                                                                                >  2:685787/1‑145 (MQ=255)   ttAGCTAATGGTGGCAACAGCCGCGAAGAGTATTACATCGGAGGTACGCAGCCGACACAATAGGGAGTGAACGAGGTGGCAACGACCATTATCGATAATGGCCAGGCACAGGAATTGTTCTCATTAAAAAGGCGCTTCGGCGCCTTTTc                                                                                                                              >  2:163713/1‑149 (MQ=255)    tAGCTAATGGTGGCAACAGCCGCGAAGAGTATTCCCTCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCa                                                                                                                             <  1:503840/149‑1 (MQ=255)           tggtggCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCt                                                                                                                      >  2:107901/1‑149 (MQ=255)                    cAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTg                                                                                                             <  1:107901/149‑1 (MQ=255)                      gCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCa                                                                                                           <  1:141671/149‑1 (MQ=255)                         gcgAAGAGTATTGCATCGGAGGTACGCAGGTGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACtt                                                                                                        <  2:759085/149‑1 (MQ=255)                           gAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGt                                                                                                      >  1:803325/1‑149 (MQ=255)                             agagTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGCCAAAGTGCACTTGttt                                                                                                    >  2:161677/1‑149 (MQ=255)                                   tttCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAg                                                                                                                            >  2:691137/1‑119 (MQ=255)                                   tttCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAg                                                                                                                            <  1:691137/119‑1 (MQ=255)                                   tttCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGACg                                                                                              >  1:223535/1‑149 (MQ=255)                                   tttCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTCCGACGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGcag                                                                                              >  1:399398/1‑147 (MQ=255)                                   tttCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCACTTCGGCGCCGTTTCAGTTTACTAACAAAGTGCACTTGTTTATGCCg                                                                                              >  1:370230/1‑149 (MQ=255)                                   tttCATCGAAGGTACGCAGCCGACACAACAGACAGTGCACGAGGAGGAAACGACCAATATCGATAATGGAGAGGCAAAGGAATTGTACTGATTAAACAGGCACTTCGGCGCGTTTTCAGTTTGCTGACAAGGTGCCATTGTTTATTCCg                                                                                              >  2:550336/1‑149 (MQ=255)                                      cATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGAt                                                                                           >  1:681540/1‑149 (MQ=255)                                           aaGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTTGGAACGACTATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGc                                                                                      <  1:289124/149‑1 (MQ=255)                                                    aGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTCCAAAGGCGGTTCGCCGCTTCTTCCACTGAGTGACGAAGTGCACTTGTTTATGCCGGATACGGCGTGAAcgcg                                                                             <  2:219879/149‑1 (MQ=255)                                                    aGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAAcgcg                                                                             >  1:880159/1‑149 (MQ=255)                                                        gACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTAt                                                                         >  1:527372/1‑149 (MQ=255)                                                             aacaGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCGTGGTACGGCGTGGACGCGTTGTCCGGc                                                                    >  2:777039/1‑149 (MQ=255)                                                             aacaGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGc                                                                    >  2:34009/1‑149 (MQ=255)                                                             aacaGGCAGTGCACGAGGGGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTGCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGc                                                                    <  1:576097/149‑1 (MQ=255)                                                                 ggCAGTGCACGAGGTGGGAACGACCATTATCGATAATGCCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTTAACGCGTTATCCGGCAAAc                                                                >  2:362008/1‑149 (MQ=255)                                                                          cGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTg                                                       >  2:937125/1‑149 (MQ=255)                                                                            aGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGaa                                                     <  2:992837/149‑1 (MQ=255)                                                                               tGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAAc                                                  >  1:599552/1‑149 (MQ=255)                                                                                  gAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGACGGATACGGCGTGGACGCGTTATCCGGCTAACACAACCGAGAAAACTcc                                               >  2:426567/1‑148 (MQ=255)                                                                                   aaCGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCaa                                              >  1:61710/1‑149 (MQ=255)                                                                                      gACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAAGAGCTTCGGCGCCTTTTAAGGTGGCTGATAAAGTGCACTTGTTTATAACGGTTACGGCTTGAACACGTTATCCGGCCAATAAAATCGGGAAACGTCCATaa                                           >  2:1006799/1‑149 (MQ=255)                                                                                       aCCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATaaa                                          >  1:120262/1‑149 (MQ=255)                                                                                         cATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAAtt                                        >  2:332716/1‑149 (MQ=255)                                                                                          aTTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGACCGCGTTATCCGGCTAACAAAATCGTGCAACCTCAATAAATTg                                       >  1:998492/1‑149 (MQ=255)                                                                                           ttATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAGGGCGCGTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATACCGGATACGTCGTGAACGCGTTATCCGGCTAAACAAGTCGTGGAAACTCAAGAAATTga                                      >  2:582510/1‑148 (MQ=255)                                                                                           ttATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGc                                      >  1:420598/1‑149 (MQ=255)                                                                                              tgggtAATGGCGAGGCAGAGGAATTGTTCTTATTAAAAAGGCGCTTCGGCGCCTTTGCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGTATAAGGTGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAgc                                   <  2:455976/145‑2 (MQ=255)                                                                                              tCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGa                                   >  2:68573/1‑149 (MQ=255)                                                                                                gATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCGAACAAAATCGTGAAAACTCAATAAATTGCAGaaa                                 >  1:544955/1‑149 (MQ=255)                                                                                                     tGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCa                            <  1:332716/149‑1 (MQ=255)                                                                                                           ggCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGcc                      <  1:161677/149‑1 (MQ=255)                                                                                                            gCACAAGAATTGTTCTGATTAAAAAGGCCATTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCt                     <  1:913920/149‑1 (MQ=255)                                                                                                             cacaGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTg                    <  1:68573/149‑1 (MQ=255)                                                                                                              acaGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCAGATACGGCGTGAACGCGTTGTCCGGCTAACAAAATCTTGAAAACTCAATAAATTGCGGAAACCCAATAAGCCtac                   >  2:716872/1‑147 (MQ=255)                                                                                                                       gTTCTGATTAAAAAGGTGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATtg          <  1:554211/149‑1 (MQ=255)                                                                                                                        ttCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCa                                     >  2:797767/1‑121 (MQ=255)                                                                                                                        ttCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCa                                     <  1:797767/121‑1 (MQ=255)                                                                                                                            gATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGGTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACACAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCAt     >  2:826163/1‑149 (MQ=255)                                                                                                                            gATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTCTGCAt     >  2:806511/1‑149 (MQ=255)                                                                                                                               tAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCGGATAAACATTGTGTATCAg  <  2:880159/149‑1 (MQ=255)                                                                                                                               caaaaaCGCGCTTCGGCGCCTTTTCATTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGATCGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATATATTGCAGAAACCCCATAAGCCTGATAAACATCGTGCATctg  <  2:71312/148‑3 (MQ=255)                                                                                                                                 |                                                                                                                                                   AGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAG  >  NC_000913_3_pae_tpiA/3525299‑3525573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.