Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,184,269 A→C T1037P (ACC→CCC)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,184,2690AC96.3% 84.6 / NA 27T1037P (ACC→CCC) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (14/12);  minor base G (1/0);  total (15/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATC  >  NC_000913_3_pae_tpiA/4184124‑4184406
                                                                                                                                                 |                                                                                                                                         
gCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACCAAATAAGTCGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCcccc                                                                                                                                        >  1:469216/1‑149 (MQ=255)
 cTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCa                                                                                                                                       >  2:330265/1‑149 (MQ=255)
       aaaCTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGcga                                                                                                                                 >  1:336345/1‑149 (MQ=255)
         aCTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTTGCTGGGCAGTATGACGAACTGAAACACGAGATCGAGAAGAAACTCGAAGCGAACAGCCGCAAAATCCCCCAGGGcgacg                                                                                                                               >  2:95373/1‑149 (MQ=255)
         aCTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGcgacg                                                                                                                               >  1:200910/1‑149 (MQ=255)
            gCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATc                                                                                                                            <  2:342414/149‑1 (MQ=255)
                     tCGCTGGCTGGAGCTGGGCCTGAAAGACGAAGAGAAACAAAATCAGCTGGCACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCgg                                                                                                                   <  1:332245/149‑1 (MQ=255)
                             tggAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTg                                                                                                           >  1:14900/1‑149 (MQ=255)
                               gAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGaa                                                                                                         <  1:330265/149‑1 (MQ=255)
                                 gCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGa                                                                                                       >  1:330930/1‑149 (MQ=255)
                                     ggCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCgg                                                                                                                   >  1:109424/1‑133 (MQ=255)
                                     ggCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCgg                                                                                                                   <  2:109424/133‑1 (MQ=255)
                                         tGACAGACGAAGAGAAACAAAATGAGCTGGAACAGCTGGCTGAGCAGGATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAg                                                                                               <  2:21781/149‑1 (MQ=255)
                                             aGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGcga                                                                                                                                 <  1:38047/111‑1 (MQ=255)
                                             aGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGcga                                                                                                                                 >  2:38047/1‑111 (MQ=255)
                                               aCGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCACCGATCTGGCACCGGGCGTGCTGAAGATTGTTACGGTatat                                                                                         >  2:95838/1‑149 (MQ=255)
                                                        aaCAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGtt                                                                                >  1:250151/1‑149 (MQ=255)
                                                               tCAGTTGGAACGGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGACCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGcc                                                                         <  2:469216/149‑1 (MQ=255)
                                                               tCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGCAGATTGTTAAGGTATATCTGGCGGTTAAACGcc                                                                         >  2:202414/1‑149 (MQ=255)
                                                                      gAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCa                                                                  <  1:279038/149‑1 (MQ=255)
                                                                                          tGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAg                                              <  2:14900/149‑1 (MQ=255)
                                                                                          tGACGAACTGAAACACAAGTGCGAGAAGTAACTCGAAGCGAAACGCCGCAAGATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAg                                              <  2:112671/149‑1 (MQ=255)
                                                                                                  tGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCGACCAGGGCGACGATCTGGAACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCAc                                      >  1:40278/1‑149 (MQ=255)
                                                                                                       cacGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGGTGGCAGGTCGTCACGGTaa                                 >  1:43242/1‑149 (MQ=255)
                                                                                                            gTTCGAGAAGAAACTTGAAGCGAAACGCCGCAAAACTCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAgg                            <  2:40278/149‑1 (MQ=255)
                                                                                                              tCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCGCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGgt                          <  2:464200/149‑1 (MQ=255)
                                                                                                                          tCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAg              >  1:212796/1‑149 (MQ=255)
                                                                                                                                      gccgcAAAATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATc  >  1:450944/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                         
GCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATC  >  NC_000913_3_pae_tpiA/4184124‑4184406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑