Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	2,876,388	C→A	intergenic (‑59/‑193)	cysD ← / → iap	sulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	2,876,388	0	C	A	100.0%	269.0 / NA	86	intergenic (‑59/‑193)	cysD/iap	sulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (44/42);  total (44/42)

TTTGAGAATTCTGCCGCCACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTT  >  NC_000913_3_pae_tpiA/2876244‑2876517                                                                                                                                                 |                                                                                                                                  tttGAGAATTCTGCCGCCACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTaa                                                                                                                               >  2:919156/1‑149 (MQ=255)   tGAGAATTCTGCCGCCCCCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGGCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAaga                                                                                                                             <  2:1094337/149‑1 (MQ=255)   tGAGAATTCTGCCGCCACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAaga                                                                                                                             <  1:945276/149‑1 (MQ=255)    gagaATTCTGCCGCCACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGGGAGTAcgag                                                                                                                            >  2:106905/1‑149 (MQ=255)    gagaATTCTGCCGCCACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAagag                                                                                                                            <  1:1161703/149‑1 (MQ=255)     agaATTCTGCCGCCACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAgaga                                                                                                                           >  1:1107304/1‑149 (MQ=255)     agaATTCTGCCGCCACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAgaga                                                                                                                           >  2:216637/1‑149 (MQ=255)      taaTTCCGCCGACACCCCGCTTATCATGTGGATGCTTTCCGCATCCAGTTGCCGCATGTTTGTAAGTCGTATTTGATCCATAACCGTTCCATTGCAATACCGCAATTTTCTTGCCATCAGATGTTTCGAATATAGAGAGAGTAAGAGaa                                                                                                                          <  2:624358/148‑1 (MQ=255)      gaATTCTGCCGCCACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGaa                                                                                                                          <  2:1062506/149‑1 (MQ=255)      gaATGCCGCCGCTACCTCGCGAGTAATGGGGATGCTTTGCGTCTCCAGTTGCCGCAGGTGAGTAAGGCGTATTTGATCCATAACCGTTCCTTTGCAATGCCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGaa                                                                                                                          <  2:149697/149‑1 (MQ=255)       aaTTCTGCCGCCACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAAc                                                                                                                         <  1:761360/149‑1 (MQ=255)              ccgccACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGaa                                                                                                                  <  1:1082009/149‑1 (MQ=255)               cgccACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGaaa                                                                                                                 <  1:228881/149‑1 (MQ=255)                gccACCTCGCGAATAATGTGGATGCTTGCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAAt                                                                                                                <  2:654205/149‑1 (MQ=255)                 ccACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAAtt                                                                                                               <  2:174220/149‑1 (MQ=255)                     cTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCa                                                                                                           <  2:1436085/149‑1 (MQ=255)                      tCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCTTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCaa                                                                                                          >  1:522102/1‑149 (MQ=255)                         cgAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTa                                                                                                       <  1:986394/149‑1 (MQ=255)                          gAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCATTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAAAGAACGAATGAAATTACCAATTAg                                                                                                      >  2:1016774/1‑149 (MQ=255)                           aataatGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGa                                                                                                     >  2:1331371/1‑149 (MQ=255)                            ataatGTGGAGGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGaa                                                                                                    <  1:1283935/149‑1 (MQ=255)                             taatGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAAt                                                                                                   <  1:216637/149‑1 (MQ=255)                               atGTTGATGCTTTCCTCCTCCAGTTTCCGCAGGTGAGTTAGTCGTATTTGATGCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGa                                                                                                 <  1:996567/149‑1 (MQ=255)                                  tgGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGGGAGTAAGAGAAGGAATGAAATTACTAATTAGAATGagta                                                                                              >  2:346447/1‑149 (MQ=255)                                    gATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGagtagt                                                                                            >  1:389592/1‑149 (MQ=255)                                    gATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGagtagt                                                                                            >  2:302582/1‑149 (MQ=255)                                     aTGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTTGACTATAGGGAGAGGAAGAGAACGAATGAAATTACCAATTAGAAGGAGTGGtt                                                                                           >  1:360981/1‑149 (MQ=255)                                           tCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTaa                                                                                     <  1:857649/149‑1 (MQ=255)                                             cGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACg                                                                                   <  1:514527/149‑1 (MQ=255)                                             cGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTACCATCAGATGTTTCGACTATAGCGAAAGTAAGAGAATGAATGAAATTACCAATGAAAATGAGTAGTTCCACAACg                                                                                   >  2:763146/1‑149 (MQ=255)                                              gCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACgg                                                                                  >  1:305540/1‑149 (MQ=255)                                               ccTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATATTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGa                                                                                 >  2:1246199/1‑149 (MQ=255)                                                cTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGaa                                                                                >  1:510428/1‑149 (MQ=255)                                                cTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGaa                                                                                >  1:194203/1‑149 (MQ=255)                                                cTCCAGTTGCCGCAGGTGAGTAAGGCGTATTTGATCCATTTCCGTTCCTTTGGAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGaa                                                                                <  1:659524/149‑1 (MQ=255)                                                 tCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAAc                                                                                    >  2:852532/1‑144 (MQ=255)                                                 tCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAAc                                                                                    <  1:852532/144‑1 (MQ=255)                                                 tCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAAt                                                                               >  1:86123/1‑149 (MQ=255)                                                 tCCAGTTGCCGCAGGTGAGTAAGTCGTAAGTTCGCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTTCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAAt                                                                               >  2:708659/1‑149 (MQ=255)                                                  ccAGTTTGCACATGTGAGTAAGTCGTATTTGTTCCATAACCGGTCCTCTGTAATACCGCTATTTTCTGGCCATCAAATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATa                                                                              <  2:45021/149‑1 (MQ=255)                                                   cAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTCGTTCCTTAACGGACTaa                                                                             >  1:1109297/1‑149 (MQ=255)                                                   cAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATaa                                                                             <  2:389592/149‑1 (MQ=255)                                                          cGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTg                                                                      >  1:1413382/1‑149 (MQ=255)                                                              ggTGAGTGAGTGGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCaa                                                                  <  1:1081692/149‑1 (MQ=255)                                                              ggTGAGGAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACAGCTAACTGATTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCaa                                                                  <  1:1129715/149‑1 (MQ=255)                                                               gTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCaaa                                                                 <  2:1413382/149‑1 (MQ=255)                                                                 gAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCCGATGTTTCGACTATAGGGAGAGTAAGAGAAAGACTGAAATTACCAATTAGAATGAAGAGTTCCTTACAGGTATAACGATTTGGCAAAGc                                                               >  2:424727/1‑149 (MQ=255)                                                                    tAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCATTTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTaa                                                            >  2:1397588/1‑149 (MQ=255)                                                                    tAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTaa                                                            >  1:1075513/1‑149 (MQ=255)                                                                    tAAGTCGTATTTGATCCATAACCGTACCTTTGCAATACCGCTATTTTCTTGCCATCAGATTGCCCGACTAGATGTTCACTAGTACAAAGAATGAAATTAGCAATGAGAATGACTAGTTCCTTACAGGCATCACGATTTGCAAAAGCTaa                                                            >  2:364430/1‑149 (MQ=255)                                                                     aaGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAat                                                           <  2:194203/149‑1 (MQ=255)                                                                      aGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAata                                                          <  1:302582/149‑1 (MQ=255)                                                                        tCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATG‑TTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCa                                                       >  2:1379104/1‑149 (MQ=255)                                                                                  gccATTACCGTTCCTTTGCAATACCTCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCt                                              <  1:685996/148‑1 (MQ=255)                                                                                    cATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCtt                                                                                       <  2:221951/106‑1 (MQ=255)                                                                                    cATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCtt                                                                                       >  1:221951/1‑106 (MQ=255)                                                                                    cATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGACTTGGCAAAGCTAATATCAAAAAGTGCTTa                                            >  2:140492/1‑149 (MQ=255)                                                                                      tAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAg                                          <  1:1331371/149‑1 (MQ=255)                                                                                      tAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATGAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAg                                          <  2:1051971/149‑1 (MQ=255)                                                                                       aaCCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAgg                                         >  2:1151971/1‑149 (MQ=255)                                                                                       aaCCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAgg                                         >  1:1275302/1‑149 (MQ=255)                                                                                          cGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATACGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAACTGCTAAACGCAc                                      >  2:900764/1‑149 (MQ=255)                                                                                          cGTTCCTTTGCAATACCGCTATTTTCTTGCCATCACATGTTTCGACTATAGGGAGAGTAAGAGAACGAATCAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCAc                                      <  1:1334069/149‑1 (MQ=255)                                                                                           gTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCAGTCACGAAATAACAATTTGGCAAGGCTAATATCAAAAAGTGCTTAAGGCAcc                                     >  2:1364649/1‑149 (MQ=255)                                                                                                tttGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGAtt                                >  2:16583/1‑149 (MQ=255)                                                                                                  tGCAATACCGCTCACTTCTTGCCATCAGATGTTTCGACTCTAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGAATTGGCAAACCTAATATCAAAAAGTGCTTAAGGCACCGGATTTc                              <  1:608188/149‑1 (MQ=255)                                                                                                    cAATACCGATAATTTCTTTCCATCAAATCATTTGACTATAAGGAGATTAAGAGAACTAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGTCACCGGATTTCgg                            <  2:888817/149‑1 (MQ=255)                                                                                                     aagaCCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCACAGCTAAGATCAAAAAGTGCTTAAGGCACCGGATTTCggg                           <  1:856318/146‑1 (MQ=255)                                                                                                     aaTACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCggg                           <  1:1364649/149‑1 (MQ=255)                                                                                                        aCCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGc                                               >  1:1163461/1‑126 (MQ=255)                                                                                                        aCCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGc                                               <  2:1163461/126‑1 (MQ=255)                                                                                                          cGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTAGGCACTAGAAGGAGTTGGCTCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGttt                      >  1:1421774/1‑149 (MQ=255)                                                                                                          cGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGttt                      <  1:968855/149‑1 (MQ=255)                                                                                                          cGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCGTAAGGGATCGGATGTCGGGCGttt                      >  2:759145/1‑149 (MQ=255)                                                                                                           gCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAGTATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTa                     >  1:894564/1‑149 (MQ=255)                                                                                                            cTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGAGCGTTTAg                    >  2:261270/1‑149 (MQ=255)                                                                                                             tATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAgg                   >  1:218223/1‑149 (MQ=255)                                                                                                               gtgtCTTGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCAAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGaa                 >  2:301156/4‑149 (MQ=255)                                                                                                                ttacatGCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAg                <  1:611934/144‑1 (MQ=255)                                                                                                                 ttCTTGCCATTAAATTTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGa               <  1:228308/149‑1 (MQ=255)                                                                                                                      gCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGaa                                                                                                                      >  2:1312138/1‑41 (MQ=255)                                                                                                                      gCCATCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGaa                                                                                                                      <  1:1312138/41‑1 (MQ=255)                                                                                                                         aTCAGATGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTGCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAt       >  1:1253858/1‑149 (MQ=255)                                                                                                                              aTGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGttt  <  1:1151971/149‑1 (MQ=255)                                                                                                                              aTGTTTCGACTATAGGGAGAGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGttt  >  1:988368/1‑149 (MQ=255)                                                                                                                              aTGTATCCACTATAGGGAGAGTATGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTGACGGACTAACGATTTGGCAAAGCTAATATCAAAAAGTGCTCAAGGCACCGAATTTCGGACGTTTAGGAGGATTTGAGATTGttt  >  2:1408104/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                  TTTGAGAATTCTGCCGCCACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTT  >  NC_000913_3_pae_tpiA/2876244‑2876517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑