Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	275,065	G→A	T18I (ACC→ATC)	insH1 ←	IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	275,065	0	G	A	100.0%	57.4 / NA	24	T18I (ACC→ATC)	insH1	IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/11);  total (13/11)

GACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAA  >  NC_000913_3_pae_tpiA/275005‑275210                                                             |                                                                                                                                                  gACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGTGCTGGTTCGCCCCCTCCGGAg                                                           >  2:604949/1‑149 (MQ=11)   cAAGAAAATCTCTTTTCTTGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGAAAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGc                                                         <  1:305660/149‑1 (MQ=255)    aaGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACCTGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCg                                                        >  1:15459/1‑149 (MQ=25)    aaGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATAATCTCATATCAGGGACTTGTTCGCACATTCCGGAGGCg                                                        >  1:13051/1‑149 (MQ=255)      gAAAATCTGTGTTCAGGGCTCACGCGGCTTAATGCTGAATTCACTGTCGGCGAAGATAAGGTGATGACTCATGATGATCCCGGTTGTATGTCTCCATATTACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGtt                                                      <  2:815299/149‑1 (MQ=11)      gAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGtt                                                      >  1:188927/1‑149 (MQ=37)           tctcTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAg                                                 >  2:808208/1‑149 (MQ=255)           tctcTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAg                                                 >  1:750232/1‑149 (MQ=255)                         tgtcGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTTGTCAATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTTTCTCCTTCCGAAGGCGTTATGAGCTGGCGGCCCtttt                                   >  2:984468/4‑149 (MQ=17)                         tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCGTGATGAACCCTGTTCTATGGCTCCAGATGACAAAAATGAGCTCATATCAGGGACTTGTTCGCACCTTACGGAGGCGTTATAAGCTGGCGGCCCtttt                                   >  1:346560/1‑149 (MQ=17)                         tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGAATTGTCAGCATTGTCCGGAGGCGTTATGAGCTGGCGGCCCtttt                                   <  1:774300/149‑1 (MQ=17)                          gACGGCGCTTACTTGTGAATTCACGGTCGGCGAAGATAAGTTGATGACCCATGCTGAACCCTGTTCTATGGCGCCAGATCACCAACATGATCTCATACCAGGGACTTGTTCGCACCTTCCGGAGGCTTTATGAGCTCGCGGCCCttttt                                  <  2:70402/149‑1 (MQ=17)                           ccGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGGTATGAGCTGGCGGCCCTTTTTg                                 >  1:564921/2‑149 (MQ=255)                            cGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGt                                >  1:131931/1‑149 (MQ=255)                              gctgttACTGCTGAATTCACTGGCGGCGAAGATAAGTTGATGACTTATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTAt                              <  2:374079/145‑1 (MQ=255)                              gcgcTTACTTGTGAATTCACTGTCCGAGAAGATATTTTGGTGTTTAGTCATGAACCCTGTTCTATGGATCCAGGTGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTAt                              <  1:236200/149‑1 (MQ=17)                              gcgcTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGTCTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTAt                              <  1:938509/149‑1 (MQ=255)                                  ttACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGa                          <  1:152888/149‑1 (MQ=255)                                     ctgctgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGatta                       <  1:808208/149‑1 (MQ=255)                                                aCTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCAc            >  1:710188/1‑149 (MQ=255)                                                  tGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCAcca          >  2:745648/1‑148 (MQ=255)                                                       gCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTAt     <  2:188927/149‑1 (MQ=255)                                                         gAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTa   >  1:889659/1‑149 (MQ=255)                                                          aaGATAAGTTGATGACTCATGATGAACCCTGTGCTATGGTTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTaa  <  2:556504/149‑1 (MQ=255)                                                             |                                                                                                                                                  GACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAA  >  NC_000913_3_pae_tpiA/275005‑275210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑