New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 191476935 (1.200)4 (0.150) 4/262 3.5 11.0% coding (48/2049 nt) prc carboxy‑terminal protease for penicillin‑binding protein 3
?NC_000913_3_pae_tpiA = 1914775 32 (1.180)coding (42/2049 nt) prc carboxy‑terminal protease for penicillin‑binding protein 3
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGATCGAGGTCGAACTGGCGATAATGAGAACGGGTGAAGCGCGACGTTACGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATT  >  NC_000913_3_pae_tpiA/1914626‑1914914
                                                                                                                                               |                                                                                                                                                 
tGATCGAGGTCGAACTGGCGATAATGAGAACGGGTGAAGCGCGACGTTACGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGc                                                                                                                                              >  2:499162/1‑149 (MQ=255)
  aTCGAGGTCCAAGGGGCGATAATGAGAACGGGGGAAGCGCGACGTTACGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCaa                                                                                                                                            <  2:144559/149‑1 (MQ=255)
      aGGTCGAACTGGCGATAATGAGAACGGGTGAAGCGCGACGTTACGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTACTACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAg                                                                                                                                        <  1:110348/149‑1 (MQ=255)
            aaCTGGCGATAATGAGAACGGGTGAAGCGCGACGTTACGCGCTCACTTACCGTCGCATGCTGCGTATCTTCCTTTACTACCGGAATTTCATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGc                                                                                                                                  >  1:380761/1‑149 (MQ=255)
               tGGCGATAATGAGAACGGGTGAAGCGCGACGTTACGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTaa                                                                                                                               <  2:1005/149‑1 (MQ=255)
                      aaTGAGAACGGGTGAAGCGCGACGTTACGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTa                                                                                                                        >  2:476961/1‑149 (MQ=255)
                       aTGAGAACGGGTGAAGCGCGACGTTACGCGGAAAAACCGCGTCTGATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTaa                                                                                                                       >  1:124756/1‑149 (MQ=255)
                         gagaACGGGGGAAGCGCGACGTTACGCGCTCACATACCATGGCATTCTTCGTTGCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGc                                                                                                                     <  2:54546/149‑1 (MQ=255)
                               gggTGAAGCGCGACGTTACGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGTAGGCCGGCTAACGCGGTAAGTCTataa                                                                                                               >  1:343172/1‑149 (MQ=255)
                                  tGAAGCGCGACGGTACGCGCTCACTTACCGTCGGATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAAc                                                                                                            <  2:392857/149‑1 (MQ=255)
                                                    gcTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGc                                                                                                                                          <  1:88873/101‑1 (MQ=255)
                                                    gcTAACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGc                                                                                                                                          >  2:88873/1‑101 (MQ=255)
                                                     cTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAACAAACATGTTCATGCCTGGCCCgg                                                                                         >  2:439612/1‑149 (MQ=255)
                                                                   cATGCGGCGTCTCGCCCTTTAATACCGGAATTTGATGAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTCTCAGaa                                                                           <  2:364004/149‑1 (MQ=255)
                                                                         gcGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAg                                                                     <  1:476961/149‑1 (MQ=255)
                                                                            tctcTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTaa                                                                                                                               >  1:323407/1‑88 (MQ=255)
                                                                            tctcTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTaa                                                                                                                               <  2:323407/88‑1 (MQ=255)
                                                                              ttttCCTTTAAGACCGGAATTTGATCTGCACGCGTGATATCTTCTACAGCGAATGTCTGGCCTGCTATTGCCAGCAGGCCAGCTAACGCGGTAAGCCTAAATAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGtt                                                                <  2:123829/147‑1 (MQ=255)
                                                                                           ccGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCGTCCGTTTCAGAACACCAGGCGGTCTTCGCGCACAAt                                                   >  1:296325/1‑149 (MQ=255)
                                                                                           ccGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAAt                                                   >  2:45076/1‑149 (MQ=255)
                                                                                            cGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATc                                                  >  1:85977/1‑149 (MQ=255)
                                                                                                     aTCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATa                                         >  2:39947/1‑149 (MQ=255)
                                                                                                     aTCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGGAAGCCTAAAAAACATGTTCATGCCTGTCTCGGCCTCCGATTCAGTAGACCAGGTGTTCTGAGAGCACACTCAAGGACATa                                         >  1:354856/1‑149 (MQ=255)
                                                                                                      tCAGGACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATAc                                        <  1:259046/149‑1 (MQ=255)
                                                                                                      tCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATAc                                        >  1:306915/1‑149 (MQ=255)
                                                                                                         gCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAg                                                                             >  1:11278/1‑109 (MQ=255)
                                                                                                         gCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAg                                                                             <  2:11278/109‑1 (MQ=255)
                                                                                                                                gAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCg              >  1:385892/1‑149 (MQ=255)
                                                                                                                                 aaGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGt             <  2:482255/149‑1 (MQ=255)
                                                                                                                                    gTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCtt          >  1:245580/1‑149 (MQ=255)
                                                                                                                                         gCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCACAGACATACCCGGAGTCAGCTGGACGCGGACGCCGTCTTTGGTg     >  1:222119/1‑149 (MQ=255)
                                                                                                                                           cTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCACTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCACCTGGACGCGGACGCCGTCTTTGGTgct   >  2:189596/1‑147 (MQ=255)
                                                                                                                                            tGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCaa                                                <  2:179746/103‑1 (MQ=255)
                                                                                                                                            tGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCaa                                                >  1:179746/1‑103 (MQ=255)
                                                                                                                                            tGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGAtt  >  1:154957/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
TGATCGAGGTCGAACTGGCGATAATGAGAACGGGTGAAGCGCGACGTTACGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATT  >  NC_000913_3_pae_tpiA/1914626‑1914914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.