Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1299499–1300695 1300697 3–1199 28 [0] [0] 28 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 12994980 (0.000)26 (0.860) 24/276 0.3 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAAT  >  NC_000913_3_pae_tpiA/1300550‑1300844
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ttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtggaggaaagtgctaaa                                                                                                                                                    >  1:77560‑M2/149‑149 (MQ=255)
 tctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctgaat                                                                                                                                                   >  1:99240‑M2/148‑149 (MQ=255)
    caaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataat                                                                                                                                                <  1:295512‑M2/5‑1 (MQ=255)
     aaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccacggcccttggtaaaggactgtgctaaataata                                                                                                                                               >  1:3125‑M2/144‑149 (MQ=255)
        ttacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATc                                                                                                                                            <  2:240912‑M2/9‑1 (MQ=255)
                aggtataaggatttccttaactgcttggcctcaccatcatgttagtttcgccacatcataatcctgggcttgctgaagagtaattgacatgatattattaattccactgcctttggtagaggatagtgctaaATAATAATCAATTGTTa                                                                                                                                    <  1:417802‑M2/17‑1 (MQ=255)
                  ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgcgaaATAATAATCAATTGTTaaa                                                                                                                                  >  1:426457‑M2/131‑149 (MQ=255)
                  ttataaggatttccataactgcttctcttcactatcatgttcatttcaatccaacataatcctgggcttgctgaataataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                                  <  1:125711‑M2/19‑1 (MQ=255)
                          atttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgtg                                                                                                                          <  2:317133‑M2/27‑1 (MQ=255)
                              ccttaactgcttctcctcaccatcatgttattttagccacagcagaatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAtt                                                                                                                      >  1:270538‑M2/119‑149 (MQ=255)
                                           tcctcaccctcatggtattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATCATCAATTGTTTAATTATTGTGCATTTCACTACTGtaat                                                                                                         >  2:511273‑M2/106‑148 (MQ=255)
                                                accatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTAACTACTGGAACTGTaa                                                                                                    >  2:339364‑M2/101‑149 (MQ=255)
                                                      ttgtaattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaactgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaa                                                                                              >  2:417932‑M2/95‑149 (MQ=255)
                                                        gttattttcgccacatgataatcctgggcttactgaagaataattgaaatgatattattaattccactgcatttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATGGTGCATTTCAGTACCGGAactgtaataaacaca                                                                                            >  2:193798‑M2/93‑142 (MQ=255)
                                                           attttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                         <  2:239405‑M2/60‑1 (MQ=255)
                                                                                    ctggcttaagaataattgaaaggagattattaattccactgcctttggtagaggaaagtgctaaATAATAATTAATTGTTAAATTATTGTGAATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAttt                                                                <  2:115647‑M2/85‑1 (MQ=255)
                                                                                      tgctgaacaattattgaaatgatattattaattccacagcctttggtataggaaagtgctaaATAATAATCAAGTGTTAAATCATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttga                                                              <  2:191451‑M2/87‑1 (MQ=255)
                                                                                        gtgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                            <  1:286874‑M2/89‑1 (MQ=255)
                                                                                          gaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgattga                                                          <  1:233498‑M2/91‑1 (MQ=255)
                                                                                                  attgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAg                                                  <  1:277686‑M2/99‑1 (MQ=255)
                                                                                                   ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGa                                                 >  2:317195‑M2/50‑149 (MQ=255)
                                                                                                    tgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGa                                                                                          <  2:279287‑M2/59‑1 (MQ=255)
                                                                                                    tgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGa                                                                                          >  1:279287‑M2/49‑107 (MQ=255)
                                                                                                    ggaaatgaaattattaagtccaaggcctttggtagaggaaagcgctaaATAATAATCAATTGTTAAATTATTGTGCATTCGACTACTGGAACTGTAATGAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAt                                                <  2:3125‑M2/101‑1 (MQ=255)
                                                                                                                attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGc                                    >  2:176535‑M2/37‑149 (MQ=255)
                                                                                                                                    tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAtt                >  2:67015‑M2/17‑149 (MQ=255)
                                                                                                                                                 taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAACACAGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCaa   <  1:406513‑M2/146‑1 (MQ=255)
                                                                                                                                                  aaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGAGGTAATAAAAACTGGGGTTTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAAt  <  1:406333‑M2/147‑1 (MQ=255)
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ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAAT  >  NC_000913_3_pae_tpiA/1300550‑1300844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: