New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 34978354 (1.020)6 (0.190)
+CTACAGTTCAGTTCACCCCGTAGGCCTGATAAGTCGCGTCAGCGGCGCATCAGGCATCG
4/166 4.8 16.0% noncoding (179/365 nt) REP25 (repetitive extragenic palindromic) element; contains 8 REP sequences REP25 (repetitive extragenic palindromic) element; contains 8 REP sequences
?NC_000913_3_pae_tpiA = 349972 NA (NA)intergenic (+400/‑40) prpB/prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAA  >  NC_000913_3_pae_tpiA/349638‑349927
                                                                                                                                                 |                                                                                                                                                
tACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAc                                                                                                                                               <  2:90930/149‑1 (MQ=39)
 aCGAGGAGCACTGAACTGGAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGGGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAcc                                                                                                                                              <  1:144684/149‑1 (MQ=255)
      gTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGAc                                                                                                                                                  >  2:480452/1‑140 (MQ=39)
      gTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGAc                                                                                                                                                  <  1:480452/140‑1 (MQ=39)
           gtggaCTGGAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGAt                                                                                                                                    <  2:679456/145‑1 (MQ=255)
           cTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCCATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGAACAGTACAGGAGGTCAGATAAGACGCACAGCTCGGCAACCGACAACCGATCCCGGAt                                                                                                                                    >  1:126027/1‑149 (MQ=11)
            tGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATg                                                                                                                                   >  1:746296/1‑149 (MQ=39)
            tGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATACCTGAAGCGCCGCTGCCGAGACCTACCAGACCACCCGGGAGCACGGAACTGTAGGGCAGATACGCGGCATAGCGTCGCATACGAAACCCAATGcataact                                                                                                                                   >  2:46214/1‑143 (MQ=2)
               aCTGTAGGTCGAATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATgcgc                                                                                                                                <  2:828282/149‑1 (MQ=37)
                cTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgcc                                                                                                                               >  1:429210/1‑149 (MQ=39)
                       tCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAcg                                                                                                                        >  2:165164/1‑149 (MQ=39)
                           aaaaGACGGGTGGGGTCGCAGCCGACAACCGATGCCTGATGCGTCGCTGACGGGACTTATCAGGCCTACGGGGTGCACGGAACTGTAGGTCGGATAAGACGCACAGCGTCGCATCCGACAACCGATGCCTGATGCGCTGCTGACGCGAc                                                                                                                    <  2:113209/147‑1 (MQ=11)
                            tAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGCCTTATCCTGCCGTCGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACt                                                                                                                   >  1:134390/1‑149 (MQ=11)
                               gACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGACTCCCCACTGACACCACTTAt                                                                                                                >  2:833519/1‑149 (MQ=11)
                                aCGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTtctc                                                                                                               >  2:94912/1‑149 (MQ=38)
                                aCGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATc                                                                                                               >  2:427916/1‑149 (MQ=38)
                                  ggATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAg                                                                                                             <  2:569019/149‑1 (MQ=35)
                                  ggATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAg                                                                                                             <  1:550717/149‑1 (MQ=35)
                                         gTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACg                                                                                                      <  1:686931/149‑1 (MQ=32)
                                               tCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGCTGCCTGATGCCCCGCTGACGCGGCGTGTCACGCCTACGGGGGGc                                                                                                >  2:158330/1‑149 (MQ=11)
                                                     aaCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTg                                                                                                                                      >  2:387131/1‑105 (MQ=33)
                                                     aaCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGaa                                                                                          <  1:165164/149‑1 (MQ=35)
                                                     aaCCGATGCCGGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGaa                                                                                          <  1:44453/149‑1 (MQ=25)
                                                      aCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCACCCGACAACCGATGCCTGCTGCGCCGCTGACGCGCCTTCTCAGGCCTACGGGGGGCCATGAAc                                                                                         >  2:287392/1‑149 (MQ=11)
                                                        cgtagCCGGAGGCGGCGCTGACGGGATTTTTCGGGACTACGGGGGGCAGTGAGCTGGAGGGCTGATAAGGGGCCTGGGGTTGCATCCGACAGCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCTTACGGGGTGCACTGAACTg                                                                                       <  2:906124/145‑1 (MQ=2)
                                                           tGCCGGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAg                                                                                    <  2:855376/149‑1 (MQ=25)
                                                              cTGATGCGCCTCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTc                                                                                 <  1:404255/149‑1 (MQ=25)
                                                              cTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTc                                                                                 <  1:89542/149‑1 (MQ=35)
                                                               tGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAgg                                                                                                            >  1:542208/1‑121 (MQ=33)
                                                               tGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAgg                                                                                                            <  2:542208/121‑1 (MQ=33)
                                                                      ccgcTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa                                                                         <  2:429210/149‑1 (MQ=35)
                                                                       cgcTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGAc                                                                        >  2:567232/1‑149 (MQ=35)
                                                                         cTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGc                                                                      <  1:567232/149‑1 (MQ=35)
                                                                                 aCTTATCAGGCCTACGGGGTGCACTGAACCGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGTGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc                                                              <  2:717535/149‑1 (MQ=17)
                                                                                 aCTTATAAGCCCTACGGAGTGCACTTAGCGTTATGTAGGATATGACGCATGGCGTCGCAGCCGACAACCGATGCCTGATGCGCCGCTGACGCTACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc                                                              <  2:210001/149‑1 (MQ=11)
                                                                                  gttATCAGGCGTACGGGGTGCACTGAACTGGAGGTCGGATAAGACGCATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCg                                                             <  2:201105/148‑1 (MQ=12)
                                                                                      tCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATc                                                         <  2:746296/149‑1 (MQ=32)
                                                                                         ggCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa                                                      <  1:131314/149‑1 (MQ=32)
                                                                                         ggCCTACGAGGTGCACTGAACTGTAGGTCGGAAAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCCGACGCTACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa                                                      <  1:131416/149‑1 (MQ=255)
                                                                                            cTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACaa                                                   >  1:278916/1‑149 (MQ=32)
                                                                                                       aCTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa                                                                         >  2:205063/1‑116 (MQ=32)
                                                                                                         tGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGCATAAGACGCATAGCGTCGGATCCGACAACCGATGCCTGATg                                      >  1:22688/1‑149 (MQ=255)
                                                                                                               ggaGGTCGCACAAGACGCATAGCCTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCGTACGGGGTGCACTGAACTGTAGGTCGGATAACACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccgc                                <  2:277896/147‑1 (MQ=11)
                                                                                                               ggaGGGCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccgc                                <  1:899093/147‑1 (MQ=37)
                                                                                                                   gTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGGGTCGCATCCGACAACCGATACCTGATGCGACGCTGAc                            >  1:808914/1‑149 (MQ=255)
                                                                                                                    tCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGt                                                               >  1:310614/1‑113 (MQ=33)
                                                                                                                       gATAACACGCATTGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCTATGCCTGATTCGCCGCTGACGCGa                        <  1:810922/149‑1 (MQ=255)
                                                                                                                           aGACGCAGGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTa                    <  2:924252/149‑1 (MQ=17)
                                                                                                                               gCAGAGCGGCGCATCCGCCAACCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACGGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAg                <  1:880251/149‑1 (MQ=11)
                                                                                                                                 attgCGTCGCACCCGACAACCGATGCCTGATGCGCGGCTGACGCGACGTCTCAGGCCTACGGGGTGCACTGAACTGTAGGTTGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGc              <  2:725055/146‑1 (MQ=11)
                                                                                                                                    ccGTCGCATGCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTa           <  1:506481/148‑1 (MQ=17)
                                                                                                                                      gTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACg         <  1:350776/149‑1 (MQ=32)
                                                                                                                                          cATCGGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGt     <  2:720150/149‑1 (MQ=17)
                                                                                                                                             ccGACAACCGATGCCTGATGCGCCGTCGACGGGACTTATCAGGCCCAAGAAGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaa  <  1:215070/149‑1 (MQ=11)
                                                                                                                                                 cAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAgg               <  2:647385/132‑1 (MQ=32)
                                                                                                                                                 cAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAgg               >  1:647385/1‑132 (MQ=33)
                                                                                                                                                 |                                                                                                                                                
TACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAA  >  NC_000913_3_pae_tpiA/349638‑349927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.