New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	1552011 =	70 (1.170)	5 (0.090)	5/274	7.8	7.0%	intergenic (+20/+387)	fdnI/yddM	formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
	?	NC_000913_3_pae_tpiA	1552221 =	NA (NA)					intergenic (+230/+177)	fdnI/yddM	formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGT  >  NC_000913_3_pae_tpiA/1552075‑1552359                                                                                                                                                   |                                                                                                                                           taTGCGGAGGGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCTTCATTTTCatat                                                                                                                                          <  2:448609/148‑1 (MQ=255)            aaCGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACg                                                                                                                               >  2:291759/1‑149 (MQ=255)             aCGTCTTGTGCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGACTGATAAGCGTAGAGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGCGGTCATTTTCTTATTGTAGACAAAGt                                                                                                                              >  2:442749/1‑149 (MQ=17)                 cTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGc                                                                                                                          >  1:364411/1‑149 (MQ=255)                  ttATCCGGCATGAAAACCCTTCAAATCCAAAAGATTACGTTGAACGTGTAGGCCTGATAAGCGTAGCGAATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAAGCGTCATTTTCATATGTTAGACAACGTAGGCt                                                                                                                         >  2:747183/1‑149 (MQ=17)                    aTCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCttt                                                                                                                       <  2:116998/149‑1 (MQ=255)                    aTCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCttt                                                                                                                       >  1:414840/1‑149 (MQ=255)                        ggcaataaaaCACTTAAATCCCAATAGATTGCAGTGAACGGGTAGGCCCGATAAGCGTAGCGCCTCAGGCAATGTTGCGTTTGTCACCAGTTTCAAATGACGCTGTAAAAGGCGTCATTCTCATATTGTAGATAACGTAGGCTTTGTTc                                                                                                                   <  2:947188/143‑1 (MQ=21)                         gCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCa                                                                                                                  <  1:993036/149‑1 (MQ=255)                                   ccTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATgc                                                                                                        <  1:690066/149‑1 (MQ=255)                                      tCAAATCCAATAAATTGCAGTGAAAGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATgtggc                                                                                                     <  2:934253/149‑1 (MQ=255)                                      gcAAATTCAATAGATGGCAGTGGACGTGTAGGCCTGATAAGGGTAGCGCAGCAGGCAATGTTGCGTTTGTCATCAGTTTCAAAGGTCGCTGTAAAAGGCGTCAGTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATgcggc                                                                                                     <  2:662949/148‑1 (MQ=25)                                            ccAATAAATTGCAGTGAACGAGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTGCATATTGTAGACAACGTAGGCTTTGTTCACGCCGCATCCGGCATGAAc                                                                                               <  1:919597/149‑1 (MQ=21)                                                         gTGAACGTGTAGGCCTGATAAGCGTAGCGCATGATGAAATGTTGCGTGTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCa                                                                                  <  1:503292/149‑1 (MQ=255)                                                         gTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCa                                                                                  <  2:614043/149‑1 (MQ=255)                                                            aaCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCa                                                                                  >  1:559848/1‑146 (MQ=255)                                                            aaCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCa                                                                                  <  2:559848/146‑1 (MQ=255)                                                                 gtAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAAcc                                                                          <  2:859263/149‑1 (MQ=255)                                                                  tAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTATATACAACATAAGCTTTGTTCATGCCGGATTCGGAGTTAACGCCTTATCCGGCATGAAAAccc                                                                         >  1:85686/1‑149 (MQ=21)                                                                     gCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCAGCATGAAAACCCTTc                                                                      >  1:668084/1‑149 (MQ=255)                                                                                gTAGCGCATCAGGCAATGTGGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAg                                                           <  2:364411/149‑1 (MQ=255)                                                                                   gcgcATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTCAAAGGCGTCATTTTCATATTGTAGACCACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACACCTTATCCGGTATGAAAACCCTTCAAATCACATCGAtt                                                        >  1:186912/1‑149 (MQ=18)                                                                                   gcgaATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGAtt                                                        >  2:447707/1‑149 (MQ=255)                                                                                       aTCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGACGCTGTAAAAGGCGTCATTTTACTAGGGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAg                                                    >  1:933271/1‑149 (MQ=17)                                                                                        tCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCAGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGt                                                   >  1:974223/1‑149 (MQ=255)                                                                                          aGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCAAGAAAACCCTTCAAATCCAATAGATTGCAGTGa                                                 >  1:127328/1‑149 (MQ=255)                                                                                           ggCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCCTTTTCATATTGTAGTCAACGTAGGCTTTGTTCATGCCGAATTAGGCGTCAACGACTTAACCAGAATGAAAACCCTTCAAATACAATGGATTGCAGTGaa                                                >  1:157520/1‑149 (MQ=17)                                                                                            gCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTCGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACTCCTTATCCGGCATGAAAACCCTTCAAATCAAATAGCTTTCAATGCAc                                               >  2:226650/1‑149 (MQ=17)                                                                                                tgttgtGTTTGTTTTCAGTTTAAAATGGCGCTGGAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgtgt                                           <  2:1038172/149‑1 (MQ=14)                                                                                                tGTTGGTTTTGTTATAAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTAATGCCGGATGCGGCGTGAACGCCTTATGCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgtgt                                           <  2:207259/149‑1 (MQ=11)                                                                                                  ttGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGAAGCCGGGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAg                                         >  2:897017/1‑149 (MQ=255)                                                                                                    tcttttGTTATCAGTTTCAATTGGCGCTGTAAAAGGCGTCATTTTCATATTGTTGATAATGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGc                                       <  1:306755/146‑1 (MQ=14)                                                                                                     cGTATGTCATCATGTTCAAATGGCGTTGTAAAAGGCGTCATTTTCATATTGTAGACAATGTAGTTTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGcc                                      <  1:79448/149‑1 (MQ=11)                                                                                                           tcatcaGTTTCAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAATGACCGTGTAGACCTGATaa                                >  2:900377/1‑149 (MQ=255)                                                                                                           ccatcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATTTTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGTCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATaa                                <  1:628345/148‑1 (MQ=255)                                                                                                              tcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATc                                                                                       >  2:759657/1‑91 (MQ=255)                                                                                                              tcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATc                                                                                       <  1:759657/91‑1 (MQ=255)                                                                                                                      aaaTGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCAt                     >  2:151438/1‑149 (MQ=32)                                                                                                                       aaTGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAg                                         >  2:910197/1‑128 (MQ=32)                                                                                                                       aaTGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGACTGCAGTGAACGTGTAg                                         <  1:910197/128‑1 (MQ=17)                                                                                                                             gcTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGaaa              >  1:867481/1‑149 (MQ=17)                                                                                                                               tGTAAAAGGCGTCATTTTCATATTGTAGACAACGTATGCTTTGTTCATGCCGGATGCGGCGTGAACCCCTTCTCCGGCATGAAAACCCTTCAACTCCAATAGATTGCAGTGAACGTCGAGCCATCATAAGCCTAGAGCCTCAGGCaaag            >  2:803233/1‑147 (MQ=11)                                                                                                                                       gCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGACATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGttt    >  1:865409/1‑149 (MQ=17)                                                                                                                                         gTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAACGAAGCGCATCAGGCAATGTTGCGATTGt  >  1:359397/1‑149 (MQ=9)                                                                                                                                         gTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCAGCATGAAAACCCTTCAAATCCAATATATTGCAGTGAACATGTAGGCCTGATAAGCGTAGCGCATCAAGCAATGTTGCGTTTGt  >  1:177094/1‑149 (MQ=9)                                                                                                                                         gTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATCCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAAACCTTCAAATCCAATAAATTGCAGTGAACAAGTAGACCTGAAAAGCATAGCGCAACGGGCAATGTTGCGTTTGt  >  1:212968/1‑149 (MQ=11)                                                                                                                                                   |                                                                                                                                           GATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGT  >  NC_000913_3_pae_tpiA/1552075‑1552359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.