New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	3123452 =	37 (0.940)	5 (0.130)	5/270	4.7	11.9%	coding (354/2172 nt)	glcB	malate synthase G
	?	NC_000913_3_pae_tpiA	3123480 =	39 (1.050)					coding (326/2172 nt)	glcB	malate synthase G
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCT  >  NC_000913_3_pae_tpiA/3123309‑3123598                                                                                                                                                |                                                                                                                                                    aCCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAg                                                                                                                                                <  2:582356/149‑1 (MQ=255)       tCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTTCCGTATAAAGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCgg                                                                                                                                          >  1:421909/1‑149 (MQ=255)         aCCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGcc                                                                                                                                        >  1:661017/1‑149 (MQ=255)                    gATCGGAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCctggctg                                                                                                                             <  2:598896/149‑1 (MQ=255)                      tCGGAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGt                                                                                                                           <  1:351705/149‑1 (MQ=255)                             cGCTGACCATCACCCCTTCCTGCGGGATGATGTCGCTGCCGTATCACGCATCGTACAGTGTGCCCGAGCGAGCGTTCGCCGCGTTCAGCGCGTGGCGGGCGTTCATTGCCGGAATCACCAGCTGCGGCGCCGCCTGGCTCGTGATTTCg                                                                                                                    <  2:548955/149‑1 (MQ=255)                                tGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGTTGATTTCGCTg                                                                                                                 <  1:436993/149‑1 (MQ=255)                                         ccccTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGccc                                                                                                        <  2:561938/149‑1 (MQ=255)                                            cTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCCGTCTGGTGATTTCGCTGTCCATGCCCGTg                                                                                                     >  2:319546/1‑149 (MQ=255)                                            cTTCCTGCGGGATGATGGCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTg                                                                                                     <  2:379928/149‑1 (MQ=255)                                             ttCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGAGAGCCACAGCGAGAGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGACCCACCAGCTGCGGCCCCGCCTGGCCGGTGCTTTCGCCGTCAATGCCCgggg                                                                                                    >  2:298271/1‑149 (MQ=255)                                                     ggATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGccc                                                                                                        >  2:600631/1‑137 (MQ=255)                                                     ggATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGccc                                                                                                        <  1:600631/137‑1 (MQ=255)                                                          atgTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CAc                                                                                      <  1:409500/149‑1 (MQ=255)                                                               gCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGCGCGTTCCCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGGTTCCACCGT‑CAAGCGCt                                                                                 >  1:298402/1‑149 (MQ=255)                                                                cTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTc                                                                                >  2:444087/1‑149 (MQ=255)                                                                 tGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTcc                                                                               >  1:245272/1‑149 (MQ=255)                                                                           cGCATCGTACCGTGTGCCCCAGCGAGCGTTCGCCGCGTTCCGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCCGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGTTTGcttca                                                                     <  2:84554/149‑5 (MQ=255)                                                                           cGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCa                                                                     >  1:258150/1‑149 (MQ=255)                                                                                 gTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCCCCGGTTGCGGCACCAGGt                                                               >  2:240939/1‑149 (MQ=255)                                                                                       tGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATGTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCa                                                         <  2:502361/149‑1 (MQ=255)                                                                                             ccAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCAc                                                   >  1:630937/1‑149 (MQ=255)                                                                                                  gagcgTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGG‑CACGACCTTACACTCTCTCCGGTTGCGGCACCAGGAAGCCCAGTTCACGCAgg                                              >  1:402125/1‑149 (MQ=255)                                                                                                      gTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGGGCTCCGGGTGCGGCCCCCGGTGGCCCACTTCACGCAGGAAAg                                          >  2:285902/1‑149 (MQ=255)                                                                                                          gccgcGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCACGAAAGAttt                                      >  1:103804/1‑149 (MQ=255)                                                                                                           ccgcGTTCAGCGCGTAGCGGGCGGTCATTGCCGGGACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCGCCGT‑CACGCGCCCCGGTTGCGGCACCAGGTAGCCAAGTTCACGCAGGCAAGAtttg                                     >  2:368237/1‑148 (MQ=255)                                                                                                              cgTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAg                                  >  1:403289/1‑149 (MQ=255)                                                                                                               gTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAgg                                 <  2:658812/149‑1 (MQ=255)                                                                                                               gTTCAGCGCGTAGCGGGCGTTAATTGCCGGAACCACCAGCTGCGGCCACGACTGGGTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCCACAGGTAGCACAGTTCACGCGGGAAAGGTTtatgag                                 >  2:221535/1‑146 (MQ=255)                                                                                                               gTTCAGCGCGGAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCAACAGGTAGCCCAGTTCACGCAGGAAAGATTTATAgg                                 <  2:238759/149‑1 (MQ=255)                                                                                                                             gggCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCtttt                   <  1:375298/149‑1 (MQ=255)                                                                                                                               gCGTTCCTTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTAc                 <  1:436750/149‑1 (MQ=255)                                                                                                                                 gTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCg               <  1:473977/149‑1 (MQ=255)                                                                                                                                        gCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATCGGCCGCTTTATCTTTTACCGGCCCCgg        >  2:159359/1‑149 (MQ=255)                                                                                                                                         ccGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTtat                                    >  2:429449/1‑120 (MQ=255)                                                                                                                                         ccGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTtat                                    <  1:429449/120‑1 (MQ=255)                                                                                                                                              accaccAGCTGCGGCCCCGCCTGGCCGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATtgct  <  1:127959/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                    ACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCT  >  NC_000913_3_pae_tpiA/3123309‑3123598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.