Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 2,795,396 C→A Q382K (CAG→AAG)  gabP → gamma‑aminobutyrate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,795,3960CA98.6% 231.3 / NA 72Q382K (CAG→AAG) gabPgamma‑aminobutyrate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (27/44);  minor base G (0/1);  total (27/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAGA  >  NC_000913_3_pae_tpiA/2795255‑2795541
                                                                                                                                             |                                                                                                                                                 
tagtgggCGGTGTTACTCTCCACCGGCGCGGCATTCTTACCGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCg                                                                                                                                            <  2:1320293/144‑1 (MQ=255)
                tctcatccGGCGGGGAATTCTGAGCTGTAGACGTGAACTATCACGCGCCTGCGAAGGTATTTAATTTTCTTATCAACAGCTCCGGCGTTATCGGCCTGCTGGTTTAGTTAGTCATCGCCGTTTCAGCGGTGCGGTTGCGCAAAATTTTg                                                                                                                            <  2:1442293/143‑1 (MQ=255)
                tctcCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCGCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTg                                                                                                                            <  2:1442370/149‑1 (MQ=255)
                tctcCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTg                                                                                                                            >  1:726520/1‑149 (MQ=255)
                 ctcCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTCCGCGCCTGCGAAGGTATTTAAATTTCGTATCAAGAGCTCCTGCTCTCGGTCGCCGCTGGATTATTAAGTCATAGAGCTTTCAAACTTGCGGATGCGCAAACTTcaga                                                                                                                           >  2:1071042/1‑146 (MQ=255)
                 ctcCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGc                                                                                                                           <  2:1260586/149‑1 (MQ=255)
                  tcCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCg                                                                                                                          <  2:1268222/149‑1 (MQ=255)
                      ccGGCGCGGCATTCTTAACGGTGGTCGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGcc                                                                                                                      >  2:376658/1‑148 (MQ=255)
                         gcgcGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAgaa                                                                                                                   <  1:1056663/149‑1 (MQ=255)
                             ggCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGATTGAAGTTACATTCCTCGGCAACAACGCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAACATTCTGCGAGCAgaaggaa                                                                                                               >  2:1388880/1‑149 (MQ=255)
                              gCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGTTTTATTTAGTCATCGCCGTTGCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAgaaggaag                                                                                                              <  1:580452/149‑1 (MQ=255)
                               cATTCTTAAGGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTTGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGc                                                                                                             <  2:443922/149‑1 (MQ=255)
                               cATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGc                                                                                                             >  1:828023/1‑149 (MQ=255)
                                    ttAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAAt                                                                                                        <  1:61024/149‑1 (MQ=255)
                                     tAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAAtt                                                                                                       <  2:890060/149‑1 (MQ=255)
                                      aaCGGTGGTGGTGAACTATTACGCGCCTGCGAATGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTc                                                                                                      <  2:1303718/149‑1 (MQ=255)
                                          gtggtggtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCtt                                                                                                  <  2:636993/149‑1 (MQ=255)
                                            ggtggtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCTCTTGc                                                                                                >  2:385109/1‑149 (MQ=255)
                                                 tgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCACTGTTTCAAAGTTGAGGATGCGCAAAATTCTGCGCGCAGAAGGAAGCGAAATTCGCTTGCGAAta                                                                                           >  1:698948/1‑148 (MQ=255)
                                                  gAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGc                                                                                                                           >  1:1316365/1‑116 (MQ=255)
                                                  gAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGc                                                                                                                           <  2:1316365/116‑1 (MQ=255)
                                                  gAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAAtgt                                                                                          <  1:962195/149‑1 (MQ=255)
                                                  gAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTATGCGAGCAGAAGGAAGAGACATTCGCTTGCGAAtgt                                                                                          >  2:948966/1‑149 (MQ=255)
                                                      tATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCt                                                                                      <  2:849027/149‑1 (MQ=255)
                                                      tATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCt                                                                                      <  1:532525/149‑1 (MQ=255)
                                                      tATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCt                                                                                      >  1:453630/1‑149 (MQ=255)
                                                      tATTACGCGCCTGCGAAGGTATCTAAATTTCTGATCGACAGCTCCGGCGCTATCGCACTGCTGGTTTAATTAGTCATCGCCGTATCAAAGTTGCGGATGCGCAAAATTCTACGAGCAGAACGAAGCGAAATTCGCTTACTAATGTGGCt                                                                                      >  2:1005286/1‑149 (MQ=255)
                                                      caTTACCCGCCTGCTAAGGTATTTATATTTCTTATCGACAGCTCCGGCGCTATCGCCCTTCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCt                                                                                      <  2:617518/148‑1 (MQ=255)
                                                              gcCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGt                                                                              >  1:470657/1‑149 (MQ=255)
                                                               ccTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTg                                                                             <  1:666409/149‑1 (MQ=255)
                                                                 tGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAGGCGAACTTCGCTTGCGAATGTGGATTTATCCGTGGc                                                                           >  2:119014/1‑149 (MQ=255)
                                                                         tATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTATTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTgg                                                                   <  1:52922/149‑1 (MQ=255)
                                                                          aTTTAAATTTCTGATCGACGGCTCCGGTGCTATCGCCCTGCTGGTTTATTTAGGCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGc                                                                  <  2:539031/149‑1 (MQ=255)
                                                                          aTTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGc                                                                  <  2:1225737/149‑1 (MQ=255)
                                                                           tttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTAACCTGGCt                                                                 >  2:685039/1‑149 (MQ=255)
                                                                               aaTTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATAGCCGATTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTc                                                             >  1:579802/1‑149 (MQ=255)
                                                                                     tGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGc                                                       <  1:1217149/149‑1 (MQ=255)
                                                                                          gACAGGCCCGGCGTCATCCTCCCGCTCGTTTATTTCGTCCTCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTttat                                                  <  1:1032734/149‑1 (MQ=255)
                                                                                          gACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCCAACTACACCTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTttat                                                  <  2:238059/149‑1 (MQ=255)
                                                                                          gACAGCTCCAGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTttat                                                  >  1:562099/1‑149 (MQ=255)
                                                                                                tCCGGCGCTATCGCCCTGCTGCTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt                                            <  1:683011/149‑1 (MQ=255)
                                                                                                   ggCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTtgt                                         <  1:298231/149‑1 (MQ=255)
                                                                                                   ggCGCTATCGCCCTGACGGATTACTGAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTtgt                                         >  2:680713/1‑149 (MQ=255)
                                                                                                     cgcTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCg                                                                                                                                            <  1:710862/48‑1 (MQ=255)
                                                                                                     cgcTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCg                                                                                                                                            >  2:710862/1‑48 (MQ=255)
                                                                                                       cTATCGCCCTGCTGGTTTATTGAGCCAGCGCTGTTTCAAAGTTGCGGATGCGCAAACTTCTGCGAGCAGAAGGACGCGAACTTCGCTTGCGAATTTGGCTTTATCCGTCTCTCCCCTCGCTAGTCCTCTGATTTATGTATTTTGTGTTg                                     <  2:1116225/149‑1 (MQ=255)
                                                                                                          ccccccTGCTGACTTATTTAGTCACCGCCGTTTCAAAGTTGCGGATCCCAAAAACTCTCCGAGCAGAAGGAAGCGAAATTCGCGTGCGAATGTGCCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTa                                  <  2:30024/146‑1 (MQ=255)
                                                                                                            gCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGt                                <  1:791177/149‑1 (MQ=255)
                                                                                                               ctgctgGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGAt                             <  1:1180364/149‑1 (MQ=255)
                                                                                                                 gctgGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc                                                                       <  1:90350/105‑1 (MQ=255)
                                                                                                                 gctgGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc                                                                       >  2:90350/1‑105 (MQ=255)
                                                                                                                     ggttatttaGTCATCGCCGTTTCAAAGTTCCGGATGCGCAAACTTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCTGTTGTTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTAtt                       <  1:704719/147‑1 (MQ=255)
                                                                                                                     gTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAGGCGAAATTCGCTTGCGAAt                                                                                            >  1:1423733/1‑80 (MQ=255)
                                                                                                                     gTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAAt                                                                                            <  2:1423733/80‑1 (MQ=255)
                                                                                                                     gTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTAtt                       <  2:77172/149‑1 (MQ=255)
                                                                                                                       ttatttaGTCATCGCCGTTTCAAAGTTGCGGAGTCGCAAAATGCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATtcc                     <  2:1383382/149‑1 (MQ=255)
                                                                                                                        tatttaGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTgg                                    <  2:616590/133‑1 (MQ=255)
                                                                                                                        tatttaGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTgg                                    >  1:616590/1‑133 (MQ=255)
                                                                                                                          tttaGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATtccgtc                  <  1:791728/149‑1 (MQ=255)
                                                                                                                           ttaGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGATAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATtccgtcc                 >  1:835034/1‑149 (MQ=255)
                                                                                                                            tagctcgcGCCGTTTCAAAGGTGCGTATGCGCAAAATTCTGCGAGTAGAAGTAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCGTATCTGGTTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATtccgtccg                <  2:40923/142‑1 (MQ=255)
                                                                                                                              gTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTgg                                    >  2:985092/1‑127 (MQ=255)
                                                                                                                              gTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTgg                                    <  1:985092/127‑1 (MQ=255)
                                                                                                                                  tCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCaa          >  1:1436618/1‑149 (MQ=255)
                                                                                                                                    gCCGTTTGAAAGTTGCGGATGCCCACGATGCGGCAGGTAGAATGAAGCGAAATTCGCTTGCGAATGTCGGTTTATCCGTGGCTCACCTGGCTAGTCACCGGCTTTATTACCTTTGTTTTGGTAGTGATGCTATTCCGTCCGGCGCAACa        <  2:662677/149‑1 (MQ=255)
                                                                                                                                     ccGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGACATTAGCTTTCGAATGTGGCTTTATCCGTGGCACACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAg       >  2:1248171/1‑149 (MQ=255)
                                                                                                                                     ccGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTTCCAGGCCTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAg       >  1:40736/1‑149 (MQ=255)
                                                                                                                                      cGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGCAATTCGCTTTCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGt      <  2:726520/149‑1 (MQ=255)
                                                                                                                                      cGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGt      <  1:1360946/149‑1 (MQ=255)
                                                                                                                                      cGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGCCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGt      <  1:1361167/149‑1 (MQ=255)
                                                                                                                                          tCAAAGTTGCGGATGCGCAAAATTCTGCGAGCCGAAGGAAGCGAAATTCGCATGCGAATGGAGCTTTATCCCTGGCTCACCTGGCTAGCCATCGACTTTATTACCTTTGTGTTGCTAGTGAGGCTATTCCGTCCGGCGCAACCGTTCGa  >  2:368406/1‑149 (MQ=255)
                                                                                                                                          tCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAGa  <  2:453630/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
TACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAGA  >  NC_000913_3_pae_tpiA/2795255‑2795541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑