New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 2530204	34 (1.090)	4 (0.140)	4/268	3.9	10.9%	intergenic (‑28/+43)	ligA/zipA	DNA ligase, NAD(+)‑dependent/FtsZ stabilizer
	?	NC_000913_3_pae_tpiA	= 2530209	33 (1.120)					intergenic (‑33/+38)	ligA/zipA	DNA ligase, NAD(+)‑dependent/FtsZ stabilizer
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTC  >  NC_000913_3_pae_tpiA/2530066‑2530351                                                                                                                                                |                                                                                                                                               ccTGTCGTATTCAGCGTCGAGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCgggg                                                                                                                                           <  1:279950/149‑1 (MQ=255)        tATTCAGCGGCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGaa                                                                                                                                    <  2:544659/149‑1 (MQ=255)              gCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCGCGACAAGCGGGGGTTCGAAgaggag                                                                                                                              >  1:195543/1‑149 (MQ=255)                            cGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCGGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGGGTTAATTTGCCTGaa                                                                                                                >  1:873/1‑149 (MQ=255)                                          caTGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATGTCGCACCATCAATGCTAAAAAACCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGtt                                                                                                  >  2:169058/1‑149 (MQ=255)                                           aTGGTAACGACACTCACGATCGCGACGCGTCTATCGCAGTTCTGGCACTGGTTGTTCTATTGATTACTTACCGGAGCATACCTGAAAGCAACCGCGGTCTTGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTg                                                                                                 <  1:268523/149‑1 (MQ=255)                                                aaaGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTc                                                                                            <  1:452083/149‑1 (MQ=255)                                                    atatTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTg                                                                                        <  2:35225/149‑1 (MQ=255)                                                     tatTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGa                                                                                       <  2:22232/149‑1 (MQ=255)                                                     tatTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGa                                                                                       <  1:114089/149‑1 (MQ=255)                                                           tgatgGCGAAGCTGCGTTCGCAGTTCTGTCAGTTCTTGTTCGAATGATTCCATATCGCACCATCAATGCTAAATACCCCCGACGAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTcgc                                                                                 <  2:324158/149‑1 (MQ=255)                                                                  gAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAAGCGTTGGCGTCTTTGACTTCGCGgatgat                                                                          >  1:120651/1‑149 (MQ=255)                                                                     gCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTc                                                                       <  1:337632/149‑1 (MQ=255)                                                                        tcgtTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTg                                                                    <  2:352566/149‑1 (MQ=255)                                                                        tcgtTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTg                                                                    <  1:304498/149‑1 (MQ=255)                                                                        tcgtTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTg                                                                    >  1:409436/1‑149 (MQ=255)                                                                                         aGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCt                                                   >  1:39736/1‑149 (MQ=255)                                                                                         aGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCt                                                   <  2:489527/149‑1 (MQ=255)                                                                                         aGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGGTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTCCTCGCGCACTTTCt                                                   >  1:4657/1‑149 (MQ=255)                                                                                           ttgttgttCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGc                                                 <  2:39736/149‑1 (MQ=255)                                                                                            tgttgttCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCg                                                <  2:286090/149‑1 (MQ=255)                                                                                              ttgttCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGa                                              <  2:402945/149‑1 (MQ=255)                                                                                              ttgttCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGa                                              >  1:547277/1‑149 (MQ=255)                                                                                                          ttCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACgg                                  >  1:145509/1‑149 (MQ=255)                                                                                                               tatCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGAAGTCATCATACGGCGCTg                             >  1:211844/1‑149 (MQ=255)                                                                                                                    cACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTg                                                                                        >  2:398719/1‑85 (MQ=255)                                                                                                                    cACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTg                                                                                        <  1:398719/85‑1 (MQ=255)                                                                                                                       ctcacttGCTAAAATCCCCGCACAATCGGGGGTTCGAAGAGGATTTAATTTTCCTTAAGTGTATCAGGCGTTGGCGGCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCTATTTCTGCTGAGTCATCATACGGCGCTGATCGTCaa                     <  2:328164/143‑1 (MQ=255)                                                                                                                       cATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAAttt                                                                                                                       >  2:169845/1‑51 (MQ=255)                                                                                                                       cATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAAttt                                                                                                                       <  1:169845/51‑1 (MQ=255)                                                                                                                          cAATGCTAAAAACCCCCGACAAGTGGCGGGGTGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCTCTGATCGTCAAGCa                  <  2:253404/149‑1 (MQ=255)                                                                                                                               cTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCCGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGaca             <  1:158261/149‑1 (MQ=255)                                                                                                                                         ccGACAGGGGGGGGTTCGAAGAGGAGTTAATTCGCCTTAAGTGTATCAGGCTTTGGCGTCTTTGACTTCGCGGATGACGTCCTGGTACTCGCGCAATTTCTGCGCAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACtt   <  2:20133/149‑1 (MQ=255)                                                                                                                                          cGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTc  >  2:45396/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                               CCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTC  >  NC_000913_3_pae_tpiA/2530066‑2530351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.