Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,110,747 G→T intergenic (+62/+784) cdh → / ← yiiQ CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,110,7470GT98.7% 272.8 / NA 79intergenic (+62/+784)cdh/yiiQCDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (46/32);  minor base C (0/1);  total (46/33)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCA  >  NC_000913_3_pae_tpiA/4110601‑4110890
                                                                                                                                                  |                                                                                                                                               
gCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAggctgc                                                                                                                                               <  2:310031/149‑1 (MQ=255)
     cGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCccgc                                                                                                                                          <  1:454398/149‑1 (MQ=255)
         acaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGAAAAGTAAGTGCCGGAAATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCcgcg                                                                                                                                      >  1:164632/1‑149 (MQ=255)
           aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAg                                                                                                                                    >  2:549279/1‑149 (MQ=255)
           aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAg                                                                                                                                    >  1:449168/1‑149 (MQ=255)
            aCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGc                                                                                                                                   >  2:514413/1‑149 (MQ=255)
                aaTCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGCGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGtt                                                                                                                               >  1:78594/1‑149 (MQ=255)
                    tACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGc                                                                                                                           <  2:449168/149‑1 (MQ=255)
                       tGACGCTTAACCGTGCTGCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGcaac                                                                                                                        <  2:146794/149‑4 (MQ=255)
                             ttAACCGTGCTTCAGCCGAGGAAATTCAGCATCATCAGTGTGGGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAGGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTaa                                                                                                                  <  1:112431/149‑1 (MQ=255)
                               aaCCGGGCTTCCGCCGAGGAAATTCAGGATAATCAGTGTGAGAGTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGa                                                                                                                <  1:548140/149‑1 (MQ=255)
                                 ccGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGCCTTAAGGCTGCCCCGCCGCGAGCGGTTTCGGCAATTCTAAGagc                                                                                                              >  1:117374/1‑147 (MQ=255)
                                 ccGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATc                                                                                                              >  1:311115/1‑149 (MQ=255)
                                   gTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCtt                                                                                                            <  1:304350/149‑1 (MQ=255)
                                     gCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTaa                                                                                                          <  1:321814/149‑1 (MQ=255)
                                     gCTACAGCCGAGGAAATGCAGGATCATCAGTGTGAGATTTCGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTaa                                                                                                          <  1:455785/149‑1 (MQ=255)
                                       ttCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGa                                                                                                        >  1:25645/1‑149 (MQ=255)
                                         cAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGata                                                                                                      <  1:9100/149‑1 (MQ=255)
                                            ccGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTCGGGAGAAGATTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATgg                                                                                                   <  1:290299/149‑1 (MQ=255)
                                             cGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGGGCGGTTTCGCCAATTCTAAGATCTTTAGCTATggg                                                                                                  >  2:46115/1‑149 (MQ=255)
                                              gAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGt                                                                                                 >  1:547523/1‑149 (MQ=255)
                                              gAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGAAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGt                                                                                                 >  1:362947/1‑149 (MQ=255)
                                               aGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTc                                                                                                >  2:90066/1‑149 (MQ=255)
                                                 gAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCtt                                                                                              >  1:412953/1‑149 (MQ=255)
                                                     ttCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGc                                                                                          >  2:491952/1‑149 (MQ=255)
                                                      tCAGGATCATCAGTGGGAGATTTTGCTTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGcc                                                                                         <  2:241236/149‑1 (MQ=255)
                                                          gATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCa                                                                                     >  1:170073/1‑149 (MQ=255)
                                                              atcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCAGGCAACTATCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAAGTCTAAGATCTTAGAATATGGGTCTTTTGGCTTCACGGc                                                                                 >  2:295812/1‑149 (MQ=255)
                                                               gcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCt                                                                                <  1:28164/148‑1 (MQ=255)
                                                                     tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAg                                                                                                                                    >  1:102705/1‑91 (MQ=255)
                                                                     tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAg                                                                                                                                    <  2:102705/91‑1 (MQ=255)
                                                                     tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCtt                                                                          >  1:161080/1‑149 (MQ=255)
                                                                     tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAAGCCGGCCCCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCtt                                                                          <  1:41952/149‑1 (MQ=255)
                                                                         aTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTACCTCCCCAAGGAAGGGGTCTTTTGCCTTCACGGCTGCGCTTGCCa                                                                      >  1:514042/1‑149 (MQ=255)
                                                                         aTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGCGTCTTTTGCCTTCACGGCTGCGCTTGCCa                                                                      >  1:471931/1‑149 (MQ=255)
                                                                                gTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGAATTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAacac                                                               >  1:216418/1‑149 (MQ=255)
                                                                                 ttAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCaa                                                                  >  2:154026/1‑145 (MQ=255)
                                                                                 ttAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCaa                                                                  <  1:154026/145‑1 (MQ=255)
                                                                                 ttAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAGCACt                                                              >  1:164649/1‑149 (MQ=255)
                                                                                 ttAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACt                                                              >  1:333408/1‑149 (MQ=255)
                                                                                 ttAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACt                                                              >  1:329025/1‑149 (MQ=255)
                                                                                 ttAAGGCGAAGAGTTAAGGAAAGTAAGTGACGGTCCTCAAAGCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACt                                                              >  1:408406/1‑149 (MQ=255)
                                                                                    aGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTccc                                                           >  1:159067/1‑149 (MQ=255)
                                                                                    aGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTccc                                                           <  2:490619/149‑1 (MQ=255)
                                                                                    aGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTccc                                                           <  2:262537/149‑1 (MQ=255)
                                                                                     ggCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTcccc                                                          >  2:361449/1‑149 (MQ=255)
                                                                                         aaGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTCAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGt                                                      <  1:361449/149‑1 (MQ=255)
                                                                                           gagTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGc                                                    <  1:549279/149‑1 (MQ=255)
                                                                                            agTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGcc                                                   <  2:316859/149‑1 (MQ=255)
                                                                                              ttAAGGAAAGTAAGTGCCGTATATGAAATCCGGCACCTGTCAGACTAAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGCTCGTAAGATATGGGTCTTTTGCCTTCACGGGTGCGCTTGCCACCCACACTCCCCTCGTGCCGa                                                 >  2:77156/1‑149 (MQ=255)
                                                                                              ttAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCGGCAACACTCCCCTCGTGCCGa                                                 >  1:72129/1‑149 (MQ=255)
                                                                                              ttAAGGAAAGGAAGAGCCGGAAATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGa                                                 <  1:167508/149‑1 (MQ=255)
                                                                                               tAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAACGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTTACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAg                                                >  1:477646/1‑149 (MQ=255)
                                                                                                 aGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGCTCTTCGGTGAGGGGGCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGtt                                              >  1:266472/1‑149 (MQ=255)
                                                                                                  ggAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGttt                                             >  1:110840/1‑149 (MQ=255)
                                                                                                   gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGGGCCGAGTTTa                                            >  1:263225/1‑149 (MQ=255)
                                                                                                        taagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAgcgc                                       >  1:171072/1‑149 (MQ=255)
                                                                                                         aagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCATGCCGCGTTTAAGcccg                                      >  2:421577/1‑149 (MQ=255)
                                                                                                            tGCCGGATATGAATTCCGGCACCTGTCGGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCt                                   <  1:437484/149‑1 (MQ=255)
                                                                                                            tGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTACGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCCGCCACACCCCCCTCGTGCCCAGGTTAAGCCCGGCt                                   >  1:523304/1‑149 (MQ=255)
                                                                                                              ccGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGCGCTTAAACTCGGcacg                                 >  1:209300/1‑146 (MQ=255)
                                                                                                                 gttatGAAATCCGGCACCTGTCAGATTTAAGGTCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                              <  1:426791/147‑1 (MQ=255)
                                                                                                                    atGAAATCCGGCACGTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTc                           <  1:29861/149‑1 (MQ=255)
                                                                                                                    atGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTc                           <  2:547523/149‑1 (MQ=255)
                                                                                                                     tGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTcc                          >  1:502125/1‑149 (MQ=255)
                                                                                                                      gAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCct                         >  2:221413/1‑149 (MQ=255)
                                                                                                                       aaaTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGGGCCGAGTTTAAGCGCGGCTTCAACGTCctc                        >  1:92527/1‑149 (MQ=255)
                                                                                                                        aaTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACATTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctct                       <  1:491952/149‑1 (MQ=255)
                                                                                                                        aaTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctct                       >  1:409669/1‑149 (MQ=255)
                                                                                                                         aTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCtttc                      >  1:321586/1‑149 (MQ=255)
                                                                                                                             ggCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGct                  <  1:341708/149‑1 (MQ=255)
                                                                                                                              gCACCTGTCAGACTTAAGGCTGCCCCGCCGCGGGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctc                 <  1:135144/149‑1 (MQ=255)
                                                                                                                              gCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctc                 >  1:525731/1‑149 (MQ=255)
                                                                                                                               cACCTGTAAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACCCTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctct                <  1:315514/149‑1 (MQ=255)
                                                                                                                                    gTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATa           <  1:229012/149‑1 (MQ=255)
                                                                                                                                    gTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATa           <  1:176674/149‑1 (MQ=255)
                                                                                                                                     tCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGATTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAc          >  2:15433/1‑149 (MQ=255)
                                                                                                                                       aGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGt                                                                                                 >  2:418496/1‑60 (MQ=255)
                                                                                                                                       aGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGt                                                                                                 <  1:418496/60‑1 (MQ=255)
                                                                                                                                             aaGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCa  <  1:77156/149‑1 (MQ=255)
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GCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCA  >  NC_000913_3_pae_tpiA/4110601‑4110890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑