New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 2724404 =22 (0.670)5 (0.160) 5/270 3.8 17.9% coding (277/324 nt) yfiM putative lipoprotein
?NC_000913_3_pae_tpiA 2724422 = 25 (0.800)coding (295/324 nt) yfiM putative lipoprotein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGA  >  NC_000913_3_pae_tpiA/2724277‑2724564
                                                                                                                                                 |                                                                                                                                              
gATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCtata                                                                                                                                             <  1:202328/149‑1 (MQ=255)
                     cATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGAAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCGAGCACCGGCTATACCGTCTGGCAACTGACCCGTc                                                                                                                        >  2:60967/1‑149 (MQ=255)
                      aTGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCa                                                                                                                       >  1:204617/1‑149 (MQ=255)
                           ttgagggATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTaaa                                                                                                                  <  2:404780/143‑1 (MQ=255)
                                taaTGCTCTCTGTCAGCGTGGCGGCGTCAAAAGAGCTTTGGGATACCCCCCCCGAAGGGAGCGGCTGGGGCTGGAAGGATTTGGCCTGGGATGTCGCCGCTGCAAGCACCGTCTATACCGTCTGGCACTTGACCCGTCACCAAGTACGc                                                                                                             <  2:132138/147‑1 (MQ=255)
                                   tGTTCTCTGTCAGTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTCTGCCTGGGATGTGCCCGGTGCAAGCACCGGCTATACCCTCTTGCAACTGATTTGTCATTAAAGACGCATc                                                                                                          <  2:442792/149‑1 (MQ=255)
                                     ttCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTg                                                                                                                                      <  1:95620/119‑1 (MQ=255)
                                     ttCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTg                                                                                                                                      >  2:95620/1‑119 (MQ=255)
                                                             aaaGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCTATGTAGCAtaca                                                                                >  1:151305/1‑146 (MQ=255)
                                                                                    cGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACgg                                                         <  1:93450/149‑1 (MQ=255)
                                                                                              gggtggagcgagaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGt                                               <  2:166064/146‑1 (MQ=255)
                                                                                                       tggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGAAGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAAACAGAAACGCCACCACGGGCATCCAGGTCACATACCa                                      >  1:184178/1‑149 (MQ=255)
                                                                                                         gaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTACAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCCCATACCaca                                    >  2:42162/1‑147 (MQ=255)
                                                                                                         gaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCaga                                    >  2:40420/1‑149 (MQ=255)
                                                                                                         gaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCaga                                    >  1:489214/1‑149 (MQ=255)
                                                                                                         gaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCaga                                    >  1:490351/1‑149 (MQ=255)
                                                                                                          aaGGATTTGGCCTGGGATGTCGCCAGTGCAAGCACCGGCTATACCTTCTGGCAACTCACCAGTCACTAAAGACGAATCCCCTACCCTTTGCGATGAAACATCAAACAAACCAGAAAAACCAACCCGACCATCAAGGGAACAACCCagaa                                   >  2:246153/1‑149 (MQ=255)
                                                                                                           aGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCagaag                                  >  1:285116/1‑149 (MQ=255)
                                                                                                              aTTTGGCCGGGGATGTCGGCGCTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGaagaag                               <  2:88316/149‑1 (MQ=255)
                                                                                                               tttGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAgg                              <  1:498922/149‑1 (MQ=255)
                                                                                                               tttGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAgg                              <  1:171555/149‑1 (MQ=255)
                                                                                                                               gCCGGTGCAAGCACCGGCTATACCGTCTGGAAACTGACGCGTCACTAAAGACGCATCACATTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTAt              >  2:76409/1‑149 (MQ=255)
                                                                                                                                ccGGTGCTTGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTAtt             >  1:13449/1‑149 (MQ=255)
                                                                                                                                ccGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATAAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTGCCATACCTGtt             >  1:87989/1‑149 (MQ=255)
                                                                                                                                       ttgCACCGGCTATACCGTCTGGCAACTGACCCGTCACTaaa                                                                                                                  <  1:538558/39‑1 (MQ=255)
                                                                                                                                       ttgCACCGGCTATACCGTCTGGCAACTGACCCGTCACTaaa                                                                                                                  >  2:538558/3‑41 (MQ=255)
                                                                                                                                       aaGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTc                  >  1:456249/1‑137 (MQ=255)
                                                                                                                                       aaGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTc                  <  2:456249/137‑1 (MQ=255)
                                                                                                                                           aCCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGa  <  2:514493/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
GATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGA  >  NC_000913_3_pae_tpiA/2724277‑2724564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.