Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 264,098 G→T L50L (CTC→CTA ykfH ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA264,0980GT100.0% 114.9 / NA 39L50L (CTC→CTAykfHuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/18);  total (21/18)

GCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGAT  >  NC_000913_3_pae_tpiA/263955‑264238
                                                                                                                                               |                                                                                                                                            
gCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCGCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc                                                                                                                                         <  1:210704/149‑1 (MQ=255)
    ccAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCg                                                                                                                                     >  1:623728/1‑149 (MQ=255)
        gcgcAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGa                                                                                                                                 >  1:206237/1‑149 (MQ=255)
                 aaTCTCCCGGTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGaa                                                                                                                        <  1:601209/149‑1 (MQ=255)
                                   ccTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAAt                                                                                                      >  2:211809/1‑149 (MQ=255)
                                    cTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGCTGTACTg                                                                                                     >  2:252962/1‑149 (MQ=255)
                                     tGGGAGTGTTGCTTGTTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTTGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGa                                                                                                    <  2:364416/149‑1 (MQ=25)
                                      ggTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGAc                                                                                                   <  1:211809/149‑1 (MQ=255)
                                       gTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACa                                                                                                  >  1:475039/1‑149 (MQ=255)
                                             ttGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCt                                                                                            >  1:11211/1‑149 (MQ=255)
                                                  cATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGt                                                                                       <  2:575203/149‑1 (MQ=255)
                                                   aTTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTg                                                                                      >  1:313318/1‑149 (MQ=255)
                                                     tACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCATATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGcc                                                                                    >  2:542797/1‑149 (MQ=255)
                                                       cAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCAc                                                                                  <  2:313318/149‑1 (MQ=255)
                                                          ttCTCCACTATTGGCAGTTGAGGGTGGTGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGATCGGCCTCAGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTgg                                                                               <  2:104579/149‑1 (MQ=21)
                                                          ttCTCCACTATGGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGGGCCACTgg                                                                               >  1:427883/1‑149 (MQ=255)
                                                           tctcCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGt                                                                              <  2:206237/149‑1 (MQ=255)
                                                              ccACTAGTGTCGGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTAGACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTCATTACAGACGCTACCGTGCCACTGGTAtt                                                                           <  2:427883/149‑1 (MQ=255)
                                                               cACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAttt                                                                          >  2:407837/1‑149 (MQ=255)
                                                                  tATTGTCAGTTCAGGGTGATGCTCATCAGACAGGTATATGGCCCTTTGCGATCCAGGGGGGTTTCGGCGTATACCGCTAGGACTCCAGCGATATCCGGAACGTGCCTGCCGGTGTAATGACAGCCGCTACCGTGCCACTGGTCTGTGcc                                                                       >  1:586440/1‑149 (MQ=18)
                                                                     tGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGt                                                                    >  2:439324/1‑149 (MQ=255)
                                                                           ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTa                                                              <  1:541318/149‑1 (MQ=255)
                                                                           ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTa                                                              <  1:403642/149‑1 (MQ=255)
                                                                                      gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTc                                                   <  1:439324/149‑1 (MQ=255)
                                                                                        tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCgg                                                 <  2:659006/149‑1 (MQ=255)
                                                                                        tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCgg                                                 >  2:558120/1‑149 (MQ=255)
                                                                                          atcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGa                                               >  1:581544/1‑149 (MQ=255)
                                                                                              ggcaggTATAGGGCCCATGGCGGTCCTGGCGGCGTTAGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGGGTAATGACAGACGCTACCTGGCCACTGGTACTTGCCGGGCCAGTAGCGAAAGATGCGGGACTCa                                           >  2:375064/1‑149 (MQ=255)
                                                                                                 aggTATAGGGCTCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGa                                        <  1:52664/149‑1 (MQ=255)
                                                                                                   gTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCGGTAGCGAAAGATTCGGTACTCAGGATg                                      >  1:588322/1‑149 (MQ=255)
                                                                                                          gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCgg                               <  1:10953/149‑1 (MQ=255)
                                                                                                            ccATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTGCCGTGCCACTGGTATTTGCCGGTGAAGTAGCGAACGATTCGGGGCTCAAGCTGCTGACGGta                             >  2:333075/1‑149 (MQ=17)
                                                                                                               ttGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTACGGCGATATCCGGAACGTACCTGCCGGTGTAATAACAGACGCTACCGTCCCACTGGTATTTGCCAGGGAACTAGCACAAGCTTGGCAACTCCAGATGCTGTGGGGatat                          >  2:368671/1‑149 (MQ=255)
                                                                                                                    gTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCa                     <  1:690911/149‑1 (MQ=255)
                                                                                                                     tgcTGGCGGCGTGCGGCGTATACCGCTCGGGATCCGGGGTTATTCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCAt                    <  1:16492/147‑1 (MQ=14)
                                                                                                                       gcggcggcGTGCGGCGTAGACCGCTAGGGCGCCGGCGAGATCCGGGACGTCGGTGGCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGAGTCAGGATGCTGGCGGTATATCGTCATTg                  <  2:279712/147‑1 (MQ=14)
                                                                                                                             gcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGc            >  1:178771/1‑149 (MQ=255)
                                                                                                                                   ggCGTATAACGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTACCGGTGCAGTAGCGAAAGATTAGGGAAGAAAGATTCTGGCGGGATATAGTCATTGCACTGCGTTTAc      >  2:36645/1‑149 (MQ=14)
                                                                                                                                       tataCCGCTAGGAGTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGCCAGACGCTACGGTGCCACTGGTGTTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCCTTACCGTGCGTTTACTggt  >  1:568246/1‑147 (MQ=17)
                                                                                                                                               |                                                                                                                                            
GCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGAT  >  NC_000913_3_pae_tpiA/263955‑264238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: