Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	2,106,741	A→T	S162S (TCT→TCA)	wbbH ←	O‑antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	2,106,741	0	A	T	100.0%	266.0 / NA	79	S162S (TCT→TCA)	wbbH	O‑antigen polymerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (37/42);  total (37/42)

TATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTCA  >  NC_000913_3_pae_tpiA/2106599‑2106886                                                                                                                                                |                                                                                                                                                   tATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTACCGATAAAT‑TAATACTTTCCTTTGTATTAGTAAATTTTTTTGCCCaa                                                                                                                                              >  1:993697/1‑149 (MQ=255)   ttAACACCTACGATGAATGCATAAGAGATGATAACAATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCaaat                                                                                                                                            >  2:1072379/1‑149 (MQ=255)        acCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAaataaata                                                                                                                                       >  2:278557/1‑149 (MQ=255)          cTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAAtaaataaa                                                                                                                                     <  2:935155/149‑1 (MQ=255)          cTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAAAAAAC‑CAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAAtaaataaa                                                                                                                                     <  1:282690/149‑1 (MQ=255)           tACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAataaataa                                                                                                                                      <  2:1446542/147‑1 (MQ=255)           tACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAataaataa                                                                                                                                      >  1:1446542/1‑147 (MQ=255)           tACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAg                                                                                                                                    >  2:684519/1‑149 (MQ=255)            aCGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGc                                                                                                                                   <  1:407088/149‑1 (MQ=255)              gATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCTAATAAATAAAGGaa                                                                                                                                 >  2:46834/1‑149 (MQ=255)              gATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCaa                                                                                                                                 <  2:144361/149‑1 (MQ=255)                 gAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAag                                                                                                                              >  1:950829/1‑149 (MQ=255)                    tGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGCTGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAggtag                                                                                                                           <  2:1101695/149‑1 (MQ=255)                      cATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTTACCAAATAAATAAAGCAAAAGTAGtt                                                                                                                         >  2:1188033/1‑149 (MQ=255)                      cATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGtt                                                                                                                         >  1:342817/1‑149 (MQ=255)                       aTAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGGTGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTCTTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTCGttg                                                                                                                        >  1:317970/1‑148 (MQ=255)                        tAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAg                                                                                                                       >  2:204384/1‑149 (MQ=255)                          agagATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑aa                                                                                                                    >  2:782883/1‑149 (MQ=255)                          agagATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑aa                                                                                                                    >  2:60626/1‑149 (MQ=255)                             gatgatAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGa                                                                                                                 <  2:304699/149‑1 (MQ=255)                               tgatAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGAtt                                                                                                               <  1:364442/149‑1 (MQ=255)                                 atAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTTCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTgg                                                                                                             <  2:716308/149‑1 (MQ=255)                                 atAACCATAAAAAAAATTTGCTTACAAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTTGATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTgg                                                                                                             <  1:198935/149‑1 (MQ=255)                                  tAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGc                                                                                                            <  1:253514/149‑1 (MQ=255)                                   aaCCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCt                                                                                                           >  1:1323703/1‑149 (MQ=255)                                       aTAAAGACAATTTGCTCACTAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAAAAAGTAAAG‑TAAATGTTTTACTTACCGTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCAt                                                                                                       <  1:339827/149‑1 (MQ=255)                                          aaGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGAACAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTa                                                                                                    <  2:1033545/149‑1 (MQ=255)                                           ataCAATCTGCTAACCAGTATTCAAAATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTTTATTATTAAATTTTTTTGACCAAATACATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTAt                                                                                                   <  1:1001648/147‑1 (MQ=255)                                           aGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACTTTATAAATACAACAAAACGAGTTAT‑AATGAATGGATTCAttttt                                                                                                   >  2:579788/1‑145 (MQ=255)                                           aGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTAt                                                                                                   <  1:997466/149‑1 (MQ=255)                                                    gCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGaaa                                                                                          <  1:473681/149‑1 (MQ=255)                                                     cTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAAt                                                                                         >  2:699451/1‑149 (MQ=255)                                                     cTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAAt                                                                                         <  1:751210/149‑1 (MQ=255)                                                      ttACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAAtt                                                                                        >  1:289614/1‑149 (MQ=255)                                                      ttACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGAAGTTAG‑AATGATTGCCTGCATGTATGCTggaaaact                                                                                        >  2:588717/1‑142 (MQ=255)                                                          aaGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCtt                                                                                    <  2:352221/148‑1 (MQ=255)                                                            gTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGa                                                                                  <  1:537871/149‑1 (MQ=255)                                                             tATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTAt                                                                                                   >  1:953571/1‑131 (MQ=255)                                                             tATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTAt                                                                                                   <  2:953571/131‑1 (MQ=255)                                                             tATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAt                                                                                 >  1:55038/1‑149 (MQ=255)                                                               atCAGTATCATTGCAAAGATGAATACAAGTCAAACAAGCAAAG‑TAAATGTTTTACTTACCTTTGTATCCGTCAATTTTTTTGGCCATCTAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAtgt                                                                               <  1:341159/148‑1 (MQ=255)                                                                   gTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGATTTTCATACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCt                                                                           >  1:1368896/1‑149 (MQ=255)                                                                    tataATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCtt                                                                          >  1:571473/1‑149 (MQ=255)                                                                     ataATTGAAAAGATGAATAAAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTc                                                                         <  2:161561/149‑1 (MQ=255)                                                                            gaaaGAAAAAAACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCa                                                                  <  2:571473/148‑1 (MQ=255)                                                                                 aTGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCAtc                                                             >  2:1300988/1‑149 (MQ=255)                                                                                  tGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCAtct                                                            >  1:1143021/1‑149 (MQ=255)                                                                                   gAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCAtctc                                                           <  1:782883/149‑1 (MQ=255)                                                                                   gAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCAtctc                                                           <  1:60626/149‑1 (MQ=255)                                                                                   gAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTCAGAAATTTCTTGATGTGTCTTCAACATCAGCAtctc                                                           >  1:478715/1‑149 (MQ=255)                                                                                   gAAAACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCAtctc                                                           <  1:469176/149‑1 (MQ=255)                                                                                    aaTACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATCAATCAAGCTAAAGTA‑TAAGTATTGGTTTGCTGCATGAATGCTGAGAAATTTCTTGATGTTCCTTCAACATCAGCATctct                                                          <  2:1033008/149‑1 (MQ=255)                                                                                     aTACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCAACTCtt                                                         <  2:1257225/149‑1 (MQ=255)                                                                                      tACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTa                                                        <  2:55038/149‑1 (MQ=255)                                                                                         caTAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACTAAATAAATAAAGCAAAAGTAATTAG‑AATCATTGGCACCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACACCAGCATCCCTTATCa                                                     >  2:1098767/2‑149 (MQ=255)                                                                                         aaTAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTa                                                        <  2:643670/146‑1 (MQ=255)                                                                                         aaTAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTa                                                        >  1:643670/1‑146 (MQ=255)                                                                                           tAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAGATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCaaa                                                   >  1:74718/1‑149 (MQ=255)                                                                                           tAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACCTTAGCATCTCTTATTaaa                                                   >  2:796964/1‑149 (MQ=255)                                                                                             aaaaCAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATTGATGCTGAGAAATTTCTTCATCACACTTCAACATCAGCATCTCTTATCAACtt                                                 >  1:1279053/1‑149 (MQ=255)                                                                                             aaaaCAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAAtt                                                 <  2:534769/149‑1 (MQ=255)                                                                                               aaCAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCa                                               <  1:684519/149‑1 (MQ=255)                                                                                                   agtaaag‑taaATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCAtata                                           <  1:204384/149‑1 (MQ=255)                                                                                                     taaag‑taaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGc                                         <  1:1368803/149‑1 (MQ=255)                                                                                                         aggaaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaa                                      <  2:779653/146‑1 (MQ=255)                                                                                                           taaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACAAAATAAATAAAGCAAAAGAAGTTAG‑AATGATTGGCTGAATGGATGCTGAGAAATTTCTTGATTTGTCTTCAACAGCAGCATCTCTTATCACATTCATATCGCtaagg                                    >  2:577758/1‑148 (MQ=255)                                                                                                                 ttttACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTa                              >  1:225885/1‑149 (MQ=255)                                                                                                                     ccttacCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGCCGCCGTTCGAATGGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTcc                          >  1:1420966/2‑149 (MQ=255)                                                                                                                         acCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCGGCATCTCTTATCAAATTCATATAGCta                                       <  2:110187/132‑1 (MQ=255)                                                                                                                         acCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCGGCATCTCTTATCAAATTCATATAGCta                                       >  1:110187/1‑132 (MQ=255)                                                                                                                           cTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACt                    >  1:992260/1‑149 (MQ=255)                                                                                                                             ttGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTgg                  >  1:388044/1‑149 (MQ=255)                                                                                                                             ttGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTgg                  <  1:1235865/149‑1 (MQ=255)                                                                                                                               gTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTa                <  1:1016885/149‑1 (MQ=255)                                                                                                                               gTAATAGTAAAGTTTTTTGACCAAATAAAAAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTa                <  2:480281/149‑1 (MQ=255)                                                                                                                                   tAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTTCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTa            <  2:1095070/149‑1 (MQ=255)                                                                                                                                   gaGTAAATGTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTa            <  2:1055823/148‑1 (MQ=255)                                                                                                                                      tAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTGCtt         >  1:738424/1‑149 (MQ=255)                                                                                                                                        aaTTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCGAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTaa       <  2:55486/149‑1 (MQ=255)                                                                                                                                             ttttGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGTAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTCa  >  1:646629/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                   TATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAG‑AATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTCA  >  NC_000913_3_pae_tpiA/2106599‑2106886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑