Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1978503–1979269	1979278	10–776	24 [0]	[0] 24	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1978502	0 (0.000)	20 (0.680)	18/268	0.7	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_pae_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

TAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGC  >  NC_000913_3_pae_tpiA/1978378‑1978644                                                                                                                             |                                                                                                                                               tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggg                                                                                                                        >  1:284700‑M1/1‑125 (MQ=255)            cTAAATCGACGCAACTTTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                             <  1:335805‑M1/149‑36 (MQ=255)             tAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatc                                                                                                            >  1:87370‑M1/1‑113 (MQ=255)                   gACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTAGTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaa                                                                                                      >  1:474386‑M1/1‑107 (MQ=255)                                  gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatac                                                                                                                                    >  1:230424‑M1/1‑92 (MQ=255)                                  gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatac                                                                                                                                    <  2:230424‑M1/104‑13 (MQ=255)                                  gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGgataacagctacattggttatgggtgtctttcaatctgttaaaaaggttttcgctac                                                                                       >  1:264349‑M1/1‑92 (MQ=255)                                       tACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctag                                                                                  >  1:135014‑M1/1‑87 (MQ=255)                                          acGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgcttcgctagcaa                                                                               >  2:293811‑M1/1‑84 (MQ=255)                                                      cGGAGGCGCGCTGCGAGTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                                   <  2:314722‑M1/149‑78 (MQ=255)                                                         aGGGGGGCTGCGATTTTCACTAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacag                                                                <  1:340403‑M1/149‑81 (MQ=255)                                                                    gATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaa                                                     >  1:205287‑M1/1‑58 (MQ=255)                                                                      attgcaataATGCGTTATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtatcagtcatacttttgttttgggtgtatttcaatctgttaaacagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaat                                                   <  2:82643‑M1/145‑94 (MQ=255)                                                                           aataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgatagaaagcaaacatgaaacaggaataatctaaatgggat                                              >  2:329322‑M1/1‑51 (MQ=255)                                                                                     gATTCAGATCAAAAAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcaca                                    <  2:474386‑M1/149‑109 (MQ=255)                                                                                                   aaaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactca                      >  1:60768‑M1/1‑27 (MQ=255)                                                                                                   aaaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttaggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatggtatgtttcgcacagtcaaaataactca                      >  2:289619‑M1/1‑27 (MQ=255)                                                                                                             aTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataa            >  1:386646‑M1/1‑17 (MQ=255)                                                                                                                  ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtgaaaaggattttgtcgatgctagcaaccaaaaatgaaacaggaataatcgaaatgggatgttcagcacactcaaaataacacagcgtaaataagcatc       >  2:201294‑M1/1‑12 (MQ=255)                                                                                                                    aaCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggctgttactcgcagtcaaaataactcaccgtaaataatcatctg     <  2:369424‑M1/149‑140 (MQ=255)                                                                                                                      cATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgct   <  1:380874‑M1/149‑142 (MQ=255)                                                                                                                       aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgcgacgctagcaagcaaaaatgaaacaagaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgcta  <  2:126032‑M1/149‑143 (MQ=255)                                                                                                                        tAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                                   <  2:282924‑M1/83‑78 (MQ=255)                                                                                                                        tAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                                   >  1:282924‑M1/1‑6 (MQ=255)                                                                                                                             |                                                                                                                                               TAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGC  >  NC_000913_3_pae_tpiA/1978378‑1978644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑