Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 2,817,170 C→A E112* (GAA→TAA)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,817,1700CA98.9% 291.9 / NA 91E112* (GAA→TAA) yqaBfructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (43/47);  minor base G (1/0);  total (44/47)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCC  >  NC_000913_3_pae_tpiA/2817024‑2817315
                                                                                                                                                  |                                                                                                                                                  
cGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAAc                                                                                                                                                  <  1:529536/149‑1 (MQ=255)
  tCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGCCGACGGCGTAAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCCGCGATGGAGCTTTAACTc                                                                                                                                                >  1:1022552/1‑149 (MQ=255)
  tCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATCATGGCGTAATCCCAGGTGCGGCCGCAATGCCTCAGCGATGGCGCTTTAACTc                                                                                                                                                >  2:520851/1‑149 (MQ=255)
  tCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTc                                                                                                                                                >  2:155916/1‑149 (MQ=255)
  tCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTc                                                                                                                                                >  2:148284/1‑149 (MQ=255)
   cGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTcc                                                                                                                                               <  2:563998/149‑1 (MQ=255)
    ggTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTccc                                                                                                                                              <  1:791284/149‑1 (MQ=255)
      ttGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGGCGTGAGCGGCGGAGGCGACGGCTTCAAAATAATGGAGTAATCCCAGATCCACCAGCAATGCCGCAGCGATGGCGCTTTAACTCCCCg                                                                                                                                            >  1:126403/1‑149 (MQ=255)
         cACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTc                                                                                                                                         <  2:811065/149‑1 (MQ=255)
         cACGACCATACGCTGCGCGCACAAAAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTc                                                                                                                                         <  2:488317/149‑1 (MQ=255)
          aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTcc                                                                                                                                        <  1:155916/149‑1 (MQ=255)
          aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTcc                                                                                                                                        <  1:148284/149‑1 (MQ=255)
          aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTcc                                                                                                                                        >  1:1295831/1‑149 (MQ=255)
          aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATAGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCGGCAATGCCTCACCGATGGCGCTTTAACTCCCCGTTcc                                                                                                                                        >  1:1424863/1‑149 (MQ=255)
              ccATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACa                                                                                                                                    >  2:144810/1‑149 (MQ=255)
              ccATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTCTGAAGTGATCGGCAGCGGCGACGGCGGCAAAATAATGGAGTAATCCCAGATGCGCCAGCAATGCCTCCACGATTGCGCTTTATCTCCCCGTTCCTCCa                                                                                                                                    >  2:1356692/1‑149 (MQ=255)
               gttACGGTGCGCTCACAACAAAAATGTGTCTGGCGCGCGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAg                                                                                                                                   <  2:1204861/147‑1 (MQ=255)
               cagaCGCGGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGACAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAg                                                                                                                                   <  1:111575/146‑1 (MQ=255)
                aTACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGc                                                                                                                                  <  2:1227730/149‑1 (MQ=255)
                  aCGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCa                                                                                                                                >  1:15994/1‑149 (MQ=255)
                    gCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCAtt                                                                                                                              <  1:574354/149‑1 (MQ=255)
                      tGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTgg                                                                                                                            <  1:415703/149‑1 (MQ=255)
                       taccggacaaaaAAAGAGTGTCTGGCGCGGGTTTATGGCGTTTGACGTGATCGGCAGCGACGACGTCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTggg                                                                                                                           <  1:105976/143‑1 (MQ=255)
                        cgcgcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGc                                                                                                                          <  1:144810/149‑1 (MQ=255)
                        cgcgcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTAAAAATAATGGCGTAATTCCAGGTGAGCCAGCAATGCCTCAGCGATAGCGCTTTAACTCCCCGTTCCTACAGACATTGGGc                                                                                                                          >  1:1285463/1‑149 (MQ=255)
                          cgcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAAGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcga                                                                                                                        <  1:1005506/149‑1 (MQ=255)
                           gcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgac                                                                                                                       >  1:473289/1‑149 (MQ=255)
                             acaacaAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgacga                                                                                                                     >  1:799001/1‑149 (MQ=255)
                                   aaaaTGTGTCGGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGc                                                                                                               <  1:255590/149‑1 (MQ=255)
                                    aaaTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGcc                                                                                                              >  2:1301158/1‑149 (MQ=255)
                                       tgtgTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATCGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAAc                                                                                                           <  1:196477/149‑1 (MQ=255)
                                         tgtCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACtt                                                                                                         >  2:1024874/1‑149 (MQ=255)
                                         tgtCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACtt                                                                                                         <  1:1301158/149‑1 (MQ=255)
                                                cgcgGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCGCAAGTGCGCCAGCACTGCCTCACGGTTGGCGCTTTAACTCCCCGTTCCTACAGCCCTTGGACGACGACCATGCGCACTTTTcactc                                                                                                  >  1:1424498/1‑147 (MQ=255)
                                                 gcgGGTGTATGGAGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTcaccac                                                                                                 >  2:860220/1‑149 (MQ=255)
                                                 gcgGATTTATGGTGTTTAACGTCATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCATCGATGGCGCCTTAACCCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTcaccac                                                                                                 <  2:949004/149‑1 (MQ=255)
                                                          tGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGATGACCATGCCAACTTTTCACCACATCAACAag                                                                                        >  1:983151/1‑149 (MQ=255)
                                                           ggTGTTTGCCGTGATCGCCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAaga                                                                                       <  1:24874/149‑1 (MQ=255)
                                                           ggTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAaga                                                                                       <  1:822748/149‑1 (MQ=255)
                                                             tgtTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAgg                                                                                     <  2:1115424/149‑1 (MQ=255)
                                                               tttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACCTCAACAAGAGGaa                                                                                   >  1:883995/1‑149 (MQ=255)
                                                                  gACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCg                                                                                <  1:741450/149‑1 (MQ=255)
                                                                   aCGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCTCCGTTACTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCgg                                                                               <  1:1138182/149‑1 (MQ=255)
                                                                   aCGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACtt                                                                                                         <  2:1338543/123‑1 (MQ=255)
                                                                   aCGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACtt                                                                                                         >  1:1338543/1‑123 (MQ=255)
                                                                     gTGATCGGCAGCGGTGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGtt                                                                             <  1:746410/149‑1 (MQ=255)
                                                                     gTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGACAGCAGtt                                                                             >  2:535054/1‑149 (MQ=255)
                                                                            gCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCt                                                                      >  1:1373722/1‑149 (MQ=255)
                                                                                 gacgacGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCACTGCCTCAGCGATGGCGCTCTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCca                                                                 <  1:1097601/149‑1 (MQ=255)
                                                                                             aaaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActtc                                                    >  1:235386/1‑149 (MQ=255)
                                                                                             aaaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActtc                                                    >  1:1175883/1‑149 (MQ=255)
                                                                                              aaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActtct                                                   >  2:596741/1‑149 (MQ=255)
                                                                                              aaaTAATGGCGTAATCACAGGCGCGCCAGCAATGCCTCAGCGATGGCGCTTCGACTCCCGGTTCCTACAGCCAGTAGGGGACGACCATGCCAACTTTTCACCACCACAACAAGAGGAAGCGGTACGACGCTATCCCGCAGCCTActcct                                                   >  2:896781/1‑149 (MQ=255)
                                                                                                ataatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTa                                                 <  1:520851/149‑1 (MQ=255)
                                                                                                agaatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTa                                                 <  2:447566/147‑1 (MQ=255)
                                                                                                 taatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTag                                                >  1:299707/1‑148 (MQ=255)
                                                                                                   atGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTg                                              <  1:1109247/149‑1 (MQ=255)
                                                                                                      gCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCCCATCATCAAGAGGGAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCtt                                           >  2:419186/1‑149 (MQ=255)
                                                                                                             cccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACTACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGtttt                                    <  1:515621/149‑1 (MQ=255)
                                                                                                              ccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAaga                                                                                       >  2:502235/1‑98 (MQ=255)
                                                                                                              ccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAaga                                                                                       <  1:502235/98‑1 (MQ=255)
                                                                                                              ccAGGTGCGCCAGCAATGCATCAGCGATGGCCCTTTAACTCCCCGTTCCTCCAGCCATAGCGCGACGAAAATACCAACTTTTCACCACATCAACAAAAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTGCGGttttt                                   >  2:417950/1‑149 (MQ=255)
                                                                                                               cAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGtttttt                                  >  1:620570/1‑149 (MQ=255)
                                                                                                                  gTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTCTCCCGAAGCAAACTCCTGAATGCTTCTGttttttacc                               >  1:30414/1‑146 (MQ=255)
                                                                                                                    ggggcAGCAATGCCTCGGCGATGTCGCTTTACCTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAcgc                             <  1:208713/145‑1 (MQ=255)
                                                                                                                    gcgcCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAcgc                             <  1:100199/149‑1 (MQ=255)
                                                                                                                      gcCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTCGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACgcgc                           <  2:1342348/149‑1 (MQ=255)
                                                                                                                       ccAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCt                          <  1:1176831/149‑1 (MQ=255)
                                                                                                                       ccAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCt                          >  1:1149676/1‑149 (MQ=255)
                                                                                                                        cAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTg                                              >  1:1095794/1‑128 (MQ=255)
                                                                                                                        cAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTg                                              <  2:1095794/128‑1 (MQ=255)
                                                                                                                         aGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTaa                        >  2:1389879/1‑149 (MQ=255)
                                                                                                                          ggaaTGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAAc                       <  1:593715/147‑1 (MQ=255)
                                                                                                                            aaTGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCAtt                                                                                                                              <  2:894216/45‑1 (MQ=255)
                                                                                                                            aaTGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCAtt                                                                                                                              <  2:894208/45‑1 (MQ=255)
                                                                                                                            aaTGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCAtt                                                                                                                              >  1:894208/1‑45 (MQ=255)
                                                                                                                            aaTGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCAtt                                                                                                                              >  1:894216/1‑45 (MQ=255)
                                                                                                                            aaTGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGc                     >  1:1280655/1‑149 (MQ=255)
                                                                                                                             aTGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCa                    <  2:1175883/149‑1 (MQ=255)
                                                                                                                                 cTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCg                >  2:1070409/1‑149 (MQ=255)
                                                                                                                                  tCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCAt                                                                                                                 >  2:390376/1‑52 (MQ=255)
                                                                                                                                  tCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCAt                                                                                                                 <  1:390376/52‑1 (MQ=255)
                                                                                                                                  tCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCgg               >  1:266675/1‑149 (MQ=255)
                                                                                                                                  tCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTCGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCCGCATACTTCTTACTGCTTCTGTTTTTCTCCGCGCTAACCCATGCgg               >  2:435840/1‑149 (MQ=255)
                                                                                                                                  cacgCGATGGCGCTTTAGCTCTCCTTACCTACAGCCAGTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCgg               <  2:1022552/146‑1 (MQ=255)
                                                                                                                                     gCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTc            <  2:1149676/149‑1 (MQ=255)
                                                                                                                                     gCGATGCCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTc            <  2:15994/149‑1 (MQ=255)
                                                                                                                                         tGGCGCTGTAAGGCCGCGTTCCCACAGCCATTGGGTGACGACCATGCCAACTTTTCGCTACATCATCAAGATTGAGCGGTTCTACGCTATCCAGCAGTATAGTGCTTACGGCTTTTGTTTTTTCACGCGCTAACGCATGCGGGTCgaga        <  2:329753/149‑1 (MQ=255)
                                                                                                                                              cTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGc   <  2:266675/149‑1 (MQ=255)
                                                                                                                                               tttAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCgg               >  1:143218/1‑136 (MQ=255)
                                                                                                                                               tttAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATACACAGCTATATTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGCCGAGATCGGcc  >  1:1420584/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCC  >  NC_000913_3_pae_tpiA/2817024‑2817315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: