New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 2235628	30 (0.960)	4 (0.130)	4/272	3.9	11.3%	coding (364/1236 nt)	preA	dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
	?	NC_000913_3_pae_tpiA	= 2235642	34 (1.140)					coding (378/1236 nt)	preA	dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTGAAACGGGGTTCCACGCTGCCAAT  >  NC_000913_3_pae_tpiA/2235482‑2235776                                                                                                                                                   |                                                                                                                                                     gtttCAATAATATGTACCAGATTGCTGAACAGCCGTTGCAAGAGAATCTGGCTGCCCTGCGTCGTCTGAAGGAATATTACCCGGACACTGTATTGATGGTTTCGTGCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTg                                                                                                                                                    <  2:160572/147‑1 (MQ=255)                            aaCATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATc                                                                                                                         >  1:46969/1‑149 (MQ=255)                                tCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAgt                                                                                                                     <  2:283157/149‑1 (MQ=255)                                tCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAgt                                                                                                                     <  1:449638/149‑1 (MQ=255)                                   gTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAgtgtc                                                                                                                  >  2:162898/1‑148 (MQ=255)                                          gagaATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTtctc                                                                                                           <  1:280421/149‑1 (MQ=255)                                          gagaATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTtctc                                                                                                           <  1:463578/149‑1 (MQ=255)                                          gagaATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAAGGTAACTtctc                                                                                                           >  1:21487/1‑149 (MQ=255)                                                                   tGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGCGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGCTATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCg                                                                                  >  1:102551/1‑149 (MQ=255)                                                                    gaaggaagATTACCGGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGa                                                                                 >  1:475324/1‑149 (MQ=255)                                                                           gATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAg                                                                          <  1:372302/149‑1 (MQ=255)                                                                           gATTACCCGGACAAAGTATTAATAGCATCGAGCATGAGGAAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGCCCACAAATGACTTCTCATGCGATGGTTat                                                                          >  2:63657/1‑148 (MQ=255)                                                                            aTTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCTCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGc                                                                         <  2:475324/149‑1 (MQ=255)                                                                            aTTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGc                                                                         <  1:255540/149‑1 (MQ=255)                                                                                cccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATg                                                                     >  1:444028/1‑149 (MQ=255)                                                                                    gACAAAGTATTGATCGCTCCGATCATGGGGGAAAATGCGCAGTAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCCAATGACTTCTCATGCGATGGGTAGCGATGTCgg                                                                 <  1:251749/149‑1 (MQ=255)                                                                                         aggaTTGGTCGCTTCGATCAAGGGGGGAAATTAGCAGCAATGGGAGGAGCCGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATTATCGAGTGTAACTTCGCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCaaa                                                            <  2:273611/146‑1 (MQ=255)                                                                                                aTCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCGTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGa                                                     <  1:359620/149‑1 (MQ=255)                                                                                                aTCGCTTCGATCAAGGGCGAAAAAGAGCAGCAACGGGCGATGCTGGCGCGCCGTGTGCAAGAATCTGGCGCGGAGATGAGCGAGTGGAACTTCTCCTGTCCGCAAATGACTTCGCATGCGATGGGTAGCGATGTCGGGCAAAGCCCggc                                                     >  2:325604/1‑148 (MQ=255)                                                                                                     ttCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGa                                                                                                                       <  2:165082/78‑1 (MQ=255)                                                                                                     ttCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGa                                                                                                                       >  1:165082/1‑78 (MQ=255)                                                                                                          tCATGGGGGAAAATGAGCAGCCATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGaa                                           >  2:175433/1‑149 (MQ=255)                                                                                                           cATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGaaa                                          >  1:397196/1‑149 (MQ=255)                                                                                                              gggggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAat                                       <  1:426885/149‑1 (MQ=255)                                                                                                                         ggcaggaATGGGGGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCa                            <  1:32400/148‑1 (MQ=255)                                                                                                                             gcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTgc                        >  1:46519/1‑148 (MQ=255)                                                                                                                                 tGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATg                                                                                    <  1:18794/85‑1 (MQ=255)                                                                                                                                 tGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATg                                                                                    >  2:18794/1‑85 (MQ=255)                                                                                                                                         gCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTGAAACGGGGTTCCa            <  1:155544/149‑1 (MQ=255)                                                                                                                                                   cTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTGAAACGGGGTTCCACGCTGCCAAt  >  1:244176/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTGAAACGGGGTTCCACGCTGCCAAT  >  NC_000913_3_pae_tpiA/2235482‑2235776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.