Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 257908–258674 258683 10–776 25 [0] [0] 25 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 2579070 (0.000)24 (0.810) 22/268 0.4 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913_3_pae_tpiA 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

ATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTT  >  NC_000913_3_pae_tpiA/258540‑258831
                                                                                                                                                |                                                                                                                                                   
tgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGc                                                                                                                                                 >  1:410106‑M2/145‑149 (MQ=255)
                 aaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCtt                                                                                                                                >  2:18366‑M2/128‑149 (MQ=255)
                         ccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtgcccaaccaaagtgcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                                                                        <  1:128417‑M2/30‑1 (MQ=255)
                                   aaaaaagttgcatgacaacaggcgatagcactgacgttaccgagtggacacccgaagagcagattgagcaaaaaatttaccgccctaggcccgtatatttgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGt                                                                                                              <  2:226845‑M2/40‑1 (MQ=255)
                                               tcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactcggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTGTGGCTGTTTGAGTCACCGt                                                                                                  >  2:376531‑M2/98‑149 (MQ=255)
                                                 acaacaggagatagcaatgacggtaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGa                                                                                                <  1:280539‑M2/54‑1 (MQ=255)
                                                            tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCg                                                                                     <  1:454181‑M2/65‑1 (MQ=255)
                                                            tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCAgcccca                                                                                     <  1:282965‑M2/65‑2 (MQ=255)
                                                                aatgacgctacagagtggacacccgaagagcagattgatcacaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                                                                 <  2:346322‑M2/69‑1 (MQ=255)
                                                                  tgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccccactaggcccgtattttcgtgaaggtaAATGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTg                                                                               >  1:422508‑M2/79‑149 (MQ=255)
                                                                     cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                            <  2:427561‑M2/74‑1 (MQ=255)
                                                                      gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTg                                                                           <  1:4152‑M2/75‑1 (MQ=255)
                                                                           cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                                                                      >  1:485274‑M2/70‑149 (MQ=255)
                                                                                         aagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggat                                                        <  1:18366‑M2/94‑1 (MQ=255)
                                                                                          agagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatg                                                       <  1:38738‑M2/95‑1 (MQ=255)
                                                                                             gcagattgatcaaaaaatttaccgccctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAg                                                    <  1:156960‑M2/98‑1 (MQ=255)
                                                                                                        aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTTGTGGATGGAGCTGGAAGCGCa                                         <  2:485274‑M2/109‑1 (MQ=255)
                                                                                                         aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAg                                        >  2:467388‑M2/40‑149 (MQ=255)
                                                                                                         aaaaatttaccgcactaggcccctatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGAGCGGGGATTTTGGGGCGCGTGGGAGGAGCtggaagtccag                                        >  2:161666‑M2/40‑144 (MQ=255)
                                                                                                                   cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTTTTTTTTTTTTCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGTTGGCGCTGGAAGCGCAGGGATCCCGtt                              >  2:431788‑M2/30‑149 (MQ=255)
                                                                                                                   cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAAACGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtt                              <  1:152004‑M2/120‑1 (MQ=255)
                                                                                                                      actaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGGGAATTCTGGGGCAGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTa                           >  2:433665‑M2/27‑149 (MQ=255)
                                                                                                                           gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGATGAGGGGGGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTAc                      >  1:13668‑M2/22‑149 (MQ=255)
                                                                                                                                       gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGttt          >  1:339205‑M2/10‑149 (MQ=255)
                                                                                                                                               aAGTGCAAAGATAAGCGATTCATTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGtt  <  2:13293‑M2/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTT  >  NC_000913_3_pae_tpiA/258540‑258831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: