Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 264,098 G→T L50L (CTC→CTA)  ykfH ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA264,0980GT98.0% 139.5 / NA 51L50L (CTC→CTA) ykfHuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (23/27);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTA  >  NC_000913_3_pae_tpiA/263957‑264233
                                                                                                                                             |                                                                                                                                       
tCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTCCAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGGATACCGCTAGGACTc                                                                                                                                  >  1:245903/1‑149 (MQ=255)
tCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCCCTATTGTCAGGTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTc                                                                                                                                  >  2:441313/1‑149 (MQ=255)
    aGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGc                                                                                                                              <  2:509474/149‑1 (MQ=255)
                aTCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAAc                                                                                                                  >  2:250224/1‑149 (MQ=255)
                     ccGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGc                                                                                                                              >  1:292486/1‑132 (MQ=255)
                     ccGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGc                                                                                                                              <  2:292486/132‑1 (MQ=255)
                          gcgggCCCCTGGTAGGGTTGCTCATTACAGGTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCg                                                                                                        <  2:619943/146‑1 (MQ=255)
                             ggCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                                                                                                     <  1:216498/149‑1 (MQ=255)
                              gCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtgt                                                                                                    >  1:78879/1‑149 (MQ=255)
                              gCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGACGATATCCGGAACGTCCCTGCCGgggt                                                                                                    >  1:117626/1‑149 (MQ=255)
                                cccTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGGGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATGCGGGACGTCCCTGGCTGTGTag                                                                                                  >  1:537563/1‑148 (MQ=37)
                                cccGGGGAGGGGTGTTTATTATAGTTCTCCACGACTGTCAGTTCAGGGTCATGGGCAACAGGCAGGTATAGGGCCCATGGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTaa                                                                                                  <  2:346380/149‑1 (MQ=255)
                                 ccTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCACATTGCGGTCCGGGCGGCGTTCGGCGTATACCGGTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAAt                                                                                                 >  1:573579/1‑149 (MQ=255)
                                       aGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGa                                                                                           <  1:250224/149‑1 (MQ=255)
                                        gggTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGAc                                                                                          <  1:595538/149‑1 (MQ=255)
                                         ggTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGCTATCCGGCACGTGCCTGCCGGAGTAATGACAGCCg                                                                                         >  2:31383/1‑149 (MQ=37)
                                                ctttACAGTTCTCCAGTATTGTCAGTTCAGGGTGATGCTCATAAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGGTAGGCCTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGt                                                                                  <  1:197906/147‑1 (MQ=25)
                                                cATTACAGTTGTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGt                                                                                  <  2:78879/149‑1 (MQ=255)
                                                      aGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGAc                                                                                          >  1:416351/1‑135 (MQ=255)
                                                      aGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGAc                                                                                          <  2:416351/135‑1 (MQ=255)
                                                             cACTATTGTCAGTTCAGGGTGATGCTCATCAGGAAGATATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAttt                                                                     <  1:282246/149‑1 (MQ=255)
                                                             cACTATTGTCAGTTCAGGGTGATGCTCATCACGCAGGCATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGCCTCCGGCGATATCCGGAACGTCCATGCCGGGGTAGTGATAGACGTTAACGTGCTCCTGGTAttt                                                                     >  2:261994/1‑149 (MQ=21)
                                                                ctcgggcAGTACGGGTTGATGCTGATCAGGGAGGTAGCGGGTCCATGGGGGTACTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGcc                                                                  <  2:244199/143‑1 (MQ=18)
                                                                 aTTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCg                                                                 <  1:477060/149‑1 (MQ=255)
                                                                     tCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTACTGACAGACGCTGCCGTGCCACTGGTATTTGCCGGTGc                                                             >  1:320977/1‑149 (MQ=255)
                                                                     tCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGc                                                             >  1:330734/1‑149 (MQ=255)
                                                                         ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTa                                                         <  2:476685/149‑1 (MQ=255)
                                                                         ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTa                                                         <  2:476654/149‑1 (MQ=255)
                                                                              ggTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACCGGTATTTGCCGGTGCCGTAGCGaa                                                    >  2:7113/1‑149 (MQ=255)
                                                                               gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCCGTAGCGcaa                                                   >  1:420275/1‑149 (MQ=255)
                                                                                 gATGCACATCAGGCTGTTATAGGGTCTCTTGGGGTGCAGGCGGGGTGCGGGGTAAACCGTTAGGACTCCCGAGATATCCGGAACGTCCCTGCCGGTGTGATGTCTGCTGCTAGTGGGCCGCTTGTATTTGCCGGTGCAGTAGCGAAAGa                                                 <  2:347066/149‑1 (MQ=12)
                                                                                     cTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCg                                             <  2:515068/149‑1 (MQ=255)
                                                                                      ttatcaGGTAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATTCTGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCgg                                            <  1:293228/147‑1 (MQ=255)
                                                                                      tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACATACGCTACGCTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCgg                                            >  1:278239/1‑149 (MQ=255)
                                                                                      tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCg                                                                 >  2:580842/1‑128 (MQ=255)
                                                                                      tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCg                                                                 <  1:580842/128‑1 (MQ=255)
                                                                                       catcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGc                                                                   >  1:158753/1‑125 (MQ=255)
                                                                                       catcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGc                                                                   <  2:158753/125‑1 (MQ=255)
                                                                                        atcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGTCTCCGGCGATATCCGGAACGTACCTGCTGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCTGGa                                          <  1:27272/149‑1 (MQ=21)
                                                                                              caggTATAGGGCCCATTGCTGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAgg                                    <  1:492110/149‑1 (MQ=255)
                                                                                                 gTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATg                                 >  1:270114/1‑149 (MQ=255)
                                                                                                    taGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTACTGACAGACGCTACCGTGCCACTGGGATTTGCCGGTGCAGTAGCGCAAGACTCGGGACCGCGGATGCTg                              >  2:210320/1‑149 (MQ=18)
                                                                                                     tgggCCCATTGCGGTCCAGGCGGCGTTCGGCGTATACTGCTAGGACTCCGGCTATATCCGGAACGTCCCTTCCGGTGTAATGACAGACGCTACTGTGCCACTGGTATTTGCCGGTGCAGTAGCGATAGATCCGGGACTCAGCATGCTgg                             <  1:593501/148‑1 (MQ=18)
                                                                                                     aGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGAATCAGGATGCTgg                             >  1:184289/1‑149 (MQ=255)
                                                                                                       ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATAACGCTAGGACCCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTGTTTGCAGGTGCAGTAGCGAAAGATTCAGGAGTCAGGATGCTGCCg                           >  2:292891/1‑149 (MQ=17)
                                                                                                            aTTGCGGTCCTGGCGGCGTTCGGCGTATAGCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGtata                      >  1:636951/1‑149 (MQ=255)
                                                                                                              tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATc                    <  1:220073/149‑1 (MQ=255)
                                                                                                                       ggcggcGTTCGGCGTATACCGCTAGGACTCCGGCGCTATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGCAAGATTCGGGTCTCAGGATGCTGGCGGTATATCGTCATTGcc           <  2:489399/149‑1 (MQ=255)
                                                                                                                           gcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGc       <  2:636951/149‑1 (MQ=255)
                                                                                                                              ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGtt    >  1:231332/1‑149 (MQ=255)
                                                                                                                                gggCGTATACCGCTGGGGCTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTa  <  2:137655/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                       
TCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTA  >  NC_000913_3_pae_tpiA/263957‑264233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑