Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	2,795,396	C→A	Q382K (CAG→AAG)	gabP →	gamma‑aminobutyrate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	2,795,396	0	C	A	100.0%	62.5 / NA	22	Q382K (CAG→AAG)	gabP	gamma‑aminobutyrate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/8);  total (14/8)

CGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCT‑GCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAAC  >  NC_000913_3_pae_tpiA/2795253‑2795534                                                                                                                                                 |                                                                                                                                           cGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCT‑GCGCAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTCGTCATCGCCGTTTCAAAGTTg                                                                                                                                       >  2:172370/1‑149 (MQ=255)               ttACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCT‑GCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGCTGCGCAAAAt                                                                                                                         >  2:408863/1‑149 (MQ=255)                    ctctACCGGCGCGTCATTCTTAATTGTTGTGGTGACGTCTTGCG‑GCCTGGTGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGc                                                                                                                    <  1:40028/149‑1 (MQ=255)                                       ttAACGGTGGTGGTGAACTATTACGCGCCT‑GCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTTCGGATGCGCAAAATTCTGCGACCAAAAGGAAGCGAAAt                                                                                                 >  2:4326/1‑149 (MQ=255)                                       ttAACGGTGGTGGTGAACTATTACGCGCCT‑GCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAAt                                                                                                 >  1:323652/1‑149 (MQ=255)                                       ttAACGGTGGTGGTGAACTATAACGCGCCT‑GCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAAt                                                                                                 >  2:454338/1‑149 (MQ=255)                                            ggtggtggtGAACTATTACGCGCCT‑GCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTc                                                                                               <  2:26295/146‑1 (MQ=255)                                            ggtggtggtGAACTATTACGCGCCT‑GCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTc                                                                                               >  1:26295/1‑146 (MQ=255)                                                         tATTACGCGCCT‑GCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCt                                                                               >  2:70208/1‑149 (MQ=255)                                                               gcgcCC‑GCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCCGAAGGAAGCGAAATTCGCTTGCGAAAGTGGCTTTATcc                                                                         >  1:371335/1‑149 (MQ=255)                                                                                ttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACTTGGCTa                                                         >  2:406450/1‑149 (MQ=255)                                                                                      ttCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATc                                                   <  2:269336/149‑1 (MQ=255)                                                                                              gACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTCGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTttat                                           >  2:451303/1‑149 (MQ=255)                                                                                                      gggCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGGGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTtg                                   >  2:392566/2‑149 (MQ=255)                                                                                                        gcgcTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATACGCTTGCGAATGTGTCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTtgtg                                 <  1:454338/149‑1 (MQ=255)                                                                                                           cTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTg                              <  1:85187/149‑1 (MQ=255)                                                                                                            tATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTgg                             <  1:319798/149‑1 (MQ=255)                                                                                                                 cccTGCTGGCTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTATCTTTGTGTTGGTAGTg                        <  1:449480/149‑1 (MQ=255)                                                                                                                  cgtggttgTTTATTGAGGCATCTGCGGTTCAAAGTTGCGGATGGGCAAAATTCTGCGAGCAGCAGGAAGCGTAATTCGCTGGCGAATGTGGCTTTTTCCGTGGATCACCTGGCTAGTCCTCGGCTTTATTACCTTTGTGTTGGTAGTGa                       <  2:67819/142‑1 (MQ=255)                                                                                                                              tttaGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATtccgtc           >  2:6935/1‑149 (MQ=255)                                                                                                                                     aacGCCGTTTCAAAGTAGCGGATGCGAACAACGAAGCAGGCAGAAGGAAGCGAAATTCGCTTGCGAATATGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGTCTTTATTACCTCTGTGTTGGTCCTGATCCTTTTCCGTTCggaagc    >  2:112138/3‑145 (MQ=255)                                                                                                                                       cGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAAc  >  2:421251/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                           CGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCT‑GCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAAC  >  NC_000913_3_pae_tpiA/2795253‑2795534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑